The effects of Crumbs proteins on zebrafish photoreceptors_Chinese Journal of Ocular Fundus Diseases

Authors：

 Fu Jinling

Department of Ophthalmology, The Second Hospital of Jilin University, Changchun 130041, China;

Corresponding author：

Fu Jinling, Email: fujinling1981@163.com

Keywords：

Photoreceptor cells, vertebrate/physiology; Zebrafish; Animal experimentation

DOI：

10.3760/cma.j.issn.1005-1015.2017.02.014

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To observe the effects of Crumbs (Crb) proteins on different types of zebrafish photoreceptors. Methods The retinal cell population dynamics of adult wild-type zebrafish and Tg (RH2-2:Crb2b-sf^-EX/RH2-2:GFP)^pt108b transgenic zebrafish (called pt108b zebrafish for short) were evaluated by monitoring the densities of three categories of retinal photoreceptors (rod cells, UV cone cells and RGB cone cells) in different retinal regions, which were visualized by Feulgen nuclear staining histology technique. Results The wild-type zebrafish retinal photoreceptor cell densities are generally higher in the central region than the peripheral regions. Compared with wild-type zebrafish, pt108b zebrafish had much less RGB cone cells at the top of outer nuclear layer, and no RGB cone cells at the central and intermediate regions of retina. While pt108b zebrafish had normal density of UV cone cells at the top of rods and the bottom of outer limiting membrane, they had much higher density of rods. Conclusions Crb proteins may affect the zebrafish retinal cell densities of different photoreceptor types.

Citation： Fu Jinling. The effects of Crumbs proteins on zebrafish photoreceptors. Chinese Journal of Ocular Fundus Diseases, 2017, 33(2): 172-175. doi: 10.3760/cma.j.issn.1005-1015.2017.02.014 Copy

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1. Cepko C. Neuroscience: seeing the light of day[J]. Science, 2010, 329(5990): 403- 404. DOI: 10.1126/science.1194086.
2. Alves CH, Pellissier LP, Wijnholds J. The CRB1 and adherens junction complex proteins in retinal development and maintenance[J]. Prog Retin Eye Res, 2014, 40: 35-52. DOI: 10.1016/ j.preteyeres.2014.01.001.
3. Beryozkin A, Zelinger L, Bandah-Rozenfeld D.Mutations in CRB1 are a relatively common cause of autosomal recessive early-onset retinal degeneration in the Israeli and Palestinian populations[J]. Invest Ophthalmol Vis Sci, 2013, 54(3): 2068-2075. DOI: 10.1167/v iovs.12-11419.
4. Bujakowska K, Audo I, Mohand-Saïd S, et al. CRB1 mutations in inherited retinal dystrophies[J]. Hum Mutat, 2012, 33(2): 306-315. DOI: 10.1002/humu.21653.
5. Azam M, Collin RW, Malik A, et al. Identification of novel mutations in pakistani families with autosomal recessive retinitis pigmentosa[J]. Arch Ophthalmol, 2011, 129(10): 1377-1378. DOI: 10.1001/archophthalmol.2011.290.
6. Clark GR, Crowe P, Muszynska D, et al. Development of a diagnostic genetic test for simplex and autosomal recessive retinitis pigmentosa[J]. Ophthalmology, 2010, 117(11): 2169-2177. DOI: 10.1016/j.ophtha.2010.02.029.
7. Siemiatkowska AM, Arimadyo K, Moruz LM, et al. Molecular genetic analysis of retinitis pigmentosa in Indonesia using genome-wide homozygosity mapping[J]. Mol Vis, 2011, 17: 3013-3024.
8. Tappeiner C, Gerber S, Enzmann V, et al. Visual acuity and contrast sensitivity of adult zebrafish[J]. Front Zool, 2012, 9(1): 10. DOI: 10.1186/1742-9994-9-10.
9. Alvarez Y, Chen K, Reynolds AL, et al. Predominant cone photoreceptor dysfunction in a hyperglycaemic model of non-proliferative diabetic retinopathy[J]. Dis Model Mech, 2010, 3(3-4): 236-245. DOI: 10.1242/dmm.003772.
10. Huang YY, Haug MF, Gesemann M, et al. Novel expression patterns of metabotropic glutamate receptor 6 in the zebrafish nervous system[J/OL].PLoS One, 2012, 7(4): e35256[2012-04-16]. . DOI: 10.1371/ journal.pone.0035256.
11. Robinson J, Schmitt EA, Harosi FI, et al. Zebrafish ultraviolet visual pigment: absorption spectrum, sequence, and localization[J]. Proc Natl Acad Sci USA, 1993, 90(13): 6009-6012.
12. Zou J, Wang X, Wei X. Crb apical polarity proteins maintain zebrafish retinal cone mosaics via intercellular binding of their extracellular domains[J]. Dev Cell, 2012, 22(6): 1261-1274. DOI: 10.1016/j.devcel.2012.03.007.
13. Fu J, Fang W, Zou J, et al. A robust procedure for distinctively visualizing zebrafish retinal cell nuclei under bright field light microscopy[J]. J Histochem Cytochem, 2013, 61(3): 248-256. DOI: 10.1369/0022155412471535.
14. Zou J, Lathrop K L, Sun M, et al. Intact retinal pigment epithelium maintained by Nok is essential for retinal epithelial polarity and cellular patterning in zebrafish[J]. J Neurosci, 2008, 28(50): 13684-13695. DOI: 10.1523/JNEUROSCI.4333-08.2008.
15. Ehrenberg M, Pierce EA, Cox GF, et al. CRB1: one gene, many phenotypes[J]. Semin Ophthalmol, 2013, 28(5-6): 397-405. DOI: 10.3109/08820538.2013.825277.
16. Gosens I, den Hollander AI, Cremers FP, et al. Composition and function of the Crumbs protein complex in the mammalian retina[J]. Exp Eye Res, 2008, 86(5): 713-726. DOI: 10.1016/j.exer. 2008.02.005.
17. Brockerhoff SE, Fadool JM. Genetics of photoreceptor degeneration and regeneration in zebrafish[J]. Cell Mol Life Sci, 2011, 68(4): 651-659. DOI: 10.1007/s00018-010-0563-8.

Chinese Journal of Ocular Fundus Diseases

The effects of Crumbs proteins on zebrafish photoreceptors

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