The effect of posterior sub-Tenon capsule injection of triamcinolone acetonide to inflammatory cytokines in rat retina after pan-retinal photocoagulation_Chinese Journal of Ocular Fundus Diseases

Authors：

LuDan ,  WangKang , LiNa , 于书静 , 王艳玲 , 李明铭 , 郭建友

Department of Ophthalmology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;

Corresponding author：

WangKang, Email: bnbn2000@163.com

Keywords：

Diabetic retinopathy/complications; Macular edema/drug therapy; Laser coagulation; Triamcinolone acetonide/therapeutic use; Chemokines

DOI：

10.3760/cma.j.issn.1005-1015.2014.02.013

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Objective To observe the expression of inflammatory cytokines in diabetic rats received posterior sub-Tenon capsule injection of triamcinolone acetonide (TA) and pan-retinal photocoagulation. Methods A total of 48 Brown Norway rats received intraperitoneal injection of streptozotocin to establish the diabetic model. Diabetic rats were randomly divided into experimental group (20 rats), control group (20 rats) and blank group (8 rats). 50 μl TA or saline was injected into the posterior sub-Tenon capsule immediately after the photocoagulation in the experimental group and the control group, respectively. The blank group received no treatment. The mRNA and protein expression level of retinal vascular endothelial growth factor (VEGF), interleukin-6 (IL-6) and tumor necrosis fator-α (TNF-α) were measured by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) at 1, 3, 7 days after laser photocoagulation. Results The mRNA and protein expression of VEGF, IL-6, TNF-α of the experimental group and control group were significantly higher than the blank group, the difference was statistically significant (P < 0.05). The mRNA and protein expression of VEGF, IL-6 and TNF-α of the experimental group were significantly lower than that of the control group. On day 1 after laser photocoagulation, the mRNA expression of VEGF was not statistically significant in the experimental group and control group (P > 0.05), the mRNA and protein expression of VEGF, IL-6, TNF-α of the two groups were statistically significant in the remaining observing time (P < 0.05). Conclusion Posterior sub-Tenon capsule injection of TA can effectively reduce retinal photocoagulation induced VEGF, IL-6, TNF-α expression.

Citation： LuDan, WangKang, LiNa. The effect of posterior sub-Tenon capsule injection of triamcinolone acetonide to inflammatory cytokines in rat retina after pan-retinal photocoagulation. Chinese Journal of Ocular Fundus Diseases, 2014, 30(2): 171-174. doi: 10.3760/cma.j.issn.1005-1015.2014.02.013 Copy

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8.	Hoekzema R, Verhagen C, van Haren M, et al. Endotoxin-induced uveitis in the rat:the significance of intraocular interleukin-6[J]. Invest Ophthalmol Vis Sci, 1992, 33:532-539.
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10.	Sfikakis PP, Markomichelakis N, Tneodossiadis GP, et al. Regression of sight-threatening macular edema in type-2 diabetes following treatment with the anti-tumor Necrosis factor monoelonal antibody infliximab[J].Diabetes Care, 2005, 28:445-447.
11.	Demircan N, Safran BG, Soylu M, et al. Determination of vitreous sinter leukin-1(IL-1)and tumor necrosis factor(TNF)levels in proliferative diabetic retinopathy[J]. Eye, 2006, 20:1366-1369.

1. Tamura H, Miyamoto K, Kiryu J, et al. Intravitreal injection of corticosteroid attenuates leukostasis and vascular leakage in experimental diabetic retina[J]. Invest Ophthalmol Vis Sci, 2005, 46:1440-1444.
2. Basel T, Farid H. Posterior sub-Tenon injection of triamcinolone acetonide for cystiod diabetic macular edema[J]. J R Med Serv, 2010, 17:60-64.
3. McDonald HR, Schatz H. Macular edema following panretinal photocoagulation[J]. Retina, 1985, 5:5-10.
4. Shimura M, Yasuda K, Nakazawa T, et al. Quantifying alterations of macular thickness before and after panretinal photocoagulation in patients with severe diabetic retinopathy and good vision[J]. Ophthalmology, 2003, 110:2386-2394.
5. Nonaka A, Kiryu J, Tsujikawa A, et al. Inflammatory response after scatter laser photocoagulation in nonphotocoagulated retina[J]. Invest Ophthalmol Vis Sci, 2002, 43:1204-1209.
6. Massin P, Audren F, Haouchine B, et al. Intravitreal triamcinolone acetonide for diabetic diffuse macular edema:preliminary results of a prospective controlled trial[J]. Ophthalmology, 2004, 111:218-224.
7. Shimura M, Nakazawa T, Yasuda K, et al. Pre-treatment of posterior sub-Tenon injection of triamcinolone acetonide has beneficial effects for grid pattern photocoagulation against diffuse diabetic macular edema[J].Br J Ophthalmol, 2007, 91:449-454.
8. Hoekzema R, Verhagen C, van Haren M, et al. Endotoxin-induced uveitis in the rat:the significance of intraocular interleukin-6[J]. Invest Ophthalmol Vis Sci, 1992, 33:532-539.
9. Koskela UE, Kuusisto SM, Nissinen AE, et al. High vitreous concentration of IL-6 and IL-8, but not of adhesion molecules in relation to plasma concentrations in proliferative diabetic retinopathy[J]. Ophthalmic Res, 2013, 49:108-114.
10. Sfikakis PP, Markomichelakis N, Tneodossiadis GP, et al. Regression of sight-threatening macular edema in type-2 diabetes following treatment with the anti-tumor Necrosis factor monoelonal antibody infliximab[J].Diabetes Care, 2005, 28:445-447.
11. Demircan N, Safran BG, Soylu M, et al. Determination of vitreous sinter leukin-1(IL-1)and tumor necrosis factor(TNF)levels in proliferative diabetic retinopathy[J]. Eye, 2006, 20:1366-1369.

Chinese Journal of Ocular Fundus Diseases

The effect of posterior sub-Tenon capsule injection of triamcinolone acetonide to inflammatory cytokines in rat retina after pan-retinal photocoagulation

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