THE COMPARATIVE STUDY ON THE ESTABLISHMENT AND ITS CONDITION FOR THE CLONE OF RECIPIENT AGAINST DONOR ANTIGEN-SPECIFIC T LYMPHOCYTES_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHOU Jun , WANG Lei , LAN Ping , et al.

Department of Gastrointestinal Surgery, First Affiliated Hospital, Zhongshan Univeristy of Medical Sciences, Guangzhou 510080;

Corresponding author：

Keywords：

Donor antigen-specific T cell clone Limited-dilute method Raise cell

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【Abstract】Objective To explore the feasibility that the recipient against donor antigen-specific T lymphocytes clones are formed,and the suicide genes are induced into the clone. In the end it may induce the transplanted-organ tolerance. Methods The recipient rats were immunized by the donor rats-splenocytes, then the recipient’s T cell were isolated, purified and diluted with limited-dilute methods into single cell.The T cells were cultured by adding raise cells,ConA or IL2 under the different concentration and at last the T cell clone were formed. Results A stable recipient against donor antigen-specific T cell clone was established. The difference among the different immune groups was very significant(t gt;t0.05). The T cell clone was not formed without raise cells or only with raise cells. The rare clone could be formed by ConA stimulation but without IL-2. The clone-forming rate was associated with concentration of IL-2. Conclusion The mature T cell is able to proliferate to form clone when the condition is suitable. The recipient is immunized with donor rats spleen cellular antigen. The T cells clones selected in the end are donor antigen-specific.

Citation： ZHOU Jun,WANG Lei,LAN Ping,et al.. THE COMPARATIVE STUDY ON THE ESTABLISHMENT AND ITS CONDITION FOR THE CLONE OF RECIPIENT AGAINST DONOR ANTIGEN-SPECIFIC T LYMPHOCYTES. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(5): 292-294. doi: Copy

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1. 王国春，吴东海，Csennok E等. 韦格纳肉芽肿病T细胞表达及分泌细胞因子的研究: Tn1型免疫反应〔Ｊ〕. 中华风湿病学杂志，1998; 2(2)∶79.
2. 王重庆，李启靖，关迎辉等. 小鼠皮肤移植特异耐受性的获得〔Ｊ〕. 北京大学学报(自然科学版),1998; 34(4)∶481.
3. 沈关心，周汝麟主编. 现代免疫学实验技术〔M〕. 第1版. 武汉: 湖北科技出版社, 1998∶283~305.
4. 卓安山，陈爱娟，申繁星等.烫伤对大鼠T、B淋巴细胞功能影响及免疫调节剂对其作用研究〔Ｊ〕.中国实验临床免疫学杂志,1998； 10(2)∶29.
5. Legge KL，Min B，Pack C， et al. Differetial presentation of an altered peptide within fetal central and peripheral organs supports an avidity model for thymic T cell development and implies a peripheral readjustment for activation 〔Ｊ〕. J Immunol，1999; 162(10)∶5738.
6. BenBasson SZ，William EP，Ethan MS，et al. In vitro selection and extended culture of antigenspecific T lymphocytes 〔Ｊ〕. J Immunol，1975; 115（6）∶1723.
7. Gillis S，Baker P，Francis W，et al. Longterm culture of human antigenspecific cytotoxic Tcell lines 〔Ｊ〕. J Exp Med, 1978; 148（4）∶1093.
8. Wingren AG，Parra E，Varga M， et al. T cell activation pathway: B7，LFA3，and ICAM1 shape unique T cell profiles 〔Ｊ〕. Critical Rew Immunol，1995; 15（3-4）∶235.
9. Boussiotis Va，Gribben JG，Freeman GJ，et al. Blockade of the CD28 costimulatory pathway: a means to induce tolerance 〔Ｊ〕. Curr Opin Immunol，1994; 6（5）∶797.
10. 巴德年主编.当代免疫学技术与应用〔M〕.第1版.北京: 联合出版社,1998∶177~182.
11. 罗云萍，张定凤. T淋巴细胞克隆技术〔Ｊ〕.重庆医科大学学报,1992； 17(2)∶159.
12. 李子海，朱立平，谢少文等. T淋巴细胞克隆技术〔Ｊ〕.中国免疫学杂志,1987； 3(2)∶114.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE COMPARATIVE STUDY ON THE ESTABLISHMENT AND ITS CONDITION FOR THE CLONE OF RECIPIENT AGAINST DONOR ANTIGEN-SPECIFIC T LYMPHOCYTES

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