Effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 ZHANG Panpan , HAN Xiaoqing , WANG Yuan , HUANG Chao , WANG Hongyang

Department of Respiratory Medicine, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei 063000, P. R. China;

Corresponding author：

ZHANG Panpan, Email: 1983zhangpanpan@163.com

Keywords：

Intermittent hypoxia; total glycosides of Cistanche deserticola; oxidative stress; cognitive function

DOI：

10.7507/1671-6205.202212001

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Objective To explore the effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia. Methods Adult male Wistar rats (n=72) were randomly divided into three groups: a blank control group, a 5% intermittent hypoxia group (IH group) and a total glucosides of Cistanche deserticola intervention group (TGs intervention group). The 5% intermittent hypoxia rat model was simulated by using the self-made cabin of intermittent hypoxia. The rats in the IH group and the TGs intervention group were given 5% intermittent hypoxia respectively, and the rats in the TGs intervention group were treated with total glucosides of Cistanche deserticola simultaneously. Learning and memory function was tested by Morris water maze in three groups at the 2^nd, 4^th, 6^th and 8^th week respectively. The expressions of superoxide dismutase (SOD) and malondialdehyde (MDA) in hippocampus were detected by test kit. Results Compared with the blank control group, the escape latency time of the rats in the IH group and the TGs intervention group was significantly prolonged at the 2^nd, 4^th, 6^th and 8^th week respectively (P<0.05). The time to cross the target quadrant in the IH group and the TGs intervention group was gradually shortened at the 2^nd, 4^th, 6^th and 8^th week respectively (P<0.05). Compared with the IH group, the escape latency gradually shortened at the 2^nd, 4^th, 6^th and 8^th week in the TGs intervention group (P<0.05), while the time to cross the target quadrant was gradually prolonged at the 2^nd, 4^th, 6^th and 8^th week (P<0.05). The expressions of MDA in hippocampal tissue in the IH group and the TGs intervention group increased at the 2^nd, 4^th, 6^th and 8^th week (P<0.05), which were significantly higher than those in the blank control group; and the expressions of SOD at the 2^nd, 4^th, 6^th, and 8^th week were all lower than those in the blank control group(P<0.05). Compared with the IH group, the expression of MDA protein in hippocampal tissue in the TGs intervention group decreased at the 2^nd, 4^th, 6_th and 8^th week, while the expression of SOD protein at the 2^nd, 4^th, 6^th and 8^th week increased, and the differences were statistically significant (P<0.05). Conclusion The total glycosides of Cistanche deserticola can improve the learning and memory function of intermittent hypoxia rats by inhibiting oxidative stress.

Citation： ZHANG Panpan, HAN Xiaoqing, WANG Yuan, HUANG Chao, WANG Hongyang. Effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia. Chinese Journal of Respiratory and Critical Care Medicine, 2023, 22(4): 269-273. doi: 10.7507/1671-6205.202212001 Copy

1.	张盼盼, 汪彦辉, 韩晓庆, 等. 丁苯酞对老年OSAHS患者氧化应激及认知功能的影响. 临床耳鼻咽喉头颈外科杂志, 2018, 32(18): 1422-1425.
2.	王乐, 张静, 王彦, 等. 整夜睡眠血氧饱和度低于90%时间占总监测时间的百分比与阻塞性睡眠呼吸暂停低通气常见合并症的关系. 中华结核和呼吸杂志, 2020, 43(1): 63-67.
3.	Vanek J, Prasko J, Genzor S, et al. Obstructive sleep apnea, depression and cognitive impairment. Sleep Med, 2020, 72: 50-58.
4.	Xu LH, Yang YB, Chen J. The role of reactive oxygen species in cognitive impairment associated with sleep apnea. Exp Ther Med, 2020, 20(5): 4.
5.	Zhou L, Chen P, Peng YT, et al. Role of oxidative stress in the neurocognitive dysfunction of obstructive sleep apnea syndrome. Oxid Med Cell Longev, 2016, 2016: 9626831.
6.	董文平, 任寿安. 血清核转录因子红系2相关因子2、血红素加氧酶-1水平与阻塞性睡眠呼吸暂停患者认知障碍的相关性研究. 中国呼吸与危重监护杂志, 2022, 21(7): 478-483.
7.	韩思璐, 马迎民. 阻塞性睡眠呼吸暂停患者持续气道正压通气治疗相关吞气症和胃食管反流. 中国呼吸与危重监护杂志, 2018, 17(6): 639-643.
8.	Jia JX, Yan XS, Cai ZP, et al. The effects of phenylethanoid glycosides, derived from Herba cistanche, on cognitive deficits and antioxidant activities in male SAMP8 mice. J Toxicol Environ Health A, 2017, 80(22): 1180-1186.
9.	Yang JH, Ju BW, Hu JP. Effects of phenylethanoid glycosides extracted from Herba cistanches on the learning and memory of the APP/PSI transgenic mice with Alzheimer's disease. Biomed Res Int, 2021, 2021: 1291549.
10.	王璐, 白雨朦, 李晓宇, 等. 肉苁蓉总苷对阿尔茨海默病模型大鼠学习认知功能和氧化应激的影响. 解剖学杂志, 2020, 43(3): 194-199.
11.	王红阳, 韩晓庆, 赵雅宁, 等. 重度间歇低氧大鼠学习记忆功能与氧化应激的关系. 中华结核和呼吸杂志, 2012, 35(1): 24-28.
12.	张盼盼, 韩晓庆, 王红阳, 等. 间歇低氧大鼠神经元特异性烯醇化酶蛋白的表达及其对学习记忆功能的影响. 中华行为医学与脑科学杂志, 2014, 23(4): 303-306.
13.	Patel A, Chong DJ. Obstructive sleep apnea: cognitive outcomes. Clin Geriatr Med, 2021, 37(3): 457-467.
14.	Tóthová Ľ, Celec P, Mucska I, et al. Short-term effects of continuous positive airway pressure on oxidative stress in severe sleep apnea. Sleep Breath, 2019, 23(3): 857-863.
15.	马晓蓉, 张静宜, 宗运之, 等. 无创呼吸机治疗对阻塞性睡眠呼吸暂停低通气综合征患者骨代谢和氧化应激影响的研究. 中国呼吸与危重监护杂志, 2022, 21(10): 731-736.
16.	Rosenzweig I, Glasser M, Crum WR, et al. Changes in neurocognitive architecture in patients with obstructive sleep apnea treated with continuous positive airway pressure. EBioMedicine, 2016, 7: 221-229.
17.	韩晓庆, 张盼盼, 李琳, 等. 依达拉奉对重度间歇低氧大鼠认知功能及Nogo-A表达的影响. 中国中西医结合急救杂志, 2017, 24(6): 570-574.
18.	Abdel-Wahab BA, Abdel-Wahab MM. Protective effect of resveratrol against chronic intermittent hypoxia-induced spatial memory deficits, hippocampaloxidative DNA damage and increased p47Phox NADPH oxidase expression in young rats. Behav Brain Res, 2016, 305: 65-75.
19.	Wang FJ, Li RY, Tu PF, et al. Total glycosides of Cistanche deserticola promote neurological function recovery by inducing neurovascular regeneration via Nrf-2/Keap-1 pathway in MCAO/R rats. Front Pharmacol, 2020, 11: 236.
20.	薛海燕, 焦婵媛, 姚军. 肉苁蓉总苷药理作用的研究现状. 中国临床药理学杂志, 2018, 34(4): 486-488.
21.	Ling JZ, Yu Q, Li YN, et al. Edaravone improves intermittent hypoxia-induced cognitive impairment and hippocampal damage in rats. Biol Pharm Bull, 2020, 43(8): 1196-1201.
22.	Elliot-Portal E, Laouafa S, Arias-Reyes C, et al. Brain-derived erythropoietin protects from intermittent hypoxia-induced cardiorespiratory dysfunction and oxidative stress in mice. Sleep, 2018, 41(7): zsy072.
23.	樊燕燕, 张石在. 肉苁蓉总苷通过调控lncRNA GAS5保护神经细胞缺血再灌注损伤. 中成药, 2022, 44(7): 2320-2324.
24.	刘恩宠, 田原, 韦瑶, 等. 肉苁蓉毛蕊花苷改善脑低灌注致小鼠认知功能障碍. 烟台大学学报(自然科学与工程版), 2022, 35(2): 170-175.
25.	Peng XM, Gao L, Huo SX, et al. The mechanism of memory enhancement of acteoside (verbascoside) in the senescent mouse model induced by a combination of D-gal and AlCl₃. Phytother Res, 2015, 29(8): 1137-1144.
26.	黄惠莲, 陈莉. 肉苁蓉总苷对血管性痴呆病人认知功能和血清p-tau蛋白表达的影响. 中西医结合心脑血管病杂志, 2019, 17(8): 1248-1250.
27.	刘心朗, 周丽丽, 杨占君, 等. 肉苁蓉总苷对SAMP8小鼠学习记忆能力及突触可塑性的影响. 中华中医药学刊, 2022, 40(1): 110-115, 276, 277.
28.	贾建新, 宋嵬, 闫旭升, 等. 肉苁蓉总苷对SAMP8小鼠空间学习记忆的影响及其机制. 包头医学院学报, 2014, 30(6): 6-8.

1. 张盼盼, 汪彦辉, 韩晓庆, 等. 丁苯酞对老年OSAHS患者氧化应激及认知功能的影响. 临床耳鼻咽喉头颈外科杂志, 2018, 32(18): 1422-1425.
2. 王乐, 张静, 王彦, 等. 整夜睡眠血氧饱和度低于90%时间占总监测时间的百分比与阻塞性睡眠呼吸暂停低通气常见合并症的关系. 中华结核和呼吸杂志, 2020, 43(1): 63-67.
3. Vanek J, Prasko J, Genzor S, et al. Obstructive sleep apnea, depression and cognitive impairment. Sleep Med, 2020, 72: 50-58.
4. Xu LH, Yang YB, Chen J. The role of reactive oxygen species in cognitive impairment associated with sleep apnea. Exp Ther Med, 2020, 20(5): 4.
5. Zhou L, Chen P, Peng YT, et al. Role of oxidative stress in the neurocognitive dysfunction of obstructive sleep apnea syndrome. Oxid Med Cell Longev, 2016, 2016: 9626831.
6. 董文平, 任寿安. 血清核转录因子红系2相关因子2、血红素加氧酶-1水平与阻塞性睡眠呼吸暂停患者认知障碍的相关性研究. 中国呼吸与危重监护杂志, 2022, 21(7): 478-483.
7. 韩思璐, 马迎民. 阻塞性睡眠呼吸暂停患者持续气道正压通气治疗相关吞气症和胃食管反流. 中国呼吸与危重监护杂志, 2018, 17(6): 639-643.
8. Jia JX, Yan XS, Cai ZP, et al. The effects of phenylethanoid glycosides, derived from Herba cistanche, on cognitive deficits and antioxidant activities in male SAMP8 mice. J Toxicol Environ Health A, 2017, 80(22): 1180-1186.
9. Yang JH, Ju BW, Hu JP. Effects of phenylethanoid glycosides extracted from Herba cistanches on the learning and memory of the APP/PSI transgenic mice with Alzheimer's disease. Biomed Res Int, 2021, 2021: 1291549.
10. 王璐, 白雨朦, 李晓宇, 等. 肉苁蓉总苷对阿尔茨海默病模型大鼠学习认知功能和氧化应激的影响. 解剖学杂志, 2020, 43(3): 194-199.
11. 王红阳, 韩晓庆, 赵雅宁, 等. 重度间歇低氧大鼠学习记忆功能与氧化应激的关系. 中华结核和呼吸杂志, 2012, 35(1): 24-28.
12. 张盼盼, 韩晓庆, 王红阳, 等. 间歇低氧大鼠神经元特异性烯醇化酶蛋白的表达及其对学习记忆功能的影响. 中华行为医学与脑科学杂志, 2014, 23(4): 303-306.
13. Patel A, Chong DJ. Obstructive sleep apnea: cognitive outcomes. Clin Geriatr Med, 2021, 37(3): 457-467.
14. Tóthová Ľ, Celec P, Mucska I, et al. Short-term effects of continuous positive airway pressure on oxidative stress in severe sleep apnea. Sleep Breath, 2019, 23(3): 857-863.
15. 马晓蓉, 张静宜, 宗运之, 等. 无创呼吸机治疗对阻塞性睡眠呼吸暂停低通气综合征患者骨代谢和氧化应激影响的研究. 中国呼吸与危重监护杂志, 2022, 21(10): 731-736.
16. Rosenzweig I, Glasser M, Crum WR, et al. Changes in neurocognitive architecture in patients with obstructive sleep apnea treated with continuous positive airway pressure. EBioMedicine, 2016, 7: 221-229.
17. 韩晓庆, 张盼盼, 李琳, 等. 依达拉奉对重度间歇低氧大鼠认知功能及Nogo-A表达的影响. 中国中西医结合急救杂志, 2017, 24(6): 570-574.
18. Abdel-Wahab BA, Abdel-Wahab MM. Protective effect of resveratrol against chronic intermittent hypoxia-induced spatial memory deficits, hippocampaloxidative DNA damage and increased p47Phox NADPH oxidase expression in young rats. Behav Brain Res, 2016, 305: 65-75.
19. Wang FJ, Li RY, Tu PF, et al. Total glycosides of Cistanche deserticola promote neurological function recovery by inducing neurovascular regeneration via Nrf-2/Keap-1 pathway in MCAO/R rats. Front Pharmacol, 2020, 11: 236.
20. 薛海燕, 焦婵媛, 姚军. 肉苁蓉总苷药理作用的研究现状. 中国临床药理学杂志, 2018, 34(4): 486-488.
21. Ling JZ, Yu Q, Li YN, et al. Edaravone improves intermittent hypoxia-induced cognitive impairment and hippocampal damage in rats. Biol Pharm Bull, 2020, 43(8): 1196-1201.
22. Elliot-Portal E, Laouafa S, Arias-Reyes C, et al. Brain-derived erythropoietin protects from intermittent hypoxia-induced cardiorespiratory dysfunction and oxidative stress in mice. Sleep, 2018, 41(7): zsy072.
23. 樊燕燕, 张石在. 肉苁蓉总苷通过调控lncRNA GAS5保护神经细胞缺血再灌注损伤. 中成药, 2022, 44(7): 2320-2324.
24. 刘恩宠, 田原, 韦瑶, 等. 肉苁蓉毛蕊花苷改善脑低灌注致小鼠认知功能障碍. 烟台大学学报(自然科学与工程版), 2022, 35(2): 170-175.
25. Peng XM, Gao L, Huo SX, et al. The mechanism of memory enhancement of acteoside (verbascoside) in the senescent mouse model induced by a combination of D-gal and AlCl₃. Phytother Res, 2015, 29(8): 1137-1144.
26. 黄惠莲, 陈莉. 肉苁蓉总苷对血管性痴呆病人认知功能和血清p-tau蛋白表达的影响. 中西医结合心脑血管病杂志, 2019, 17(8): 1248-1250.
27. 刘心朗, 周丽丽, 杨占君, 等. 肉苁蓉总苷对SAMP8小鼠学习记忆能力及突触可塑性的影响. 中华中医药学刊, 2022, 40(1): 110-115, 276, 277.
28. 贾建新, 宋嵬, 闫旭升, 等. 肉苁蓉总苷对SAMP8小鼠空间学习记忆的影响及其机制. 包头医学院学报, 2014, 30(6): 6-8.

Chinese Journal of Respiratory and Critical Care Medicine

Effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia

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