Controlled Release of Low Molecular Protein Insulin-like Growth Factor-1 through Self-Assembling Peptide Hydrogel with Biotin Sandwich Approach_Journal of Biomedical Engineering

Authors：

 LIUYanfei , FANZhenhai , WANGYuying , YULimei

Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medcial College, Zunyi 563003, China;

Corresponding author：

LIUYanfei, Email: l_yfei@163.com

Keywords：

biotin sandwich approach; self-assembling peptide hydrogel; protein release; insulin-like growth factor 1

DOI：

10.7507/1001-5515.20150070

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Abstract Full text Figures/Tables Video References Cited by

Since the release rate of protein in hydrogels is directly dependent upon the size of the protein and the hydrogel, how to deliver low molecular weight protein for prolonged periods has always been a problem. In this article, we present a usage of self-assembling peptide (P3) with the RGD epitope on its N terminus. The concentration of the released insulin-like growth factor 1 (IGF-1) was determined by UV-vis spectroscopy and the release kinetics suggested a notable reduction of the IGF-1 release rate. Cell entrapment experiments revealed that IGF-1 delivery by biotinylated nanofibers could promote the proliferation of the mouse chondrogenic ATDC5 cells when compared with cells embedded within nanofibers with untethered IGF-1.

Citation： LIUYanfei, FANZhenhai, WANGYuying, YULimei. Controlled Release of Low Molecular Protein Insulin-like Growth Factor-1 through Self-Assembling Peptide Hydrogel with Biotin Sandwich Approach. Journal of Biomedical Engineering, 2015, 32(2): 387-392. doi: 10.7507/1001-5515.20150070 Copy

1.	陈咏竹, 邱峰, 赵晓军.自组装短肽系统及其材料学应用[J].材料科学与工程学报, 2009, 27(2):292-296.
2.	DE LA RICA R, MATSUI H. Applications of peptide and protein-based materials in bionanotechnology[J]. Chem Soc Rev, 2010, 39(9):3499-3509.
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5.	刘燕飞, 吴敏, 刘博, 等.自组装短肽水凝胶对功能性蛋白质IGF-1、aFGF以及VEGF的缓释[J].生物医学工程学杂志, 2011, 28(2):310-313.
6.	WALLACE D G, ROSENBLATT J. Collagen gel systems for sustained delivery and tissue engineering[J]. Adv Drug Deliv Rev, 2003, 55(12):1631-1649.
7.	ZISCH A H, SCHENK U, SCHENSE J C, et al. Covalently conjugated VEGF--fibrin matrices for endothelialization[J]. J Control Release, 2001, 72(1):101-113.
8.	VERHEYEN E, DELAIN-BIOTON L, DER WAL S V, et al. Protein macromonomers for covalent immobilization and subsequent triggered release from hydrogels[J]. J Control Release, 2010, 148(1):e18-e19.
9.	CENSI R, DI MARTINO P, VERMONDEN T, et al. Hydrogels for protein delivery in tissue engineering[J]. Journal of Controlled Release, 2012, 161(2):680-692.
10.	SAKIYAMA-ELBERT S E, HUBBELL J A. Development of fibrin derivatives for controlled release of heparin-binding growth factors[J]. J Control Release, 2000, 65(3):389-402.
11.	SCHILLEMANS J P, HENNINK W E, VAN NOSTRUM C F. Charged dextran hydrogels for post-loading and release of proteins[J]. J Control Release, 2010, 148(1):e82-e83.
12.	DE WOLF F A, BRETT G M. Ligand-binding proteins:their potential for application in systems for controlled delivery and uptake of ligands[J]. Pharmacol Rev, 2000, 52(2):207-236.
13.	SARGEANT T D, GULER M O, OPPENHEIMER S M, et al. Hybrid bone implants:self-assembly of peptide amphiphile nanofibers within porous Titanium[J]. Biomaterials, 2008, 29(2):161-171.
14.	MILLER R E, KOPESKY P W, GRODZINSKY A J. Growth factor delivery through self-assembling peptide scaffolds[J]. Clin Orthop Relat Res, 2011, 469(10):2716-2724.
15.	DAVIS M E, HSIEH P C, TAKAHASHI T, et al. Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction[J]. Proc Natl Acad Sci U S A, 2006, 103(21):8155-8160.
16.	PADIN-IRUEGAS M E, MISAO Y, DAVIS M E, et al. Cardiac progenitor cells and biotinylated insulin-like growth factor-1 nanofibers improve endogenous and exogenous myocardial regeneration after infarction[J]. Circulation, 2009, 120(10):876-887.
17.	LIU Y F, ZHAO X J. PRESENTATION OF BIOACTIVE EPITOPES WITH FREE N-TERMINI ON SELF-ASSEMBLING PEPTIDE NANOFIBERS[J]. Nano, 2011, 6(1):47-57.
18.	焦利敏, 廖学品, 石碧.紫外分光光度法下直接测定蛋白质溶液的浓度[J].化学研究与应用, 2007, 19(5):562-566.
19.	LAVIK E, LANGER R. Tissue engineering:current state and perspectives[J]. Appl Microbiol Biotechnol, 2004, 65(1):1-8.
20.	HOFFMAN A S. Hydrogels for biomedical applications[J]. Adv Drug Deliv Rev, 2002, 54(1):3-12.
21.	PEPPAS N A, HILT J Z, KHADEMHOSSEINI A, et al. Hydrogels in biology and medicine:From molecular principles to bionanotechnology[J]. Adv Mater, 2006, 18(11):1345-1360.

1. 陈咏竹, 邱峰, 赵晓军.自组装短肽系统及其材料学应用[J].材料科学与工程学报, 2009, 27(2):292-296.
2. DE LA RICA R, MATSUI H. Applications of peptide and protein-based materials in bionanotechnology[J]. Chem Soc Rev, 2010, 39(9):3499-3509.
3. KOUTSOPOULOS S, UNSWORTH L D, NAGAI Y, et al. Controlled release of functional proteins through designer self-assembling peptide nanofiber hydrogel scaffold[J]. Proc Natl Acad Sci U S A, 2009, 106(12):4623-4628.
4. BRANCO M C, POCHAN D J, WAGNER N J, et al. The effect of protein structure on their controlled release from an injectable peptide hydrogel[J]. Biomaterials, 2010, 31(36):9527-9534.
5. 刘燕飞, 吴敏, 刘博, 等.自组装短肽水凝胶对功能性蛋白质IGF-1、aFGF以及VEGF的缓释[J].生物医学工程学杂志, 2011, 28(2):310-313.
6. WALLACE D G, ROSENBLATT J. Collagen gel systems for sustained delivery and tissue engineering[J]. Adv Drug Deliv Rev, 2003, 55(12):1631-1649.
7. ZISCH A H, SCHENK U, SCHENSE J C, et al. Covalently conjugated VEGF--fibrin matrices for endothelialization[J]. J Control Release, 2001, 72(1):101-113.
8. VERHEYEN E, DELAIN-BIOTON L, DER WAL S V, et al. Protein macromonomers for covalent immobilization and subsequent triggered release from hydrogels[J]. J Control Release, 2010, 148(1):e18-e19.
9. CENSI R, DI MARTINO P, VERMONDEN T, et al. Hydrogels for protein delivery in tissue engineering[J]. Journal of Controlled Release, 2012, 161(2):680-692.
10. SAKIYAMA-ELBERT S E, HUBBELL J A. Development of fibrin derivatives for controlled release of heparin-binding growth factors[J]. J Control Release, 2000, 65(3):389-402.
11. SCHILLEMANS J P, HENNINK W E, VAN NOSTRUM C F. Charged dextran hydrogels for post-loading and release of proteins[J]. J Control Release, 2010, 148(1):e82-e83.
12. DE WOLF F A, BRETT G M. Ligand-binding proteins:their potential for application in systems for controlled delivery and uptake of ligands[J]. Pharmacol Rev, 2000, 52(2):207-236.
13. SARGEANT T D, GULER M O, OPPENHEIMER S M, et al. Hybrid bone implants:self-assembly of peptide amphiphile nanofibers within porous Titanium[J]. Biomaterials, 2008, 29(2):161-171.
14. MILLER R E, KOPESKY P W, GRODZINSKY A J. Growth factor delivery through self-assembling peptide scaffolds[J]. Clin Orthop Relat Res, 2011, 469(10):2716-2724.
15. DAVIS M E, HSIEH P C, TAKAHASHI T, et al. Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction[J]. Proc Natl Acad Sci U S A, 2006, 103(21):8155-8160.
16. PADIN-IRUEGAS M E, MISAO Y, DAVIS M E, et al. Cardiac progenitor cells and biotinylated insulin-like growth factor-1 nanofibers improve endogenous and exogenous myocardial regeneration after infarction[J]. Circulation, 2009, 120(10):876-887.
17. LIU Y F, ZHAO X J. PRESENTATION OF BIOACTIVE EPITOPES WITH FREE N-TERMINI ON SELF-ASSEMBLING PEPTIDE NANOFIBERS[J]. Nano, 2011, 6(1):47-57.
18. 焦利敏, 廖学品, 石碧.紫外分光光度法下直接测定蛋白质溶液的浓度[J].化学研究与应用, 2007, 19(5):562-566.
19. LAVIK E, LANGER R. Tissue engineering:current state and perspectives[J]. Appl Microbiol Biotechnol, 2004, 65(1):1-8.
20. HOFFMAN A S. Hydrogels for biomedical applications[J]. Adv Drug Deliv Rev, 2002, 54(1):3-12.
21. PEPPAS N A, HILT J Z, KHADEMHOSSEINI A, et al. Hydrogels in biology and medicine:From molecular principles to bionanotechnology[J]. Adv Mater, 2006, 18(11):1345-1360.

Journal of Biomedical Engineering

Controlled Release of Low Molecular Protein Insulin-like Growth Factor-1 through Self-Assembling Peptide Hydrogel with Biotin Sandwich Approach

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