Improvement of Method for Primary Culture of Vascular Smooth Muscle Cells of Diabetic Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YANGTing , JINSong ,  LIUYan-dong

Department of Vascular Surgery, The First Hospital Affiliated to Jiamusi University, Jiamusi 154000, Heilongjiang Province, China;

Corresponding author：

LIUYan-dong, Email: liuyandong867719@126.com

Keywords：

Diabetes rat; Aortic vascular smooth muscle cell; Cultured in vitro

DOI：

10.7507/1007-9424.20140040

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore a simple, efficient method for primary culture in vascular smooth muscle cells of diabetic rat, to establish a long-term stable diabetic vascular smooth muscle cell model in vitro, and lay the foundation for the study of diabetes chronic vascular lesions. Methods Twenty diabetic Wister rats models were self-made by using streptozotocin intraperitoneal injection plus high fat and sugar feeding, the vascular smooth muscle cells in vitro was cultured by a modified enzymatic digestion. Results The diabetic rat models were successfully established, the vascular smooth muscle cells cultured in vitro by modified enzyme digestion grew fast, the cell survival was 96%, it could meet the requirements for cell experiment in vitro. Conclusion Comparing with the traditional method, the modified enzymatic digestion method is simple, economic, high survival rate.

Citation： YANGTing, JINSong, LIUYan-dong. Improvement of Method for Primary Culture of Vascular Smooth Muscle Cells of Diabetic Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(2): 173-176. doi: 10.7507/1007-9424.20140040 Copy

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17.	胡向阳, 黄晓明, 刘乃丰, 等.高度糖基化终产物对大鼠主动脉平滑肌细胞G-蛋白亚基基因表达的影响[J].东南大学学报:医学版, 2004, 23(3):145-147.
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19.	卢圣栋.现代分子生物学实验技术[M].第2版.北京:中国协和医科大学出版社, 1999:439-441.

1. 李萍, 郑莲星, 王伶, 等.反复贴块法培养大鼠主动脉平滑肌细胞[J].中国组织工程研究与临床康复, 2007, 11(46):9319-9321.
2. 毛昱嘉, 王文杰.胰酶消化法培养大鼠胸主动脉平滑肌细胞[J].中国动脉硬化杂志, 2004, 12(6):729-731.
3. 王忠太, 马佳玲.组织块贴壁法培养大鼠主动脉平滑肌细胞及鉴定[J].卫生职业教育, 2010, 28(3):97-98.
4. 姚飞.常用的细胞培养方法、污染及污染处理[J].科技信息, 2009, (7):422, 447.
5. 王立新, 杨朝霞.动物细胞培养及应用[J].黄牛杂志, 2000, 26(4):45-48.
6. 司徒镇强.细胞培养[M].第2版.北京:世界图书出版社, 2007:196.
7. 司徒镇强.细胞培养[M].第2版.北京:世界图书出版社, 2007:222-223.
8. Laakso M.Hyperglycemia and cardiovascular disease in type 2 diabetes[J].Diabetes, 1999, 48(5):937-942.
9. Ross R.The pathogenesis of atherosclerosis:a perspective for the 1990s[J].Nature, 1993, 362(6423):801-809.
10. Overbeck HW, Wu G, Durham JP.Humoral factor (s) in hypertensive rats trophic for cultured aortic smooth muscle cells[J].Am J Physiol, 1995, 268(2 Pt 2):767-772.
11. 倪伟, 刘涛, 王浩宇, 等.酶联合消化法培养小鼠主动脉平滑肌原代细胞[J].西部医学, 2013, 25(7):968-969.
12. 辛毅, 许秀芳, 黄益民, 等.乳小鼠心肌成纤维细胞和心肌细胞的分离培养及荧光鉴定[J].新乡医学院学报, 2011, 28(5):541-547.
13. 杨东伟, 张清华, 蒋知新, 等.大鼠胸主动脉平滑肌细胞胶原酶解法培养与鉴定[J].中国现代医学杂志, 2009, 19(9):1333-1335.
14. 涂永生, 黄红林, 朱炳阳, 等.Ⅱ型胶原酶/弹性蛋白酶消化法培养大鼠血管平滑肌细胞[J].中国动脉硬化杂志, 2001, 9(5):438-440.
15. 张鹏, 林福玉, 张迎梅, 等.小鼠主动脉血管平滑肌原代细胞的分离培养及鉴定[J].生物技术通讯, 2010, 21(1):54-57, 79.
16. 严利君, 谢自敬, 樊勇.高血糖对实验性糖尿病大鼠心脏血管病变的作用[J].武警医学, 2004, 15(4):266-269.
17. 胡向阳, 黄晓明, 刘乃丰, 等.高度糖基化终产物对大鼠主动脉平滑肌细胞G-蛋白亚基基因表达的影响[J].东南大学学报:医学版, 2004, 23(3):145-147.
18. 司徒镇强.细胞培养[M].第2版.北京:世界图书出版社, 2007:54-57.
19. 卢圣栋.现代分子生物学实验技术[M].第2版.北京:中国协和医科大学出版社, 1999:439-441.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Improvement of Method for Primary Culture of Vascular Smooth Muscle Cells of Diabetic Rats

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