The characteristics of the full-field flash electroretinogram in rats with oxygen induced retinopathy_Chinese Journal of Ocular Fundus Diseases

Authors：

Xie Li , Luo Yi ,  Wang Ping , Ma Lan , Li Na , Xie Linhui

Department of Ophthalmology, Hunan Children's Hospital, The Pediatric College of University of South China, Changsha 410007, China (Xie Li, now working at Department of Ophthalmology, The Maternal and Child Healthcare Hospital of Xiangtan City; Li Na, now working at Changsha Honglang Eye Hospital);

Corresponding author：

Wang Ping, Email: wangping22@qq.com

Keywords：

Retinal diseases/physiopathology; Electroretinography; Animal experimentation

DOI：

10.3760/cma.j.issn.1005-1015.2017.02.017

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Objective To observe the characteristics of the full-field flash electroretinogram (F-ERG) in rats with oxygen induced retinopathy (OIR). Methods Twenty-four neonatal Sprague Dawley rats were divided into OIR group and control group. In OIR group, 12 rats were exposed to (75±2)% oxygen for 7 days and then to room air for 7 days; in control group, 12 rats were raised in room air for 14 days. At postnatal day 21, F-ERG tests were performed to examine the rod response , the maximum mixing reaction and the cone reaction. Results Compared with the control group, the b-wave amplitudes decreased (t=3.650) and the implicit times increased (t=2.410) in rod response in OIR group, the differences were statistically significant (P＜0.05); the a- and b-wave amplitudes decreased (t=3.333, 2.562) and the implicit times increased (t=2.725, 2.482) in the maximum mixing reaction in OIR group, the differences were statistically significant (P＜0.05). There was no difference between OIR and control group on a- and b-wave amplitudes (t=0.650, 0.204) and implicit times (t=0.422, 0.076) in cone response (P＞0.05). 0.001 cd.s/m² light intensity stimulation on rats F-ERG wave almost no response. 0.010 cd.s/m² light intensity stimulation on rats can be recorded to the rod response waveform, with the increase of light intensity, the amplitude of b-wave increases, the a-wave extraction. Conclusions F-ERG of OIR rat showed that the amplitude and sensitivity of the rod response and maximal rod-cone response was decreased. The intensity of light had effect on the OIR rod cells, and the amplitude of b- wave increased with the increase of light intensity, the a-wave extraction.

Citation： Xie Li, Luo Yi, Wang Ping, Ma Lan, Li Na, Xie Linhui. The characteristics of the full-field flash electroretinogram in rats with oxygen induced retinopathy. Chinese Journal of Ocular Fundus Diseases, 2017, 33(2): 186-189. doi: 10.3760/cma.j.issn.1005-1015.2017.02.017 Copy

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9.	Madan A, Penn JS. Animal models of oxygen-induced retinopathy[J]. Front Biosci, 2003, 8: 1030-1043.
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12.	Fulton AB, Hansen RM, Peter RA, et al. The rod photoreceptors in retinopathy of prematurity [J]. Arch Ophthalmol, 2001, 119(4): 499-505.
13.	Grün G. The development of the vertebrate retina: a comparative survey[J]. Adv Anat Embryol Cell Biol, 1982, 78: 1-85.
14.	Fulton AB, Dodge J, Hansen RM, et al. The rhodopsin content of human eyes[J]. Invest Ophthalmol Vis Sci, 1999, 40(8): 1878-1883.

1. 王平, 陶利娟, 唐景, 等. 阈值和阈值前期早产儿视网膜病变儿童视网膜电图观察[J]. 中国斜视与小儿眼科杂志, 2014, 22(1): 1-4.Wang P, Tao LJ, Tang J, et al. Full-field flash electroretinogram following up for children with history of pre-threshold or threshold ROP[J]. Chinese Journal of Strabismus & Pediatric Ophthalmology, 2014, 22(1): 1-4.
2. 王平, 田密, 张金梅, 等. 阈值和阈值前期早产儿视网膜病变的远期视网膜电图特征[J]. 中华眼底病杂志, 2014, 30(1): 21-23. DOI: 10.3760/cma.j.issn.1005-1015.2014.01.006.Wang P, Tian M, Zhang JM, et al. Full-field flash electroretinogram following up for children with history of pre-threshold or threshold retinopathy of prematurity[J]. Chin J Ocul Fundus Dis, 2014, 30(1): 21-23. DOI: 10.3760/cma.j.issn.1005-1015.2014.01.006.
3. Fulton AB, Hansen RM, Moskowitz A. The electroretinogram in retinopathy of prematurity[J]. Invest Ophthalmol Vis Sci, 2008, 49 (2) : 814-819. DOI: 10.1167/iovs.07-1226.
4. Fulton AB, Hansen RM, Moskowitz A. Development of rod function in term born and for preterm subjects[J]. Optom Vis Sci, 2009, 86 (6) : 653-658. DOI: 10.1097/OPX.0b013e3181a6a237.
5. Yang CS, Wang AG, Shih YF, et al. Long-term biometric optic components of diode laser-treated threshold retinopathy of prematurity at 9 years of age[J]. Acta Ophthalmol, 2013, 91(4): 276-282. DOI: 10.1111/aos.12053.
6. Chen Y, Lien RI, Tsai S, et al. Natural history of retinopathy of prematurity: two-year outcomes of a prospective study[J]. Retina, 2015, 35(1): 141-148. DOI: 10.1097/IAE.0000000000000270.
7. Fruttiger M. Development of the mouse retinal vasculature: angiogenesis versus vasculogenesis[J]. Invest Ophthalmol Vis Sci, 2002, 43: 522-527.
8. Marmor MF, Holder GE, Seeliger MW, et al. Standard for clinical electroretinography (2004 update) [J]. Doc Ophthalmol, 2004, 108(2): 107-114.
9. Madan A, Penn JS. Animal models of oxygen-induced retinopathy[J]. Front Biosci, 2003, 8: 1030-1043.
10. Gossman, Mitchell Vincent MD. Systematic evaluation of the mouse eye: anatomy, pathology, and biomethods[J]. J Neuro-Ophthalmol, 2004, 24(1): 84-85.
11. Liu K, Akula JD, Falk C, et al. The retinal vasculature and function of the neural retina in a rat model of retinopathy of prematurity[J]. Invest Ophthalmol Vis Sci, 2006, 47(6): 2639-2647.
12. Fulton AB, Hansen RM, Peter RA, et al. The rod photoreceptors in retinopathy of prematurity [J]. Arch Ophthalmol, 2001, 119(4): 499-505.
13. Grün G. The development of the vertebrate retina: a comparative survey[J]. Adv Anat Embryol Cell Biol, 1982, 78: 1-85.
14. Fulton AB, Dodge J, Hansen RM, et al. The rhodopsin content of human eyes[J]. Invest Ophthalmol Vis Sci, 1999, 40(8): 1878-1883.

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