Detection and Significance of β-Glucuronidase mRNA in Human Liver and Kidney Tissues_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Bo ¹ , ZHANG Hong ² , LI Wei ³ , ZHOU Wenping. ¹

Department of Hepatobiliary, Shenyang General Hospital in Shenyang Army, Shenyang 110015, China;

Corresponding author：

Keywords：

β-glucuronidase; Liver; Kidney; Gene

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Objective　 To establish a new method for detection of β-glucuronidase (β-G) mRNA in human liver and kidney tissues.
Methods 　β-G mRNA expression was detected by reverse transcription polymerase chain reaction(RT-PCR) in 10 cases of normal liver tissues, 10 cases of normal kidney tissues and 8 cases of hepatocellular carcinoma tissues.
Results 　The expand products of β-G mRNA were expressed in liver and kidney tissues with similar size of 422 bp. The expression contents of β-G mRNA in liver and kidney normal tissues were different (1.71±0.32 vs 1.83±0.22) but without statistical significance (P gt;0.05). However, the expression content of β-G mRNA in hepatocellular carcinoma tissues was 3.88±0.86， which was significantly higher (P＜0.01) than that in normal liver tissues.
Conclusion 　β-G mRNA determination is feasible by checking β-G gene alignment in gene bank and designing draw matter in the tissue of liver and kidney. It may be very significant to explore the change of β-G mRNA in various tissues in studying of molecular mechanism.

Citation： YANG Bo,ZHANG Hong,LI Wei,ZHOU Wenping.. Detection and Significance of β-Glucuronidase mRNA in Human Liver and Kidney Tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(6): 652-655. doi: Copy

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2.	Ho KJ. Biliary electrolytes and enzymes in patients with and without gallstones [J]. Dig Dis Sci, 1996; 41(12)∶2409.
3.	杨波，朱善德，赵永光，等. 肝细胞内源性β-葡萄糖醛酸酶与胆红素结石关系的免疫电镜研究 [J]. 中华肝胆外科杂志， 2000; 6(3）∶123.
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11.	韩文秀，孟翔凌，徐阿曼，等. 内源性β-葡萄糖醛酸酶与原发性肝内胆管结石关系的研究 [J]. 消化外科, 2006; 5（5）∶361.
12.	王宏林，郭仁宣，刘小芳，等. 乙肝病毒感染者胆汁及血清内内源性β-葡萄糖醛酸酶含量与胆红素结石的关系 [J]. 中华肝胆外科杂志, 2001; 7(6)∶346.
13.	Ho KJ. Pathogenesis of the formation of pigment gallstones. the role of human biliary β-glucuronidase [J]. Med Today, 1986; 155(8)∶695.
14.	朱善德，杨波，张弘，等. 肝癌患者癌组织中β-葡萄糖醛酸酶表达及临床意义 [J]. 肿瘤防治研究， 1999； 26（5）∶658.
15.	Bosslet K, Czech J, Lorenz P, et al. Molecular and functional characterisation of a fusion protein suited for tumour specific prodrug activation [J]. Br J Cancer, 1992; 65(2)∶234.
16.	Kappler M, Kotzsch M, Bartel F, et al. Elevated expression level of survivin protein in soft-tissue sarcomas is a strong independent predictor of survival [J]. Clin Cancer Res, 2003; 9(3)∶1098.
17.	杨波，张弘，朱善德，等. 肝癌患者血清及癌组织中β-葡萄糖醛酸酶同步测定及意义 [J]. 中华实验外科杂志， 1999；16（1）∶42.
18.	杨波，张弘，李为，等. 肝癌患者血清及癌组织中β-葡萄糖醛酸酶活性改变及临床意义 [J]. 世界华人消化杂志， 1999； 7（5）∶432.
19.	肖卫东，彭承宏，王梦龙, 等. RT-PCR法检测常规病理检查淋巴结阴性胃癌的淋巴结微转移 [J]. 中国普外基础与临床杂志, 2003; 10（5）∶487.
20.	杨波，张弘，李为，等. 不同分化程度肝癌组织中β-葡萄糖醛酸酶mRNA的表达及意义 [J]. 中华肿瘤防治研究杂志， 2007; 14（7）∶538.

1. 　Ho KJ, Hsu SC, Chen JS, et al. Human biliary beta-glucuronidase: correlation of its activity with deconjugation of bilirubin in the bile [J]. Eur J Clin Invest, 1986; 16(5)∶361.
2. 　Ho KJ. Biliary electrolytes and enzymes in patients with and without gallstones [J]. Dig Dis Sci, 1996; 41(12)∶2409.
3. 　杨波，朱善德，赵永光，等. 肝细胞内源性β-葡萄糖醛酸酶与胆红素结石关系的免疫电镜研究 [J]. 中华肝胆外科杂志， 2000; 6(3）∶123.
4. 　杨波，朱善德，杜晓矩，等. 人肝细胞超微结构β-葡萄糖醛酸酶分布的定位定量研究及意义 [J]. 中华实验外科杂志， 1996; 13（5）∶342.
5. 　Maki T. Pathogenesis of calcium bilirubinate gallstone: role of E. coli, beta-glucuronidase and coagulation by inorganic ions, polyelectrolytes and agitation [J]. Ann Surg, 1966; 164(1)∶90.
6. 　黄志强. 原发性肝胆管结石症 [A]. 见：吴阶平，裘法祖主编.黄家驷外科学 [M]. 第6版. 北京：人民卫生出版社， 1999∶1282~1285.
7. 　黄志强, 杨可贞，孟宪钧，等. 胆汁β-葡萄糖醛酸酶活性的意义 [J]. 中华外科杂志， 1982； 20（1）∶49.
8. 　杨波，朱善德，张弘，等. 胆红素结石与体液中内源性β-葡萄糖醛酸酶活性变化的关系 [J]. 中华实验外科杂志， 2000； 17（5）∶470.
9. 　杨波，吕东文，王洪林, 等. 不同胆结石患者胆汁和唾液中内源性β-葡萄糖醛酸酶活性比较 [J]. 中国普外基础与临床杂志, 2001; 8（2）∶113.
10. 　杨波，朱善德，赵永光, 等. 肝组织内源性β-葡萄糖醛酸酶活性与胆红素结石的关系 [J]. 中国普外基础与临床杂志, 1999; 6(5)∶278.
11. 　韩文秀，孟翔凌，徐阿曼，等. 内源性β-葡萄糖醛酸酶与原发性肝内胆管结石关系的研究 [J]. 消化外科, 2006; 5（5）∶361.
12. 　王宏林，郭仁宣，刘小芳，等. 乙肝病毒感染者胆汁及血清内内源性β-葡萄糖醛酸酶含量与胆红素结石的关系 [J]. 中华肝胆外科杂志, 2001; 7(6)∶346.
13. 　Ho KJ. Pathogenesis of the formation of pigment gallstones. the role of human biliary β-glucuronidase [J]. Med Today, 1986; 155(8)∶695.
14. 　朱善德，杨波，张弘，等. 肝癌患者癌组织中β-葡萄糖醛酸酶表达及临床意义 [J]. 肿瘤防治研究， 1999； 26（5）∶658.
15. 　Bosslet K, Czech J, Lorenz P, et al. Molecular and functional characterisation of a fusion protein suited for tumour specific prodrug activation [J]. Br J Cancer, 1992; 65(2)∶234.
16. 　Kappler M, Kotzsch M, Bartel F, et al. Elevated expression level of survivin protein in soft-tissue sarcomas is a strong independent predictor of survival [J]. Clin Cancer Res, 2003; 9(3)∶1098.
17. 　杨波，张弘，朱善德，等. 肝癌患者血清及癌组织中β-葡萄糖醛酸酶同步测定及意义 [J]. 中华实验外科杂志， 1999；16（1）∶42.
18. 　杨波，张弘，李为，等. 肝癌患者血清及癌组织中β-葡萄糖醛酸酶活性改变及临床意义 [J]. 世界华人消化杂志， 1999； 7（5）∶432.
19. 　肖卫东，彭承宏，王梦龙, 等. RT-PCR法检测常规病理检查淋巴结阴性胃癌的淋巴结微转移 [J]. 中国普外基础与临床杂志, 2003; 10（5）∶487.
20. 　杨波，张弘，李为，等. 不同分化程度肝癌组织中β-葡萄糖醛酸酶mRNA的表达及意义 [J]. 中华肿瘤防治研究杂志， 2007; 14（7）∶538.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Detection and Significance of β-Glucuronidase mRNA in Human Liver and Kidney Tissues

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