Analysis of iron acquisition and transportation system in clinical <i>Klebsiella pneumoniae</i> isolates _Chinese Journal of Evidence-Based Medicine

Authors：

ZHANG Jie , HUANG Xiangning , LIU Xin ,  YU Hua

Department of Clinical Laboratory Medicine, Sichuan Academy of Medical Sciences; Sichuan Provincial People's Hospital, Chengdu, 610072, P.R.China;

Corresponding author：

YU Hua, Email: yvhua2002@163.com

Keywords：

Klebsiella pneumoniae; Iron system gene; Next generation sequencing; Pathogenicity

DOI：

10.7507/1672-2531.201801039

Video：

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ObjectiveTo evaluate the relationship between the genes involved in regulating iron uptake and maintaining iron homeostasis in Klebsiella pneumoniae from different sources and pathogenicity.MethodsThe genomic DNA sequences of two strains of Klebsiella pneumoniae from different sources were sequenced, stitched together, annotated and analyzed by second generation sequencing technique. The transversal comparison between different types of Klebsiella pneumoniae in NCBI database of iron carrier gene cluster iroB/C/D.ResultsIn these two Klebsiella pneumoniae clinical strains, the strain isolated from liver abscess patient carried 11 different iron acquisition and transportation system specific genes, which were not found in the non-liver abscess patient strain. Combined with the analysis of this sequence, in the NCBI database, six different strains of Klebsiella pneumoniae showed iroB/C/D triple positive.ConclusionIron acquisition and transportation system in Klebsiella pneumoniae may be an important pathogenic factor, which is closely related to hepatic abscess.

Citation： ZHANG Jie, HUANG Xiangning, LIU Xin, YU Hua. Analysis of iron acquisition and transportation system in clinical Klebsiella pneumoniae isolates . Chinese Journal of Evidence-Based Medicine, 2018, 18(3): 276-279. doi: 10.7507/1672-2531.201801039 Copy

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8.	尹大龙, 刘连新. 细菌性肝脓肿诊治进展. 中国实用外科杂志, 2013, 33(9): 793-795.
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10.	吴华, 李东冬, 王京, 等. 肺炎克雷伯菌肝脓肿的临床及微生物特征分析. 中华医学杂志, 2015, 95(40): 3259-3263.
11.	汪强, 葛玉梅, 刘建芳, 等. 肝脓肿高毒力肺炎克雷伯菌耐药性及毒力基因型流行性分析. 中华医院感染学杂志, 2016, 26(24): 5531-5533, 5586.
12.	马荣, 刘科芳, 聂大平, 等. 肺炎克雷伯菌中 K1、K2 血清型菌株的分布及其耐药性分析. 中国感染与化疗杂志, 2015, 15(3): 204-208.
13.	和晋渝. 肺炎克雷伯菌的血清分型及毒力基因分布的研究. 重庆: 重庆医科大学, 2012.
14.	陈杨, 刘嘉琳, 瞿洪平. 铁载体对肺炎克雷伯菌毒力增强的机制研究. 中国感染与化疗杂志, 2016, 16(6): 804-807.
15.	Ye M, Tu J, Jiang J, et al. Clinical and genomic analysis of liver abscess-causing klebsiella pneumoniae identifies new liver abscess-associated virulence genes. Front Cell Infect Microbiol, 2016, 6: 165.

1. Liu Y, Wang JY, Jiang W. An increasing prominent disease of Klebsiella pneumoniae liver abscess: etiology, diagnosis, and treatment. Gastroenterol Res Pract, 2013, 2013: 258514.
2. Lin YC, Lu MC, Tang HL, et al. Assessment of hypermucoviscosity as a virulence factor for experimental Klebsiella pneumoniae infections: comparative virulence analysis with hypermucoviscosity-negative strain. BMC Microbiol, 2011, 11: 50.
3. Nairz M, Haschka D, Demetz E, et al. Iron at the interface of immunity and infection. Front Pharmacol, 2014, 5: 152.
4. Cassat JE, Skaar EP. Iron in infection and immunity. Cell Host Microbe, 2013, 13(5): 509-519.
5. 张杰, 喻华. 四川省人民医院肝脓肿患者血培养分离肺炎克雷伯杆菌铁系统基因携带情况的分析. 中国循证医学杂志, 2017, 17(8): 907-909.
6. Russo TA, Olson R, Macdonald U, et al. Aerobactin mediates virulence and accounts for increased siderophore production under iron-limiting conditions by hypervirulent (hypermucoviscous) Klebsiella pneumoniae. Infect Immun, 2014, 82(6): 2356-2367.
7. Holt KE, Wertheim H, Zadoks RN, et al. Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health. Proc Natl Acad Sci USA, 2015, 112(27): E3574-3581.
8. 尹大龙, 刘连新. 细菌性肝脓肿诊治进展. 中国实用外科杂志, 2013, 33(9): 793-795.
9. 陈帆, 张艳亭, 乔慧捷, 等. 肺炎克雷伯菌肝脓肿的临床特征分析. 临床肝胆病杂志, 2016, 32(4): 764-768.
10. 吴华, 李东冬, 王京, 等. 肺炎克雷伯菌肝脓肿的临床及微生物特征分析. 中华医学杂志, 2015, 95(40): 3259-3263.
11. 汪强, 葛玉梅, 刘建芳, 等. 肝脓肿高毒力肺炎克雷伯菌耐药性及毒力基因型流行性分析. 中华医院感染学杂志, 2016, 26(24): 5531-5533, 5586.
12. 马荣, 刘科芳, 聂大平, 等. 肺炎克雷伯菌中 K1、K2 血清型菌株的分布及其耐药性分析. 中国感染与化疗杂志, 2015, 15(3): 204-208.
13. 和晋渝. 肺炎克雷伯菌的血清分型及毒力基因分布的研究. 重庆: 重庆医科大学, 2012.
14. 陈杨, 刘嘉琳, 瞿洪平. 铁载体对肺炎克雷伯菌毒力增强的机制研究. 中国感染与化疗杂志, 2016, 16(6): 804-807.
15. Ye M, Tu J, Jiang J, et al. Clinical and genomic analysis of liver abscess-causing klebsiella pneumoniae identifies new liver abscess-associated virulence genes. Front Cell Infect Microbiol, 2016, 6: 165.

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