Effect of Fast Capacitance Compensation Method on Improving the Action Potential Firing Accuracy of Nerve Cell_Journal of Biomedical Engineering

Authors：

ZHENGYu , LIJing , CAIDi , TIANLei , HUANGChaochao ,  WANGJinhai

School of Electronics and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China;

Corresponding author：

WANGJinhai, Email: wangjinhai@tjpu.edu.cn

Keywords：

fast capacitance; membrane capacitance; membrane potential; action potential; capacitance compensation

DOI：

10.7507/1001-5515.20140226

Video：

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Abstract Full text Figures/Tables Video References Cited by

Patch-clamp is used to study all sorts of ionic channels and their regulations with measuring pA current of cell ionic channel, but the fast capacitance (C-fast) compensation and slow capacitance (C-slow) compensation transient currents are caused by measuring objects and measuring instruments themselves which will change the properties of action potentials. The present paper firstly discusses the C-Fast transient currents affecting membrane capacitance and membrane potential, and then draws a conclusion that the changes of membrane potential affect the properties of action potential through analyzing the changes of membrane potential in H-H model. Based on this conclusion, we discuss the influence mechanisms mainly through the analysis of traditional C-fast compensation errors, and focus discussion on the shape of electrode capacitance affecting C-fast. This method can not only improve the compensation speed greatly, but also improve the compensation precision from the electrode shape as much as possible.

Citation： ZHENGYu, LIJing, CAIDi, TIANLei, HUANGChaochao, WANGJinhai. Effect of Fast Capacitance Compensation Method on Improving the Action Potential Firing Accuracy of Nerve Cell. Journal of Biomedical Engineering, 2014, 31(6): 1191-1194,1217. doi: 10.7507/1001-5515.20140226 Copy

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17.	BHARGAVA A, GORELIK J. Recording single-channel currents using "smart patch-clamp" technique[J]. Methods Mol Biol, 2013, 998:189-197.

1. SIGWORTH F J. Design of the EPC-9, a computer-controlled patch-clamp amplifier.1. Hardwar[J]. J Neurosci Methods, 1995,56(2):195-202.
2. SIGWORTH F J, AFFOLTER H, NEHER E. Design of the EPC-9, a computer-controlled patch-clamp amplifier.2. Software[J]. J Neurosci Methods, 1995,56(2):203-215.
3. 叶燚,胡刚,瞿安连.全自动膜片钳放大器快电容补偿技术改进[J].上海生物医学工程,2006,27(2):98-101.
4. 高刚强,瞿安连.膜片钳放大器瞬态电流伪差的自动补偿[J].华中科技大学学报:自然科学版,2004,32(10):63-65.
5. GILLIS K D. Single-channel recording[M]. 2nd ed. New York:Plenum Press, 1995:155-198.
6. 康国新,邹寿彬,陈良怡,等.膜片钳放大器的低噪声设计[J].电子学报,2000,28(9):42-45.
7. 李长科,徐世元,陈立学.膜片钳电生理试验干扰的来源与排除[J].第一军医大学学报,2002,22(7):656-657.
8. ZHANG H, QU A, LUO J, et al. Error analysis of Cm measurement under the whole-cell patch-clamp recording[J]. J Neurosci Methods, 2010, 185(2):307-314.
9. CHEN P, GILLIS K D. The noise of membrane capacitance measurements in the whole-cell ecording configuration[J]. Biophys J, 2000, 79(4):2162-2170.
10. MIKLAVCIC D, TOWHIDI L. Numerical study of the electroporation pulse shape effect on molecular uptake of biological cells[J]. Radiol Oncol, 2010, 44(1):34-41.
11. ZHANG H, LUO J,XIONG J, et al. Zf-and-Hsys-based Cm measurement under the whole-cell patch-clamp recording[J]. Pflugers Arch, 2009,457:1423-1434.
12. MARMARELIS Z V. Nonlinear dynamic modeling of physiological systems[M]. New Jersey:Wiley, 2004:541.
13. MARTY A, NEHER E. Neher[M]. New York:Plenum Press, 1995:31-52.
14. SAKMANN B, NEHER E. Geometric parameters of pipettes and membrane patches in single channel recording[M]. 2nd ed. New York and London:Plenum Publishing Corp, 1995:637-643.
15. 杨秀文,刘云山.玻璃介电常数的测定[J].河北大学学报:自然科学版,1995,15(2):82-85.
16. GUAN W, REED M A. Electric field modulation of the membrane potential in solid-state ion channels[J]. Nano Lett, 2012, 12(12):6441-6447.
17. BHARGAVA A, GORELIK J. Recording single-channel currents using "smart patch-clamp" technique[J]. Methods Mol Biol, 2013, 998:189-197.

Journal of Biomedical Engineering

Effect of Fast Capacitance Compensation Method on Improving the Action Potential Firing Accuracy of Nerve Cell

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