Study on <i>In Vitro</i> Biomineralization of Enamel-binding Peptide_Journal of Biomedical Engineering

Authors：

WEIWei ¹ , PENGZhou ² , DENGJie ¹ , ZHANGWei ¹ ,  MAOJing ¹

1. Department of Orthodontics, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China;
2. College of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China;

Corresponding author：

MAOJing, Email: vivi200207@qq.com

Keywords：

enamel-binding peptide; biomineralization; single-diffusion gel system

DOI：

10.7507/1001-5515.20140026

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Abstract Full text Figures/Tables Video References Cited by

We present the binding ability of a new peptide (-CMPQVMPMC-) with dental enamel after being evaluated in the present study. Under a standard procedure, the recovery of M13 filamentous phage was greatly enhanced by displaying the peptide in phage coat protein pⅢ. Then the cyclic peptide was synthesized using a solid method. The effect of the cyclic peptide in vitro biomineralization was tested in a single-diffusion microtiter plate gel system. Absorbance at 405 nm of each sample was recorded for 24 h at every 6 h intervals. The relatively increased values of each sample were expressed as percentages relative to the blank group (100%). The cyclic peptide resulted in a concentration-dependent delayed nucleation. In addition, the overall values of peptide groups at the end of 24 h were lower than those in the control group but much higher than those in the BSA control group.

Citation： WEIWei, PENGZhou, DENGJie, ZHANGWei, MAOJing. Study on In Vitro Biomineralization of Enamel-binding Peptide. Journal of Biomedical Engineering, 2014, 31(1): 132-135. doi: 10.7507/1001-5515.20140026 Copy

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1. ZHOU B, LIU Y, WEI W,et al. GEPIs-HA hybrid:a novel biomaterial for tooth repair[J]. Med Hypotheses,2008,71(4):591-593.
2. SEKER U O, DEMIR H V. Material binding peptides for nanotechnology[J]. Molecules, 2011,16(2):1426-1451.
3. ROY M D, STANLEY S K, AMIS E J, et al. Identification of a highly specific hydroxyapatite-binding peptide using phage display[J]. Advanced Materials, 2008, 20(10):1830-1836.
4. LI C M, BOTSARIS G D, KAPLAN D L. Selective in vitro effect of peptides on calcium carbonate crystallization[J]. Crystal Growth Design, 2002, 2(5):387-393.
5. 茆灿泉,刘畅,马春燕,等. HA结合肽的筛选与序列分析[J].中国生物工程杂志,2005, 25 (10):25-28.
6. TIAN K, PENG M, REN X, et al. Regeneration of tooth-like hydroxyapatite depended on amelogenin functional section monolayer:a new approach for tooth repair[J]. Med Hypotheses,2012,79(2):143-146.
7. FUJISAWA R, WADA Y, NODASAKA Y, et al. Acidic amino acid-rich sequences as binding sites of osteonectin to hydroxyapatite crystals[J]. Biochim Biophys Acta,1996,1292(1):53-60.
8. WASSELL D T, HALL R C, EMBERY G. Adsorption of bovine serum albumin onto hydroxyapatite[J]. Biomoterials, 1995, 16(9):697-702.
9. 沈玉华, 杨展澜, 吴瑾光. BSA-羟基磷灰石可溶性复合物的FTIR光谱[J]. 物理化学学报, 1999, 15(12):1061-1069.
10. SILVERMAN L, BOSKEY A L. Diffusion systems for evaluation of biomineralization[J].Calcif Tissue Int, 2004, 75(6):494-501.
11. BOSKEY A, SPEVAK L, TAN M, et al. Dentin sialoprotein (DSP) has limite deffects on in vitro apatite formation and growth[J]. Calcif Tissue Int, 2000, 67(6):472-478.

Journal of Biomedical Engineering

Study on In Vitro Biomineralization of Enamel-binding Peptide

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