CK2β Promotes Pink1/Parkin-mediated Miro1 Degradation_Journal of Biomedical Engineering

Authors：

ZHANGChenliang , QINSiyue ,  JIANGChang

Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

JIANGChang, Email: cjcareer@qq.com

Keywords：

Parkinson's disease; CK2β; PINK1; MIRO1

DOI：

10.7507/1001-5515.20140248

Video：

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Abstract Full text Figures/Tables Video References Cited by

PTEN-induced putative kinase 1 (PINK1), a Parkinson's disease (PD)-related protein, has two isoforms, the mitochondria-localized full-length isoform PINK1FL and the cytoplasm-localized short isoform PINK1-cyto. Studies have suggested that PINK1FL can selectively accumulate at the surface of damaged mitochondria and cooperate with another Parkinson's Disease-related protein PARKIN to trigger the degradation of MIRO1, a mitochondria trafficking regulator. The functions of PINK1-cyto are, however, not yet clear. To investigate the functions of PINK1-cyto, we expressed different proteins in cultured HEK293 cells by transfecting it with different plasmids, and detected the protein levels by Western blot after expressing for 24 h. We found that in cultured HEK293 cells, PINK1-cyto could also cooperate with PARKIN degrade MIRO1 in the presence of CK2β, and the regulatory subunit of Casein Kinase Ⅱ. Interestingly, this function of CK2β was not dependent on CK2α, the catalytic subunit of Casein Kinase II. We also found that CK2β could promote the direct interaction between PINK1-cyto and MIRO1 by immunocoprecipitation analysis. This result suggested that in addition to CK2α, CK2β could also form a kinase complex with PINK1-cyto with important physiological functions.

Citation： ZHANGChenliang, QINSiyue, JIANGChang. CK2β Promotes Pink1/Parkin-mediated Miro1 Degradation. Journal of Biomedical Engineering, 2014, 31(6): 1310-1315. doi: 10.7507/1001-5515.20140248 Copy

1.	ABELIOVICH A, FLINT BEAL M. Parkinsonism genes:culprits and clues[J]. J Neurochem, 2006, 99(4):1062-1072.
2.	ABOU-SLEIMAN P M, MUQIT M M, WOOD N W. Expanding in sights of mitochondrial dysfuction in Parkinson's disease[J]. Nat Rev Neurosci, 2006, 7(3):207-219.
3.	CLARK I E, DODSON M W, JIANG C, et al. Drosophila pink1 is required for mitochondrial function and interacts genetically with Parkin[J]. Nature, 2006, 441(3):1162-1166.
4.	VIVES-BAUZA C, ZHOU C, HUANG Y, et al. PINK1-dependent recruitment of Parkin to mitochondria in mitophagy[J]. Proc Natl Acad Sci U S A, 2010, 107(1):378-383.
5.	GEGG ME, COOPERJ M, CHAU K Y, et al. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy[J]. Hum Mol Genet, 2010, 19(24):4861-4870.
6.	YANG Y, GEHRKE S, IMAI Y, et al. Mitochondrial pathology and muscle and dopaminergic neuronegeneration caused by inactivation of drosophila Pink1is rescued by pakin[J]. PNAS, 2006, 103(1):10793-10798.
7.	ZHOU C, HUANG Y, SHAO Y, et al. The kinase domain of mitochondrial PINK1 faces the cytoplasm[J]. Proc Natl Acad Sci U S A, 2008, 105(33):12022-12027.
8.	BECKER D, RICHTER J, TOCILESCU M A,et al. Pink1 kinase and its membrane potential(Deltaψ)-dependent cleavage product both localize to outer mitochondrial membrane by unique targeting mode[J]. J Biol Chem, 2012, 287(27):22969-22987.
9.	FRANSSON A, RUUSALA A, ASPENSTROM P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis[J]. J Biol Chem, 2003, 278(8):6495-6502.
10.	LIU S, SAWADA T, LEE S, et al. Parkinson's disease-associated Kinase PINK1 regulates Miro protein level and axonal transport of mitochondria[J]. PLoS Genet, 2012, 8(3):e1002537.
11.	WANG X, WINTER D, ASHRAFI G, et al. PINK1 and Parkin marget Miro for phosphorylation and degradation to arrest mitochondrial motility[J]. Cell, 2011, 147(11):893-906.
12.	GIETZ R D, GRAHAM K C, LITCHFIELD D W. Interactions between the subunits of casein kinase Ⅱ[J]. J Biol Chem, 1995, 270:1302-13021.
13.	CHEN Y, DORN G W. PINK1-phosphorylated mitofusin 2 is a Parkin receptor for culling damaged mitochondria[J]. Science, 2013, 340(6131):471-475.

1. ABELIOVICH A, FLINT BEAL M. Parkinsonism genes:culprits and clues[J]. J Neurochem, 2006, 99(4):1062-1072.
2. ABOU-SLEIMAN P M, MUQIT M M, WOOD N W. Expanding in sights of mitochondrial dysfuction in Parkinson's disease[J]. Nat Rev Neurosci, 2006, 7(3):207-219.
3. CLARK I E, DODSON M W, JIANG C, et al. Drosophila pink1 is required for mitochondrial function and interacts genetically with Parkin[J]. Nature, 2006, 441(3):1162-1166.
4. VIVES-BAUZA C, ZHOU C, HUANG Y, et al. PINK1-dependent recruitment of Parkin to mitochondria in mitophagy[J]. Proc Natl Acad Sci U S A, 2010, 107(1):378-383.
5. GEGG ME, COOPERJ M, CHAU K Y, et al. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy[J]. Hum Mol Genet, 2010, 19(24):4861-4870.
6. YANG Y, GEHRKE S, IMAI Y, et al. Mitochondrial pathology and muscle and dopaminergic neuronegeneration caused by inactivation of drosophila Pink1is rescued by pakin[J]. PNAS, 2006, 103(1):10793-10798.
7. ZHOU C, HUANG Y, SHAO Y, et al. The kinase domain of mitochondrial PINK1 faces the cytoplasm[J]. Proc Natl Acad Sci U S A, 2008, 105(33):12022-12027.
8. BECKER D, RICHTER J, TOCILESCU M A,et al. Pink1 kinase and its membrane potential(Deltaψ)-dependent cleavage product both localize to outer mitochondrial membrane by unique targeting mode[J]. J Biol Chem, 2012, 287(27):22969-22987.
9. FRANSSON A, RUUSALA A, ASPENSTROM P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis[J]. J Biol Chem, 2003, 278(8):6495-6502.
10. LIU S, SAWADA T, LEE S, et al. Parkinson's disease-associated Kinase PINK1 regulates Miro protein level and axonal transport of mitochondria[J]. PLoS Genet, 2012, 8(3):e1002537.
11. WANG X, WINTER D, ASHRAFI G, et al. PINK1 and Parkin marget Miro for phosphorylation and degradation to arrest mitochondrial motility[J]. Cell, 2011, 147(11):893-906.
12. GIETZ R D, GRAHAM K C, LITCHFIELD D W. Interactions between the subunits of casein kinase Ⅱ[J]. J Biol Chem, 1995, 270:1302-13021.
13. CHEN Y, DORN G W. PINK1-phosphorylated mitofusin 2 is a Parkin receptor for culling damaged mitochondria[J]. Science, 2013, 340(6131):471-475.

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