Effect of atorvastatin on the expression of chemokine receptor-4 and migration ability of rat bone marrow mesenchymal stem cells cultivated in vitro_Chinese Journal of Ocular Fundus Diseases

Authors：

Wu Bin ,  Chen Song , Zhang Wei , He Guanghui , Wang Jian

Tianjin Eye Hospital，Clinical College of Ophthalmology of Tianjin Medical University, Tianjin Key Laboratory of Ophthalmology and Vision Science, Tianjin Institute of Ophthalmology, Tianjin 300020, China;

Corresponding author：

Chen Song, Email: chensong9999@126.com

Keywords：

Mesenchymal stem cell; Atorvastatin; Receptors, CXCR4; Cell, cultured

DOI：

10.3760/cma.j.issn.1005-1015.2018.06.010

Video：

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ObjectiveTo investigate the effects of migration and expression from chemokine receptor 4 (chemokine receptor-4, CXCR4) of rat bone marrow mesenchymal stem cells (BMSCs) which were pretreated by atorvastatin (ATV) in vitro.MethodsIsolated, cultivated, identified the BMSCs, pretreated P4-P6 of BMSCs with different concentrations of ATV for 12 hours. The experimental group was divided into control group, 0.1 nM/L (group 0.1 nM), 1 nM/L (1 nM group), 10 nM/L (10 nM group), 100 nM/L (100 nM group), 1 000 nM/L (1 000 nM group). The mRNA and protein of CXCR4 were determined by real time-polymerase chain reaction and Western blot. Immunofluoreseence assay were used to detect the expression levels of CXCR4. The migration ability of BMSCs were measured by transwell chamber.ResultsImmunofluoreseence assay showed the protein level of CXCR4 of group 1 nM and 10 nM were significantly higher than the other group. RT-PCR and Western blot showed the protein and mRNA level of CXCR4 in 10 nM was higher than that in group 1 nM. The migration ability of group 10 nM was higher than 1 nM and control group.ConclusionsATV can be dose-dependent promote expression levels of CXCR4 of BMSCs cultivated in vitro.

Citation： Wu Bin, Chen Song, Zhang Wei, He Guanghui, Wang Jian. Effect of atorvastatin on the expression of chemokine receptor-4 and migration ability of rat bone marrow mesenchymal stem cells cultivated in vitro. Chinese Journal of Ocular Fundus Diseases, 2018, 34(6): 575-579. doi: 10.3760/cma.j.issn.1005-1015.2018.06.010 Copy

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8.	王健, 陈松.基质细胞衍生因子-1/CXC趋化因子受体4通路在间充质干细胞治疗糖尿病视网膜病变中的作用研究[J]. 中华眼底病杂志, 2016, 32(6): 663-667. DOI: 10.3760/cma.j.issn.1005-1015.2016.06.029.Wang J, Chen S. Role of stromal cell derived factor-1/CXC chemokine receptor 4 pathway in mesenchymal stem cells therapies in the management of diabetic retinopathy[J]. Chin J Ocul Fundus Dis, 2016, 32(6): 663-667.DOI: 10.3760/cma.j.issn.1005-1015.2016.06.029.
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20.	张惟, 韩琪, 陈松, 等.辛伐他汀对糖尿病大鼠骨髓内皮祖细胞数量和功能的影响[J]. 中华眼科杂志, 2012, 48(11): 1015-1020.DOI: 10.3760/cma.j.issn.0412-4081.2012.11.013.Zhang.Zhang W, Han Q, Chen S, et al. Effect of simvastatin on the amount and function of endothelial progenitor cells from bone marrow in diabetic rats[J]. Chin J Ophthalmol, 2012, 48(11): 1015-1020. DOI: 10.3760/cma.j.issn.0412-4081.2012.11.013.

1. Megaw R, Dhillon B.Stem cell therapies in the management of diabetic retinopathy[J]. Curr Diab Rep, 2014, 14(7): 498. DOI: 10.1007/s11892-014-0498-9.
2. Kavanagh H, Mahon BP. Allogeneic mesenchymal stem cells prevent allergic airway inflammation by inducing murine regulatory T cells[J]. Allergy, 2011, 66(4): 523-531. DOI: 10.1111/j.1398-9995.2010.02509.x.
3. Yan H, Wu M, Yuan Y, et al. Priming of Toll-like receptor 4 pathway in mesenchymal stem cells increases expression of B cell activating factor[J]. Biochem Biophys Res Commun, 2014, 448(2): 212-217. DOI: 10.1016/j.bbrc.2014.04.097.
4. 段红涛, 陈松.间充质干细胞在视网膜疾病应用研究进展[J]. 中国实用眼科杂志, 2015, 33 (3): 213-216. DOI: 10.3760/cma.j.issn.1006-4443.2015.03.001.Duan HT, Cheng S.Progress in the application of mesenchymal stem cells in retinal diseases[J]. Chin J Pract Ophthalmol, 2015, 33 (3): 213-216.DOI: 10.3760/cma.j.issn.1006-4443.2015.03.001.
5. Tolar J, Nauta AJ, Osborn MJ, et al. Sarcoma derived from cultured mesenchymal stem cells[J]. Stem Cells, 2007, 25(2): 371-379. DOI: 10.1634/stemcells.2005-0620.
6. Yi T, Song SU. Immunomodulatory properties of mesenchymal stem cells and their therapeutic applications[J]. Arch Pharm Res, 2012, 35(2): 213-221. DOI: 10.1007/s12272-012-0202-z.
7. Galipeau J. The mesenchymal stromal cells dilemma--does a negative phase Ⅲtrial of random donor mesenchymal stromal cells in steroid-resistant graft-versus-host disease represent a death knell or a bump in the road?[J]. Cytotherapy, 2013, 15(1): 2-8. DOI: 10.1016/j.jcyt.2012.10.002.
8. 王健, 陈松.基质细胞衍生因子-1/CXC趋化因子受体4通路在间充质干细胞治疗糖尿病视网膜病变中的作用研究[J]. 中华眼底病杂志, 2016, 32(6): 663-667. DOI: 10.3760/cma.j.issn.1005-1015.2016.06.029.Wang J, Chen S. Role of stromal cell derived factor-1/CXC chemokine receptor 4 pathway in mesenchymal stem cells therapies in the management of diabetic retinopathy[J]. Chin J Ocul Fundus Dis, 2016, 32(6): 663-667.DOI: 10.3760/cma.j.issn.1005-1015.2016.06.029.
9. 宋雷, 杨跃进, 董秋婷, 等. 阿托伐他汀主要经AMP蛋白激酶而非PI3K/Akt信号通路抑制猪骨髓间充质干细胞凋亡[J]. 中华心血管病杂志, 2011, 39(11): 1033-1038. DOI: 10.3760/cma.j.issn.0253-3758.2011.11.013.Song L, Yang YJ, Dong QT, et al. Atorvastatin protects swine bone marrow mesenchymal stem cells from apoptosis through AMPK but not PI3K/Akt pathway[J]. Chin J Cardiol, 2011, 39(11): 1033-1038. DOI: 10.3760/cma.j.issn.0253-3758.2011.11.013.
10. Zhang Y, Wittner M, Bouamar H, et al. Identification of CXCR4 as a new nitric oxide-regulated gene in human CD34+cells[J]. Stem Cells, 2007, 25(1): 211-219. DOI: 10.1634/stemcells.2006-0468.
11. Yamagishi S, Matsui T. Advanced glycation end products(AGEs), oxidative stress and diabetic retinopathy[J]. Cnit Pharm Biotechnol, 2011, 12(3): 362-368.
12. Lee JK, Park SR, Jung BK, et al. Exosomes derived from mesenchymal stem cells suppress angiogenesis by down-regulating VEGF expression in breast cancer cells[J/OL]. PLoS One, 2013, 8(12): 84256[2013-12-31]. http://dx.plos.org/1al.pone.0084256.
13. Lai RC, Yeo RW, Padmanabhan J, et al. Isolation andcharacterization of exosome from human embryonic stem cellderived C-myc-immortalized mesenchymal stem cells[J]. Methods Mol Biol, 2016, 1416: 477-494.DOI: 10.1007/978-1-4939-3584-0_29.
14. Nakano M, Nagaishi K, Konari N, et al. Bone marrow. derivedmesenchymal stem ceils improve diabetes-induced cognitiveimpairment by exosome transfer into damaged neurons and astrocytes [J]. Sci Rep, 2016, 22(6): 24805.DOI: 10.1038/srep24805.
15. Boomsma RA, Geenen DL.Mesenchymal stem cells secrete multiple cytokines that promote angiogenesis and have contrasting effects on chemotaxis and apoptosis[J/OL]. PLoS One, 2012, 7 (4): 35685[2012-04-25]. https://doi.org/10.1371/journal.pone.0035685. DOI: 10.1371/journal.pone.0035685.
16. Xu X, Zhu F, Zhang M, et al.Stromal cell-derived factor-1 enhances wound healing through recruiting bone marrow-derived mesenchymal stem cells to the wound area and promoting neovascularization[J]. Cells Tissues Organs, 2013, 197(2): 103-113. DOI: 10.1159/000342921.
17. Bolli R, Dawn B. The cornucopia of " pleiotropic” actions of statins: myogenesis as a new mechanism for statin-induced benefits[J]. Circ Res, 2009, 104(2): 144-146. DOI: 10.1161/CIRCRESAHA.108.192500.
18. Nakanishi C, Yamagishi M, Yamahara K, et al. Activation of cardiac progenitor cells through paracrine effects of mesenchymal stem cells[J]. Biochem Biophys Res Commun, 2008, 374 (1): 11-16. DOI: 10.1016/j.bbrc.2008.06.074.
19. Li N, Yang YJ, Qian HY, et al. Intravenous administration of atorvastatin-pretreated mesenchymal stem cells improves cardiac performance after acute myocardial infarction: role of CXCR4[J]. Am J Transl Res, 2015, 7(6): 1058-1070.
20. 张惟, 韩琪, 陈松, 等.辛伐他汀对糖尿病大鼠骨髓内皮祖细胞数量和功能的影响[J]. 中华眼科杂志, 2012, 48(11): 1015-1020.DOI: 10.3760/cma.j.issn.0412-4081.2012.11.013.Zhang.Zhang W, Han Q, Chen S, et al. Effect of simvastatin on the amount and function of endothelial progenitor cells from bone marrow in diabetic rats[J]. Chin J Ophthalmol, 2012, 48(11): 1015-1020. DOI: 10.3760/cma.j.issn.0412-4081.2012.11.013.

Chinese Journal of Ocular Fundus Diseases

Effect of atorvastatin on the expression of chemokine receptor-4 and migration ability of rat bone marrow mesenchymal stem cells cultivated in vitro

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