RESEARCH ADVANCE OF DIFFERENTIATION OF INDUCED PLURIPOTENT STEM CELLS INTO Schwann CELLS IN VITRO_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LIRunxin , LIUHuawei , YANRongzeng ,  HUMin

Department of Oral and Maxillofacial Surgery, General Hospital of Chinese PLA, Beijing, 100853, P. R. China;

Corresponding author：

HUMin, Email: humin48@vip.163.com

Keywords：

Induced pluripotent stem cells; Neural crest; Schwann cells; Differentiation; Identification

DOI：

10.7507/1002-1892.20150332

Video：

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Objective To review the research advance of differentiation of induced pluripotent stem cells (iPS) into Schwann cells in vitro in recent years. Methods Related literatures on differentiation of iPS into Schwann cells in vitro at present were consulted, the induction methods of iPS differentiating into Schwann cells in vitro were summarized, and the differentiated cells were identified and detected. Results The research results indicate that iPS can differentiate into Schwann cells. So far, the iPS have to differentiate into neural crest cells or neural crest stem cells firstly, and then differentiate into Schwann cells. S100-β and glial fibrillary acidic protein (GFAP) are recognized as the marker of Schwann cells. The evidence of generating Schwann cells was that the neural crest cells or neural crest stem cells were labelled by p75⁺, HNK1⁺, or nestin⁺ before differentiation, and by S100-β⁺ and GFAP⁺ after induction. Conclusion Despite the increasing reported studies of Schwann cells from iPS, there have been few successful induction methods, so this field of cytology needs further study.

Citation： LIRunxin, LIUHuawei, YANRongzeng, HUMin. RESEARCH ADVANCE OF DIFFERENTIATION OF INDUCED PLURIPOTENT STEM CELLS INTO Schwann CELLS IN VITRO. Chinese Journal of Reparative and Reconstructive Surgery, 2015, 29(12): 1560-1563. doi: 10.7507/1002-1892.20150332 Copy

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15.	Arthur-Farraj P, Wanek K, Hantke J, et al. Mouse schwann cells need both NRG1 and cyclic AMP to myelinate. Glia, 2011, 59(5):720-733.
16.	Adlkofer K, Lai C. Role of neuregulins in glial cell development. Glia, 2000, 29(2):104-111.
17.	Nave KA, Salzer JL. Axonal regulation of myelination by neuregulin 1. Curr Opin Neurobiol, 2006, 16(5):492-500.
18.	Kao SC, Wu H, Xie J, et al. Calcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Science, 2009, 323(5914):651-654.
19.	Liu Q, Spusta SC, Mi R, et al. Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells:induction, maintenance, and differentiation into functional schwann cells. Stem Cells Transl Med, 2012, 1(4):266-278.
20.	Lee G, Chamber SM, Tomishima MJ, et al. Derivation of neural crest cells from human pluripotent stem cells. Nat Protoc, 2010, 5(4):688-701.
21.	Wang A, Tang Z, Park IH, et al. Induced pluripotent stem cells for neural tissue engineering. Biomaterials, 2011, 32(22):5023-5032.
22.	Liu Q, Swistowski A, Zeng X. Human neural crest stem cells derived from human pluripotent stem cells. Methods Mol Biol, 2014, 1210:79-90.
23.	Menendez L, Kulik MJ, Page AT, et al. Directed differentiation of human pluripotent cells to neural crest stem cells. Nat Protoc, 2013, 8(1):203-212.
24.	Ma MS, Boddeke E, Copray S. Pluripotent stem cells for Schwann cell engineering. Stem Cell Rev, 2015, 11(2):205-218.
25.	吴蔚蔚. 噬菌体库筛选猕猴神经干细胞表面标记物. 北京:清华大学, 2010.
26.	秦煜, 顾立强, 董文其. 感觉和运动神经来源的雪旺细胞p75受体表达的研究. 中华实验外科杂志, 2000, 17(2):118-119.
27.	林婉颐, 黄利华, 周立文, 等. 人诱导多能干细胞向外周神经元分化的实验研究. 中山大学学报(医学科学版), 2014, 35(3):431-437.

1. Webber C, Zochodne D. The nerve regenerative microenvironment:early behavior and partnership of axons and Schwann cells. Exp Neurol, 2010, 223(1):51-59.
2. Fairbairn NG, Meppelink AM, Ng-Glazier J, et al. Augmenting peripheral nerve regeneration using stem cells:A review of current opinion. World J Stem Cells, 2015, 7(1):11-26.
3. Tang YD, Zheng XS, Ying TT, et al. Nimodipine-mediated remyelination after facial nerve crush injury in rats. J Clin Neurosci, 2015, 22(10):1661-1668.
4. Saheb-Al-Zamani M, Yan Y, Farber SJ, et al. Limited regeneration in long acellular nerve allografts is associated with increased Schwann cell senescence. Exp Neurol, 2013, 247:165-177.
5. 文勇, 徐欣, 马跃, 等. 聚四氟乙烯膜管内植入许旺细胞与神经生长因子桥接面神经的比较. 中国组织工程研究与临床康复, 2008, 12(49):9631-9634.
6. Mirsky R, Woodhoo A, Parkinson DB, et al. Novel signals controlling embryonic Schwann cell development, myelination and dedifferentiation. J Peripher Nerv Syst, 2008, 13(2):122-135.
7. Bronner ME, LeDouarin NM. Development and evolution of the neural crest:an overview. Dev Biol, 2012, 366(1):2-9.
8. Trainor PA. Specification of neural crest cell formation and migration in mouse embryos. Semin Cell Dev Biol, 2005, 16(6):683-693.
9. Raible DW, Ragland JW. Reiterated Wnt and BMP signals in neural crest development. Semin Cell Dev Biol, 2005, 16(6):673-682.
10. Kalluri R, Weinberg RA. The basics of epithelial-mesenchymal transition. J Clin Invest, 2009, 119(6):1420-1428.
11. Dupin E, Sommer L. Neural crest progenitors and stem cells:from early development to adulthood. Dev Biol, 2012, 366(1):83-95.
12. Finzsch M, Schreiner S, Kichko T, et al. Sox10 is required for Schwann cell identity and progression beyond the immature Schwann cell stage. J Cell Biol, 2010, 189(4):701-712.
13. Ghislain J, Desmarquet-Trin-Dinh C, Jaegle M, e t al. Characterisation of cis-acting sequences reveals a biphasic, axondependent regulation of Krox20 during Schwann cell development. Development, 2002, 129(1):155-166.
14. Britsch S, Goerich DE, Riethmacher D, et al. The transcription factor Sox10 is a key regulator of peripheral glial development. Genes Dev, 2001, 15(1):66-78.
15. Arthur-Farraj P, Wanek K, Hantke J, et al. Mouse schwann cells need both NRG1 and cyclic AMP to myelinate. Glia, 2011, 59(5):720-733.
16. Adlkofer K, Lai C. Role of neuregulins in glial cell development. Glia, 2000, 29(2):104-111.
17. Nave KA, Salzer JL. Axonal regulation of myelination by neuregulin 1. Curr Opin Neurobiol, 2006, 16(5):492-500.
18. Kao SC, Wu H, Xie J, et al. Calcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Science, 2009, 323(5914):651-654.
19. Liu Q, Spusta SC, Mi R, et al. Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells:induction, maintenance, and differentiation into functional schwann cells. Stem Cells Transl Med, 2012, 1(4):266-278.
20. Lee G, Chamber SM, Tomishima MJ, et al. Derivation of neural crest cells from human pluripotent stem cells. Nat Protoc, 2010, 5(4):688-701.
21. Wang A, Tang Z, Park IH, et al. Induced pluripotent stem cells for neural tissue engineering. Biomaterials, 2011, 32(22):5023-5032.
22. Liu Q, Swistowski A, Zeng X. Human neural crest stem cells derived from human pluripotent stem cells. Methods Mol Biol, 2014, 1210:79-90.
23. Menendez L, Kulik MJ, Page AT, et al. Directed differentiation of human pluripotent cells to neural crest stem cells. Nat Protoc, 2013, 8(1):203-212.
24. Ma MS, Boddeke E, Copray S. Pluripotent stem cells for Schwann cell engineering. Stem Cell Rev, 2015, 11(2):205-218.
25. 吴蔚蔚. 噬菌体库筛选猕猴神经干细胞表面标记物. 北京:清华大学, 2010.
26. 秦煜, 顾立强, 董文其. 感觉和运动神经来源的雪旺细胞p75受体表达的研究. 中华实验外科杂志, 2000, 17(2):118-119.
27. 林婉颐, 黄利华, 周立文, 等. 人诱导多能干细胞向外周神经元分化的实验研究. 中山大学学报(医学科学版), 2014, 35(3):431-437.

Chinese Journal of Reparative and Reconstructive Surgery

RESEARCH ADVANCE OF DIFFERENTIATION OF INDUCED PLURIPOTENT STEM CELLS INTO Schwann CELLS IN VITRO

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