Objective To investigate the protective effect of aminoguanidine(AG),silymarin (Sil) and anisodamine (Ani) on retinal capillary pericytes cultured in glycosylation products. Methods MTT cololrimetric assay, [3H] thymidine incorporating and fluorescent indicat or fura-2 acetoxy-methyl ester (Fura-2AM) were used to study the influence of AG,Sil and Ani on the growth,DNA synthesis,and cytosolic free calcium ([Ca2 ]i)changes of pericytes cultured in the medium contained early glycation products (EGs) or advanced glycation end products (AGEs). Results Cultured in the medium contained EGs,the A value by MTT assayed and amount of [3H] thymidine incorporating in AG group and Sil group were obviously elevated than those of control group(P lt;0.01);but the [Ca2 ]iconcentration in both groups were decreased significantly comparing with control group(P lt;0.01 and 0.05).Under the condition of AEGs,only AG group was distinctly increased on the A value and amount of [3H] thymidine incorporatin g (P lt;0.01),and [Ca2 ]i concentration was markedly decreased (P lt;0.05) comparing with control group. Conclusion AG has the portective effect on pericytes against the proliferative inhibition and excessive elevation of [Ca2 ]i concentration in cytosol which are induced both by EGs or AGEs.Silymarin has the effect for those only by Egs-induced.Ani has no protective effect no pericytes nei ther cultured in medium with EGs nor with AGEs. (Chin J Ocul Fundus Dis, 2001,17:192-194)

Citation： XI Xinghua,JIANG Deyong,TANG Luosheng.. The protection of aminoguanidine,silymarin and anisodamine on growth and cytosolic free calcium changes of retinal capillary pericytes cultured in glycation products. Chinese Journal of Ocular Fundus Diseases, 2001, 17(3): 192-194. doi: Copy