Ectopic Osteogenesis <i>In Vivo</i> Using Bone Morphogenetic Protein-2 Derived Peptide Loaded Biodegradable Hydrogel_Journal of Biomedical Engineering

Authors：

 ZHAOJingjing , FANGZhenhua , HUANGRuokun , XIAOKai , LIJing , XIEMing , KANWusheng

Deparment of Orthopedics Surgery, Puai Hospital, Huazhong University of Science and Technology, Wuhan 430034, China;

Corresponding author：

ZHAOJingjing, Email: zhaojingjing017@163.com

Keywords：

bone tissue engineering; bone morphogenetic protein-2; hydrogel; osteoinduction

DOI：

10.7507/1001-5515.20140152

Video：

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Abstract Full text Figures/Tables Video References Cited by

We investigated the development of an injectable, biodegradable hydrogel composite of poly(trimethylene carbonate)-F127-poly(trimethylene carbonate)(PTMC₁₁-F127-PTMC₁₁)loaded with bone morphogenetic protein-2 (BMP-2) derived peptide P24 for ectopic bone formation in vivo and evaluated its release kinetics in vitro. Then we evaluated P24 peptide release kinetics from different concentration of PTMC₁₁-F127-PTMC₁₁ hydrogel in vitro using bicinchoninic acid (BCA)assay. P24/PTMC₁₁-F127-PTMC₁₁ hydrogel was implanted into each rat's erector muscle of spine and ectopic bone formation of the implanted gel in vivo was detected by hematoxylin and eosin stain (HE). PTMC₁₁-F127-PTMC₁₁ hydrogel with concentration more than 20 percent showed sustained slow release for one month after the initial burst release. Bone trabeculae surround the P24/PTMC₁₁-F127-PTMC₁₁ hydrogel was shown at the end of six weeks by hematoxylin and eosin stain. These results indicated that encapsulated bone morphogenetic protein (BMP-2) derived peptide P24 remained viable in vivo, thus suggesting the potential of PTMC₁₁-F127-PTMC₁₁ composite hydrogels as part of a novel strategy for localized delivery of bioactive molecules.

Citation： ZHAOJingjing, FANGZhenhua, HUANGRuokun, XIAOKai, LIJing, XIEMing, KANWusheng. Ectopic Osteogenesis In Vivo Using Bone Morphogenetic Protein-2 Derived Peptide Loaded Biodegradable Hydrogel. Journal of Biomedical Engineering, 2014, 31(4): 811-815. doi: 10.7507/1001-5515.20140152 Copy

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1. SAITO A, SUZUKI Y, OGATA S , et al. Activation of osteo-progenitor cells by a novel synthetic peptide derived from the bone morphogenetic protein-2 knuckle epitope[J]. Biochim Biophys Acta, 2003, 1651(1-2): 60-67.
2. 段智霞,郑启新,郭晓东,等.BMP2活性多肽/PLGA复合物植入异位成骨的实验研究[J].国际生物医学工程杂志,2007,30(3):129-132.
3. SAITO A, SUZUKI Y, KITAMURA M, et al. Repair of 20-mm long rabbit radial bone defects using BMP-derived peptide combined with an alpha-tricalcium phosphate scaffold[J]. J Biomed Mater Res A, 2006, 77(4): 700-706.
4. 于玮洁,王新刚,胡信雷,等.聚乳酸-羟基乙酸(PLGA)网架生物学性能的初步评价[J].生物医学工程学杂志,2011,1(1):163-169.
5. 罗丙红,全大萍,廖凯荣,等.PLGA-(PEG-ASP)n共聚物的合成与性能研究[J].功能高分子学报,2005,18(2):295-298.
6. ZHANG Z, FOKS M A, GRIJPMA D W, et al. PTMC and MPEG-PTMC microparticles for hydrophilic drug delivery[J]. J Control Release, 2005, 101(1-3): 392-394.
7. LI J F, LIN Z Y, ZHENG Q X, et al. Bone formation in ectopic and osteogenic tissue induced by a novel BMP-2-related peptide combined with rat tail collagen[J]. Biotechnol Bioprocess Eng, 2010, 15(5): 725-732.
8. ALTUNBAS A, LEE S J, RAJASEKARAN S A, et al. Encapsulation of curcumin in self-assembling peptide hydrogels as injectable drug delivery vehicles[J]. Biomaterials, 2011, 32(25): 5906-5914.
9. WU B, ZHENG Q X, GUO X D, et al. Preparation and ectopic osteogenesis in vivo of scaffold based on mineralized recombinant human-like collagen loaded with synthetic BMP-2-derived peptide[J]. Biomed Mater, 2008, 3(4): 44111.
10. GELAIN F, UNSWORTH L D, ZHANG S. Slow and sustained release of active cytokines from self-assembling peptide scaffolds[J]. J Control Release, 2010, 145(3): 231-239.
11. KOPESKY P W, VANDERPLOEG E J, KISIDAY J D, et al. Controlled delivery of transforming growth factor β1 by self-assembling peptide hydrogels induces chondrogenesis of bone marrow stromal cells and modulates Smad2/3 signaling[J]. Tissue Eng Part A, 2011, 17(1-2): 83-92.

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