Cytotoxicity of a New Type Silicone Rubber for Maxillofacial Prosthesis: an <i>In vitro</i> Evaluation_Journal of Biomedical Engineering

Authors：

TIANAi , CHENYue , LIAOJian , SUNXu , TENGMinhua ,  LIANGXing

West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;

Corresponding author：

LIANGXing, Email: xingliangdent@vip.163.com

Keywords：

silicone rubber; prosthesis; cytotoxicity; cell counting kit-8

DOI：

10.7507/1001-5515.20140196

Video：

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This study evaluated the cytotoxicity of a new type silicone rubber for maxillofacial prosthesis, which was developed by the present authors. According to the GB/T16886.5-2003, the samples were prepared and tested with cell counting kit-8 (CCK-8) assay, the relative growth rate (RGR) was calculated, and morphology of L929 cells were observed by scanning electron microscope and phase contrast microscope. The results showed that RGR of L929 cells were 91.65% (24 h), 87.03% (48 h), 87.30% (72 h), respectively, and the level of cytotoxicity was grade 1. The L929 cells showed typical fusiform shape and their morphology did not changed significantly after 24 h, 48 h and 72 h. These data indicated that the newly-developed silicone rubber material, as a maxillofacial prosthesis material, should be a safe biomaterial.

Citation： TIANAi, CHENYue, LIAOJian, SUNXu, TENGMinhua, LIANGXing. Cytotoxicity of a New Type Silicone Rubber for Maxillofacial Prosthesis: an In vitro Evaluation. Journal of Biomedical Engineering, 2014, 31(5): 1046-1049,1056. doi: 10.7507/1001-5515.20140196 Copy

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9.	NUNO G, HORACIO Z, LUIS A, et al. Microsurgical reconstruction of maxillectomy defects: experience of 24 cases[J]. European Journal of Plastic Surgery, 2013, 36(10): 619-626.
10.	KIM H, KIM D W, CHANG H, et al. Maxillectomy reconstruction with various flaps: preliminary results and suggested algorithm[J]. Otolaryngology-Head and Neck Surgery, 2013, 149(2): 145.
11.	SANTAMARIA E, CORDEIRO P G. Reconstruction of maxillectomy and midfacial defects with free tissue transfer[J]. J Surg Oncol, 2006, 94(6): 522-531.
12.	CHANG T L, GARRETT N, ROUMANAS E, et al. Treatment satisfaction with facial prostheses[J]. J Prosthet Dent, 2005, 94(3): 275-280.
13.	DOS SANTOS D M, GOIATO M C, PESQUEIRA A A, et al. Prosthesis auricular with osseointegrated implants and quality of life[J]. J Craniofac Surg, 2010, 21(1): 94-96.
14.	陈启林,马建光,崔有根.中空阻塞器义齿修复上颌骨缺损的临床效果[J].北京口腔医学,2001,9(1):29-30.
15.	钟志海,索万奎,郑美华,等.Mollosil软衬式赝复体修复单侧上颌骨缺损的初步评价[J].中山大学学报:医学科学版,2004,25(z1):214-216.
16.	陈良建,黄冬梅.充气式硅橡胶赝复体修复单侧上颌骨缺损的研究[J].口腔颌面修复学杂志,2009,10(3):157-161.
17.	杜兵,陈巨峰,符志锋.计算机辅助设计与制作在磁性固位分体式上颌骨赝复体修复中的应用[J].中华口腔医学研究杂志:电子版,2011,5(2):178-183.
18.	MOORE D J, GLASER Z R, TOGACOO M J, et al. Evaluation of polymeric materials for facial prosthetics[J]. J Prosthet Dent, 1977, 38(3): 319-326.
19.	赵信义.颌面赝复材料研究进展[J].国际生物医学工程杂志,2006,29(3):178-183.
20.	侯春梅,李新颖,叶伟亮,等.MTT法和CCK-8法检测悬浮细胞增殖的比较[J].军事医学科学院院刊,2009,33(4):400-402.
21.	辛培成,杨圣,芦健民,等.CCK-8法检测兔筋膜成纤维细胞增殖活性之最佳测试条件研究[J].中外医学研究,2011,9(12):6-8.

1. 马轩祥.口腔修复学[M].第7版.沈阳:辽宁科学技术出版社,1999:424.
2. ROGERS S N, LOWE D, MCNALLY D, et al. Health-related quality of Life after maxillectomy: a comparison between prosthetic obturation and free flap[J]. J Oral Maxillofac Surg, 2003, 61(2): 174-181.
3. GOIATO M C, PESQUEIRA A A, RAMOS DA SILVA C, et al. Patient satisfaction with maxillofacial prosthesis. Literature review[J]. J Plast Reconstr Aesthet Surg, 2009, 62(2): 175-180.
4. 许哲武,李彦.颌面赝复体硅橡胶改性的研究进展[J].国际口腔医学杂志,2010,37(1):98-100.
5. 李蕾.颌面赝复的研究进展[J].国际口腔医学杂志,2011,38(6):704-707.
6. KIAT-AMNUAY S,GETTLEMAN L,GOLDSMITH L J. Effect of multi-adhesive layering on retention of extraoral maxillofacial silicone prostheses in vivo [J]. J Prosthet Dent,2004,92(3):294-298.
7. BELLAMY K, LIMBERT G, WATERS M G, et al. An elastomeric material for facial prostheses: synthesis, experimental and numerical testing aspects[J]. Biomaterials, 2003, 24(27): 5061-5066.
8. 国家质量监督检验检疫总局. GB/T16886.1-12医疗器械生物学评价[S].北京:中国标准出版社.
9. NUNO G, HORACIO Z, LUIS A, et al. Microsurgical reconstruction of maxillectomy defects: experience of 24 cases[J]. European Journal of Plastic Surgery, 2013, 36(10): 619-626.
10. KIM H, KIM D W, CHANG H, et al. Maxillectomy reconstruction with various flaps: preliminary results and suggested algorithm[J]. Otolaryngology-Head and Neck Surgery, 2013, 149(2): 145.
11. SANTAMARIA E, CORDEIRO P G. Reconstruction of maxillectomy and midfacial defects with free tissue transfer[J]. J Surg Oncol, 2006, 94(6): 522-531.
12. CHANG T L, GARRETT N, ROUMANAS E, et al. Treatment satisfaction with facial prostheses[J]. J Prosthet Dent, 2005, 94(3): 275-280.
13. DOS SANTOS D M, GOIATO M C, PESQUEIRA A A, et al. Prosthesis auricular with osseointegrated implants and quality of life[J]. J Craniofac Surg, 2010, 21(1): 94-96.
14. 陈启林,马建光,崔有根.中空阻塞器义齿修复上颌骨缺损的临床效果[J].北京口腔医学,2001,9(1):29-30.
15. 钟志海,索万奎,郑美华,等.Mollosil软衬式赝复体修复单侧上颌骨缺损的初步评价[J].中山大学学报:医学科学版,2004,25(z1):214-216.
16. 陈良建,黄冬梅.充气式硅橡胶赝复体修复单侧上颌骨缺损的研究[J].口腔颌面修复学杂志,2009,10(3):157-161.
17. 杜兵,陈巨峰,符志锋.计算机辅助设计与制作在磁性固位分体式上颌骨赝复体修复中的应用[J].中华口腔医学研究杂志:电子版,2011,5(2):178-183.
18. MOORE D J, GLASER Z R, TOGACOO M J, et al. Evaluation of polymeric materials for facial prosthetics[J]. J Prosthet Dent, 1977, 38(3): 319-326.
19. 赵信义.颌面赝复材料研究进展[J].国际生物医学工程杂志,2006,29(3):178-183.
20. 侯春梅,李新颖,叶伟亮,等.MTT法和CCK-8法检测悬浮细胞增殖的比较[J].军事医学科学院院刊,2009,33(4):400-402.
21. 辛培成,杨圣,芦健民,等.CCK-8法检测兔筋膜成纤维细胞增殖活性之最佳测试条件研究[J].中外医学研究,2011,9(12):6-8.

Journal of Biomedical Engineering

Cytotoxicity of a New Type Silicone Rubber for Maxillofacial Prosthesis: an In vitro Evaluation

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