Anti-Colon Cancer Immunity of Whole Cell Lysates Pulsed Dendritic Cell_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XUE Gang , CAO Yongkuan , WANG Peihong , ZHANG Guohu , DAI Ruiwu , GONG Jiaqing , TIAN Fuzhou

* Department of General Surgery, Chengdu Army General Hospital, Chengdu 610083, ChinaCorresponding Author: XUE Gang， E-mail: kpardan@163.com;

Corresponding author：

Keywords：

Colon cancer; Dendritic cell; Immunotherapy

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Objective To detect the anti-colon cancer ability of whole cell lysates pulsed dendritic cell (DC) which acts as an adjuvant. Methods Whole cell lysates of LoVo cells were extracted with freeze thawing method, then the monocyte-derived DC were pulsed with this cellular antigen. Subsequently, the capability of antigen pulsed DC to promote T lymphocytes proliferation and the ability of T lymphocytes to kill LoVo cells were detected by 3H-TdR incorporation and lactate dehydrogenase release methods, respectively. Results The whole cell lysates of LoVo cells pulsed DC significantly stimulated T cells proliferation, and the cytotoxicity abilities of primed T cells to kill LoVo cells were also enhanced. Conclusion Tumor cell lysates which act as the cellular antigen to pulse DC can improve the efficacy of anti-cancer immune response， which provides us an experimental evidence for cancer immunotherapy.

Citation： XUE Gang,CAO Yongkuan,WANG Peihong,ZHANG Guohu,DAI Ruiwu,GONG Jiaqing,TIAN Fuzhou,.. Anti-Colon Cancer Immunity of Whole Cell Lysates Pulsed Dendritic Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(1): 48-51. doi: Copy

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1. 1 谭广，任金帅，王忠裕，等. TNF-α增强树突状细胞诱导的抗胰腺癌免疫应答的实验研究 [J] . 中国普外基础与临床杂志, 2006; 13（6）：689 2 严茂林，程南生. 树突状细胞与消化系恶性肿瘤预后的研究进展 [J] . 中国普外基础与临床杂志, 2003; 10（5）：519.
2. Steinman RM, Pack M, Inaba K. Dendritic cells in the T-cell areas of lymphoid organs [J] . Immunol Rev， 1997; 156：25.
3. Lodge PA, Jones LA, Bader RA, et al . Dendritic cell-based immunotherapy of prostate cancer: immune monitoring of a phase Ⅱ clinical trial [J] . Cancer Res， 2000; 60(4)：829.
4. Bell D, Young JW, Banchereau J. Dendritic cells [J] . Adv Immunol， 1999; 72：255.
5. Nouri-Shirazi M, Banchereau J, Fay J, et al . Dendritic cell based tumor vaccines [J] . Immunol Lett， 2000; 74(1)：5.
6. Miller PW, Sharma S, Stolina M, et al . Intratumoral administration of adenoviral interleukin 7 gene-modified dendritic cells augments specific antitumor immunity and achieves tumor eradication [J] . Hum Gene Ther， 2000; 11(1)：53.
7. Celluzzi CM, Falo LD Jr. Physical interaction between dendritic cells and tumor cells results in an immunogen that induces protective and therapeutic tumor rejection [J] . J Immunol， 1998; 160(7)：3081.
8. Fong L, Brockstedt D, Benike C, et al . Dendritic cells injected via different routes induce immunity in cancer patients [J] . J Immunol， 2001; 166(6)：4254.
9. 张艳林，薛刚. 肿瘤细胞裂解物致敏的树突状细胞对肝癌HepG2细胞杀伤的体外研究 [J] . 华西医学， 2007； 22（1）：18.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Anti-Colon Cancer Immunity of Whole Cell Lysates Pulsed Dendritic Cell

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