Application of specific IgG detection in intraocular fluid for the laboratory diagnosis of ocular toxocariasis_Chinese Journal of Ocular Fundus Diseases

Authors：

WangZhujian , ZhouMin , JiJian , 沈健 , 汤荔 ,  曹文俊 , 徐格致

Clinical Laboratory, The EENT Hospital of Fudan University, Shanghai 200031, China;

Corresponding author：

曹文俊, Email: wenjun.cao@fdeent.org

Keywords：

Toxocariasis/diagnosis; Larva migrans; Toxascariasis; Immunoglobulin G/diagnostic use

DOI：

10.3760/cma.j.issn.1005-1015.2015.01.011

Video：

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Objective To evaluate the clinical value of detecting specific IgG against Toxocara canis in intraocular fluid for the diagnosis of ocular toxocariasis(OT). Methods Fifty patients diagnosed as having OT(OT group), 152 otolaryngology patients (serum control group) and 70 other oculopathy patients (intraocular fluid control group) were enrolled in the study. Intraocular fluids(28 aqueous humor and 22 vitreous humor) and serum samples of OT group were analyzed for specific IgG against Toxocara canis by enzymelinked immunosorbent assay, so were the intraocular fluids(46 aqueous humor and 24 vitreous humor) and 152 serum samples of two control group. Specific IgG level was compared between paired serum and intraocular fluids of OT group. Results 68.00% serum samples of OT group were positive for specific IgG against Toxocara canis and the U value was 20.42±17.01. The positive rate was 88.00% and U value 25.72±23.04 in intraocular fluids. In serum control group, it was 2.63% and 2.37±2.71 respectively. The intraocular fluids were negative and U value 0.69±0.34 in intraocular fluid control group. The difference of specific IgG level was proved significant between OT group and control group in both serum and intraocular fluid (Z=8.962, 8.120; P=0.000, 0.000). Twenty-eight patients (56.00%) were positive for specific IgG in both serum and intraocular fluid. Six patients (12.00%) were positive only in serum, and 16 patients (32.00%) only in intraocular fluid. The positive rate was significantly higher in intraocular fluid than in serum (χ²=4.720, P=0.028). Moreover, 64.00% intraocular fluid showed higher specific IgG level than pared serum. Conclusion The complementary detection of intraocular specific IgG have referential value in diagnosing ocular toxocariasis.

Citation： WangZhujian, ZhouMin, JiJian. Application of specific IgG detection in intraocular fluid for the laboratory diagnosis of ocular toxocariasis. Chinese Journal of Ocular Fundus Diseases, 2015, 31(1): 41-44. doi: 10.3760/cma.j.issn.1005-1015.2015.01.011 Copy

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1. Stewart JM. Prevalence clinical features, and causes of vision loss among patients with ocular toxocariasis[J]. Retina, 2005, 25(8):1005-1013.
2. Yokoi K, Goto H, Sakai J, et al. Clinical features of ocular toxocariasis in Japan[J].Ocul lmmunol lnflamm, 2003, 11(4):269-275.
3. Woodhall D, Starr MC, Montgomery SP, et al.Ocular toxocariasis:epidemiologic, anatomic, and therapeutic variations based on a survey of ophthalmic subspecialists[J].Ophthalmology, 2012, 119(6):1211-1217.
4. Kwon SI, Lee JP, Park SP, et al.Ocular toxocariasis in Korea[J]. Jpn J Ophthalmol, 2011, 55:143-147.
5. Chieffi PP, dos Santos SV, de Queiroz ML, et al.Human toxocariasis:contribution by Brazilian reasearchers[J].Rev Inst Med Trop Sao Paulo, 2009, 51(6):301-308.
6. Rubinsky-Elefant G, Hirata CE, Yamamoto JH, et al.Human toxocariasis:diagnosis, wordwide seroprevalences and clinical expression of the systemic and ocular forms[J].Ann Trop Med Parasitol, 2010, 104(1):3-23.
7. Centers for Disease Control and Prevention(CDC).Ocular toxocariasis-United States, 2009-2010[J]. MMWR Morb Mortal Wkly Rep, 2011, 60(22):729-733.
8. Zhou M, Chang Q, Gonzales JA., et al. Clinical characteristics of ocular toxocariasis in Eastern China[J].Graefe's Arch Clin Exp Ophthalmol, 2012, 250(9):1373-1378.
9. Macpherson CN.The epidemiology and public health importance of toxocariasis:a zoonosis of global importance[J]. Int J Parasitol, 2013, 43(12-13):999-1008.
10. Fillaux J, Magnaval JF. Laboratory diagnosis of human toxocariasis[J]. Vet Parasitol, 2013, 193(4):327-336.
11. Ahn SJ, Woo SJ, Jin Y, et al. Clinical features and course of ocular Toxocariasis in adults. PLoS Negl Trop Dis, 2014, 8(6):2938. http://dx.plos.org/10.1371/journal.pntd.0002938.
12. Arevalo JF, Espinoza JV, Arevalo FA.Ocular toxocariasis[J].J Pediatr Ophthalmol Strabismus, 2013, 50(2):76-86.
13. de Visser L, Rothova A, de Boer JH, et al.Diagnosis of ocular toxocariasis by establishing intraocular antibodyproduction[J]. Am J Ophthalmol, 2008, 145(2):369-374.
14. Akiyama T, Ohta N. Parasite-specific antibody profile in the aqueous humor of rabbits with ocular toxocariasis[J].Parasitol Int, 2010, 59(2):112-120.

Chinese Journal of Ocular Fundus Diseases

Application of specific IgG detection in intraocular fluid for the laboratory diagnosis of ocular toxocariasis

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