Effect of Gold (Au) Nanoparticles Modified by Surface Chemistry on the Proliferation and Migration of Hepatocellular Carcinoma Cells <i>in Vitro</i>_Journal of Biomedical Engineering

Authors：

HONGJinyong ¹ , YINHongmei ² , SHENYang ¹ , YANZhiping ¹ , LIUJingxia ¹ , ZHOUFating ¹ , XIAQing ¹ ,  LIUXiaoheng ¹

1. Institute of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China;
2. West China School of Pharmacy, Sichuan University, Chengdu 610041, China;

Corresponding author：

LIUXiaoheng, Email: liuxiaohg@scu.edu.cn

Keywords：

surface chemistry; gold (Au) nanoparticles; hepatocellular carcinoma cell; cell toxicity; cell migration

DOI：

10.7507/1001-5515.20150068

Video：

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Abstract Full text Figures/Tables Video References Cited by

Due to the good tumor-targeting and excellent biocompatibility, the drug-loading nanoparticles (NPs) has been widely applied in the diagnosis and treatment of cancer. However, after the NPs are recognized and internalized by cancer cells, the effects of NPs on cell migration behavior were unclear. In the present study, the self-assembly techniques (SAMs) was used to modify gold (Au) nanoparticles (Au NPs) with different chemical functional groups (CH₃, OH, COOH and NH₂) as model NPs. The dispersion of these groups in solution and the distribution in cells were studied by transmission electron microscope (TEM), respectively, and the proliferation was examined by MTT assay in vitro. The wound-healing and the Transwell assay were used to examine the effect of internalized Au-NPs on HepG2 cells migration. The results showed that different Au-NPs mainly distributed at the edge of the vesicle membrane and the gap between cells. The Au-NPs resulted in decreased cell viability in a concentration-depended manner. In addition, the results of wound-healing and Transwells assay indicated that the internalization of the NH₂-NPs and OH-NPs would inhibit cell migration compared with those in the control group.

Citation： HONGJinyong, YINHongmei, SHENYang, YANZhiping, LIUJingxia, ZHOUFating, XIAQing, LIUXiaoheng. Effect of Gold (Au) Nanoparticles Modified by Surface Chemistry on the Proliferation and Migration of Hepatocellular Carcinoma Cells in Vitro. Journal of Biomedical Engineering, 2015, 32(2): 373-379. doi: 10.7507/1001-5515.20150068 Copy

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7.	O AC'U WIEJA M, ADAMCZYK Z, MORGA M, et al. High density silver nanoparticle monolayers produced by colloid self-assembly on polyelectrolyte supporting layers[J]. J Colloid Interface Sci, 2011, 364(1):39-48.
8.	FAUCHEUX N, SCHWEISS R, LÜTZOW K, et al. Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies[J]. Biomaterials, 2004, 25(14):2721-2730.
9.	LI Di, YAN Yiming, WIECKOWSKA A, et al. Amplified electrochemical detection of DNA through the aggregation of Au nanoparticles on electrodes and the incorporation of methylene blue into the DNA-crosslinked structure[J]. Chem Commun (Camb), 2007(34):3544-3546.
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12.	PRIME K L, WHITESIDES G M. Self-assembled organic monolayers:model systems for studying adsorption of proteins at surfaces[J]. Science, 1991, 252(59):1164-1167.
13.	POCOVÍ-MARTÍNEZ S, PARREÑO-ROMERO M, AGOURAM S, et al. Controlled UV-C light-induced fusion of thiol-passivated gold nanoparticles[J]. Langmuir, 2011, 27(9):5234-5241.
14.	LÓPEZ L G, RAMIREZ-PASTOR A J. Adsorption of self-assembled rigid rods on two-dimensional lattices[J]. Langmuir, 2012, 28(42):14917-14924.
15.	PAN Yu, NEUSS S, LEIFERT A, et al. Size-dependent cytotoxicity of gold nanoparticles[J]. Small, 2007, 3(11):1941-1949.
16.	GURR J R, WANG A S, CHEN C H, et al. Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells[J]. Toxicology, 2005, 213(1-2):66-73.

1. HALEGOUA-DE MARZIO D, HANN H W. Prevention of hepatocellular carcinoma and its recurrence with anti-hepatitis B viral therapy[J]. Minerva Gastroenterol Dietol, 2014, 60(3):191-200.
2. ARⅡS, SATA M, SAKAMOTO M, et al. Management of hepatocellular carcinoma:Report of Consensus Meeting in the 45th Annual Meeting of the Japan Society of Hepatology (2009)[J]. Hepatol Res, 2010, 40(7):667-685.
3. BEDFORD E E, SPADAVECCHIA J, PRADIER C M, et al. Surface plasmon resonance biosensors incorporating Gold nanoparticles[J]. Macromol Biosci, 2012, 12(6):724-739.
4. SETYAWATI M I, TAY C Y, LEONG D T. Effect of Zinc oxide nanomaterials-induced oxidative stress on the p53 pathway[J]. Biomaterials, 2013, 34(38):10133-10142.
5. SETYAWATI M I, TAY C Y, CHIA S L, et al. Titanium dioxide nanomaterials cause endothelial cell leakiness by disrupting the homophilic interaction of VE-cadherin[J]. Nat Commun, 2013, 4:1673-1679.
6. BOULOS S P, PRIGOZHIN M B, LIU Yuan, et al. The Gold Nanorod-Biology interface:from proteins to cells to tissue[J]. Curr Phys Chem, 2013, 3(2):657-665.
7. O AC'U WIEJA M, ADAMCZYK Z, MORGA M, et al. High density silver nanoparticle monolayers produced by colloid self-assembly on polyelectrolyte supporting layers[J]. J Colloid Interface Sci, 2011, 364(1):39-48.
8. FAUCHEUX N, SCHWEISS R, LÜTZOW K, et al. Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies[J]. Biomaterials, 2004, 25(14):2721-2730.
9. LI Di, YAN Yiming, WIECKOWSKA A, et al. Amplified electrochemical detection of DNA through the aggregation of Au nanoparticles on electrodes and the incorporation of methylene blue into the DNA-crosslinked structure[J]. Chem Commun (Camb), 2007(34):3544-3546.
10. ITOH H, TAHARA A, NAKA K, et al. Photochemical assembly of gold nanoparticles utilizing the photodimerization of thymine[J]. Langmuir, 2004, 20(5):1972-1976.
11. LAM A T, YOON J, GANBOLD E O, et al. Colloidal gold nanoparticle conjugates of gefitinib[J]. Colloids Surf B Biointerfaces, 2014, 3(2):124-132.
12. PRIME K L, WHITESIDES G M. Self-assembled organic monolayers:model systems for studying adsorption of proteins at surfaces[J]. Science, 1991, 252(59):1164-1167.
13. POCOVÍ-MARTÍNEZ S, PARREÑO-ROMERO M, AGOURAM S, et al. Controlled UV-C light-induced fusion of thiol-passivated gold nanoparticles[J]. Langmuir, 2011, 27(9):5234-5241.
14. LÓPEZ L G, RAMIREZ-PASTOR A J. Adsorption of self-assembled rigid rods on two-dimensional lattices[J]. Langmuir, 2012, 28(42):14917-14924.
15. PAN Yu, NEUSS S, LEIFERT A, et al. Size-dependent cytotoxicity of gold nanoparticles[J]. Small, 2007, 3(11):1941-1949.
16. GURR J R, WANG A S, CHEN C H, et al. Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells[J]. Toxicology, 2005, 213(1-2):66-73.

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Effect of Gold (Au) Nanoparticles Modified by Surface Chemistry on the Proliferation and Migration of Hepatocellular Carcinoma Cells in Vitro

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