Application of Micron Ⅳ retinal imaging system in three different types of mouse models_Chinese Journal of Ocular Fundus Diseases

Authors：

LiuShiliang ,  ShenYin , ChenYuanyuan , 胡单萍 , 田凯琳 , 陈长征 , 邢怡桥

Eye Center, Renmin Hospital of Wuhan University, Wuhan 430060, China;

Corresponding author：

ShenYin, Email: yinshen@whu.edu.cn

Keywords：

Diagnostic imaging; Fluorescein angiography/utilization; Tomography, optical coherence/utilization; Electroretinography/utilization; Models, animal

DOI：

10.3760/cma.j.issn.1005-1015.2014.03.016

Video：

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Objective To evaluate Micron Ⅳ retinal imaging system in three mouse models of retinal diseases. Methods Mouse models of oxygen induced retinopathy (OIR) model (OIR group), N-methyl-N nitrosourea (MNU) model (MNU group) and N-methyl-D-aspartate (NMDA) model (NMDA group) were induced in 24 healthy male C57BL/6J mice. Fundus photograph, fundus fluorescein angiography (FFA) and optical coherence tomography (OCT) and electroretinogram (ERG) were used to evaluate these mice. All the imaging examinations were performed by Micron Ⅳ retinal imaging system. Results OIR mice showed tortuous and dilated retinal vessels in fundus photograph, neovascularization plexus and vascular leakage in FFA, and epiretinal fibrovascular tissue and tortuous expansion vascular vessels in OCT. MNU mice showed wax yellow optic disk without retinal pigmentary changes, slight thinning of retinal blood vessels in FFA, and normal structure and thickness in OCT. The a-wave amplitudes of the maximum mixed response decreased significantly, and were (15.38±4.36) μV and (13.78±5.52) μV at 2 or 3 days of modeling, respectively. NMDA mice showed a pale retina with vasospasm. ERG revealed that there was no obvious change in latency of a- and b-wave, but significantly decreased amplitude of b-wave at 12 hours and 24 hours after modeling with (72.28±7.18) μV and (65.35±9.18) μV, respectively. Conclusion Micron Ⅳ retinal imaging system is a real-time, non-invasive tool to study the retinal structure and function in animal models of retinal diseases.

Citation： LiuShiliang, ShenYin, ChenYuanyuan. Application of Micron Ⅳ retinal imaging system in three different types of mouse models. Chinese Journal of Ocular Fundus Diseases, 2014, 30(3): 294-298. doi: 10.3760/cma.j.issn.1005-1015.2014.03.016 Copy

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7.	Chen YY,Liu SL,Hu DP, et al. N-methyl-N-nitrosourea-induced retinal degeneration in mice[J]. Exp Eye Res,2014, 121: 102-113.
8.	Jeong E, Paik SS, Jung SW, et al. Morphological and functional evaluation of an animal model for the retinal degeneration induced by N-methyl-N-nitrosourea[J]. Anat Cell Biol, 2011, 44: 314-323.
9.	Seki M, Lipton SA. Targeting excitotoxic/free radical signaling pathways for therapeutic intervention in glaucoma[J]. Prog Brain Res, 2008, 173: 495-510.
10.	Shen Y, Liu XL, Yang XL. N-methyl-D-aspartate receptors in the retina[J]. Mol Neurobiol, 2006, 34: 163-179.
11.	吴建国,李颖,周小煦,等. Micron Ⅲ视网膜影像系统大鼠荧光素眼底血管造影[J]. 中华眼底病杂志,2013,29:523-525.
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1. Smith LE, Wesolowski E, McLellan A, et al. Oxygen-induced retinopathy in the mouse[J]. Invest Ophthalmol Vis Sci, 1994, 35: 101-111.
2. Yuge K, Nambu H, Senzaki H, et al. N-methyl-N-nitrosourea-induced photoreceptor apoptosis in the mouse retina[J]. In Vivo, 1996, 10: 483-488.
3. Seitz R, Tamm ER. N-methyl-D-aspartate (NMDA)-mediated excitotoxic damage: a mouse model of acute retinal ganglion cell damage[J]. Methods Mol Biol, 2013, 935: 99-109.
4. Grossniklaus HE, Kang SJ, Berglin L. Animal models of choroidal and retinal neovascularization[J]. Prog Retin Eye Res, 2010, 29: 500-519.
5. Browning J, Wylie CK, Gole G. Quantification of oxygen-induced retinopathy in the mouse[J]. Invest Ophthalmol Vis Sci, 1997, 38: 1168-1174.
6. Yoshizawa K, Tsubura A. Characteristics of N-methyl-N-nitrosourea-induced retinal degeneration in animals and application for the therapy of human retinitis pigmentosa[J]. Nihon Ganka Gakkai Zasshi, 2005, 109: 327-337.
7. Chen YY,Liu SL,Hu DP, et al. N-methyl-N-nitrosourea-induced retinal degeneration in mice[J]. Exp Eye Res,2014, 121: 102-113.
8. Jeong E, Paik SS, Jung SW, et al. Morphological and functional evaluation of an animal model for the retinal degeneration induced by N-methyl-N-nitrosourea[J]. Anat Cell Biol, 2011, 44: 314-323.
9. Seki M, Lipton SA. Targeting excitotoxic/free radical signaling pathways for therapeutic intervention in glaucoma[J]. Prog Brain Res, 2008, 173: 495-510.
10. Shen Y, Liu XL, Yang XL. N-methyl-D-aspartate receptors in the retina[J]. Mol Neurobiol, 2006, 34: 163-179.
11. 吴建国,李颖,周小煦,等. Micron Ⅲ视网膜影像系统大鼠荧光素眼底血管造影[J]. 中华眼底病杂志,2013,29:523-525.
12. Goode GB.Retinal imaging advances research,disease diagnosis[J/OL].Bio Optics World,2010,3:[2012-06-10].http://www.bioopticsworld.com/articles/print/volume-3/issue-3/features/opthalmology-medieal-imaging.html.

Chinese Journal of Ocular Fundus Diseases

Application of Micron Ⅳ retinal imaging system in three different types of mouse models

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