• Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China;
Glycation end products,advanced/diagnostic use; Diabetic retinopathy/physio-pathology; Transforming growth factors β ; Retina/drug effects; Pericytes
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Objective To investigate the effects of advanced glycation endproducts (AGEs) on proliferation of pericytes of bovine retinal capillary vessels and expression of transforming growth factor  beta;(TGF- beta;). Methods The proliferation of pericytes detected by methyl thiazolyl tetrazolium (MTT) colorimetric assay, cellular cycle of pericytes was analyzed by flow cytometry was used to analyze cell, and TGF- beta; protein expression of pericytes was observed by immunofluorescent staining. Results AGEs inhibited the proliferation of pericytes of bovine retinal capillary vessels, stopped the cellular cycle of pericytes in synthesis phase (S phase), increased the number of apoptotic cells obviously (P lt;0.01), and promoted the expression of TGF- beta; proteinof perycytes. Conclusions AGEs may promote the apoptosis of pericytes by inhibiting the proliferation of pericytes to lead the decrease of pericytes number, and may accelerate diabetic retinopathy by promoting the expression of TGF- beta; protein of pericytes. (Chin J Ocul Fundus Dis, 2006, 22: 20-23)

Citation： WANG Yuehui,NI Jinsong,CHEN Lanyue,et al. Effects of advanced glycation endproducts on proliferation of pericytes of bovine retinal capillary vessels and expression of transforming growth factor β. Chinese Journal of Ocular Fundus Diseases, 2006, 22(1): 20-23. doi: Copy