Research Progress in Seeding Cells of Peripheral Nerve_Journal of Biomedical Engineering

Authors：

 SHIGengqiang , HUYi

School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;

Corresponding author：

SHIGengqiang, Email: gengersgq@163.com

Keywords：

seeding cell; Schwann cell; bone marrow mesenchymal stem cell; neural stem cell

DOI：

10.7507/1001-5515.20150085

Video：

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Abstract Full text Figures/Tables Video References Cited by

Seeding cells play an important role in the peripheral nerve damage repair. Seeding cells studied consequently in peripheral nerve are Schwann cells, bone marrow mesenchymal stem cells and neural stem cells. Schwann cells, the first seeding cells, are various unique glial cells in the peripheral nervous system, which can form the myelin sheath for insulation and package of the neuron projecting axons in the peripheral nervous system so that the conduction velocity of the nerve signal was accelerated. It can be proved that Schwann cells played an important role in the maintenance of peripheral nerve function and in the regeneration process after peripheral nerve injury. The second, bone marrow mesenchymal stem cells are the various mesenchymal stem cells mainly exist in the systemic connective tissues and organs. These functional stem cells are often studied at present, and it has been found that they have exuberant proliferation and differentiation potentials. Neural stem cells, mentioned the third in sequence, are the kind of pluripotent cells with multi-directional differentiation, which could conduct the self-renewal function, and generate and differentiate neurons, astrocytes and oligodendrocytes through asymmetric cell division. These three types of seed cells are discussed in this paper.

Citation： SHIGengqiang, HUYi. Research Progress in Seeding Cells of Peripheral Nerve. Journal of Biomedical Engineering, 2015, 32(2): 470-474. doi: 10.7507/1001-5515.20150085 Copy

1.	TORIGOE K, TANAKA H F, TAKAHASHI A, et al. Basic behavior of migratory Schwann cells in peripheral nerve regeneration[J]. Exp Neurol, 1996, 137(2):301-308.
2.	付红运, 王锐英.雪旺细胞体外培养与纯化的研究进展[J].海南医学, 2012, 23(22):118-121.
3.	TYNER T R, PARKS N, FARIA S, et al. Effects of collagen nerve guide on neuroma formation and neuropathic pain in a rat model[J]. Am J Surg, 2007, 193(1):e1-e6.
4.	NAVARRO X, VIVÓ M, VALERO-CABRÉ A. Neural plasticity after peripheral nerve injury and regeneration[J]. Prog Neurobiol, 2007, 82(4):163-201.
5.	成玉泽, 李剑波, 石敏, 等.成人及成年大鼠雪旺细胞的体外培养[J].江苏医药, 2011, 37(17):1985-1987.
6.	陶晓宇, 滕晓华, 刘波, 等.改良新生大鼠雪旺细胞的体外培养和纯化[J].湖南师范大学自然科学学报, 2011, 34(3):82-84, 89.
7.	王洪美, 胡琴, 姜晓荣, 等.三种人胚胎许旺细胞培养方法的比较[J].中华临床医师杂志(电子版), 2011, 5(19):5599-5603.
8.	陈祥, 赵明, 周华山, 等.新生大鼠雪旺细胞原代培养方法改良[J].四川动物, 2012, 31(3):464-467.
9.	王世清, 范志海, 沈忆新.不同部位取材的雪旺细胞培养与纯化研究[J].中国修复重建外科杂志, 2011, 25(2):166-170.
10.	KRAUS A, TÄGER J, KOHLER K, et al. Efficacy of various durations of in vitro predegeneration on the cell count and purity of rat Schwann-cell cultures[J]. J Neurotrauma, 2010, 27(1):197-203.
11.	王耀军, 郑朝, 张战凤, 等.成年大鼠许旺细胞快速扩增的改良方法[J].中华临床医师杂志(电子版), 2010, 4(12):2538-2541.
12.	张华, 时淑艳, 刘佳, 等.一种简便的雪旺细胞培养方法[J].四川解剖学杂志, 2012, 20(1):7-9.
13.	KOMIYAMA T, NAKAO Y, TOYAMA Y, et al. A novel technique to isolate adult Schwann cells for an artificial nerve conduit[J]. J Neurosci Methods, 2003, 122(2):195-200.
14.	秦义武, 赵劲民, 苏伟, 等.单酶消化复合植块法培养雪旺细胞的实验研究[J].中国修复重建外科杂志, 2012, 26(9):1107-1111.
15.	刘炎, 张莉, 宫旭, 等.双向差速法制备高纯度雪旺细胞的实验研究[J].中国矫形外科杂志, 2011, 19(16):1373-1376.
16.	李岩峰.激活态雪旺细胞临床应用安全性的实验研究[D].上海:复旦大学, 2010.
17.	YANG Q, MU J, LI Q, et al. A simple and efficient method for deriving neurospheres from bone marrow stromal cells[J]. Biochem Biophys Res Commun, 2008, 372(4):520-524.
18.	曹敏丽, 赵红斌, 吕志成.超声评价骨髓间充质干细胞在外周神经缺损修复中的价值[J].临床误诊误治, 2011, 24(12):4-5.
19.	MIMURA T, DEZAWA M, KANNO H, et al. Peripheral nerve regeneration by transplantation of bone marrow stromal cell-derived Schwann cells in adult rats[J]. J Neurosurg, 2004, 101(5):806-812.
20.	SHIMIZU S, KITADA M, ISHIKAWA H, et al. Peripheral nerve regeneration by the in vitro differentiated-human bone marrow stromal cells with Schwann cell property[J]. Biochem Biophys Res Commun, 2007, 359(4):915-920.
21.	郑煜, 周兴.神经细胞移植促进大鼠损伤的周围神经功能的恢复[J].实用医学杂志, 2012, 28(20):3359-3361.
22.	张杭, 孙天威.骨髓间充质干细胞分化为神经细胞研究进展[J].国际骨科学杂志, 2011, 32(2):104-107.
23.	安秀峰, 黄汉昌, 姜招峰.骨髓间充质干细胞向神经干细胞的分化[J].生命科学, 2013, 25(1):84-90.
24.	HU N, WU H, XUE C B, et al. Long-term outcome of the repair of 50 mm long median nerve defects in rhesus monkeys with marrow mesenchymal stem cells-containing, chitosan-based tissue engineered nerve grafts[J]. Biomaterials, 2013, 34(1):100-111.
25.	ZHENG L, CUI H F. Enhancement of nerve regeneration along a chitosan conduit combined with bone marrow mesenchymal stem cells[J]. J Mater Sci Mater Med, 2012, 23(9):2291-2302.
26.	MIRA H, ANDREU Z, SUH H, et al. Signaling through BMPR-IA regulates quiescence and long-term activity of neural stem cells in the adult hippocampus[J]. Cell Stem Cell, 2010, 7(1):78-89.
27.	NUMANO F, INOUE A, ENOMOTO M, et al. Critical involvement of Rho GTPase activity in the efficient transplantation of neural stem cells into the injured spinal cord[J]. Mol Brain, 2009, 2:37.
28.	PASPALA S A, BALAJI A B, NYAMATH P, et al. Neural stem cells & supporting cells--the new therapeutic tools for the treatment of spinal cord injury[J]. Indian J Med Res, 2009, 130(4):379-391.
29.	KEIRSTEAD H S, BEN-HUR T, ROGISTER B, et al. Polysialylated neural cell adhesion molecule-positive CNS precursors generate both oligodendrocytes and Schwann cells to remyelinate the CNS after transplantation[J]. J Neurosci, 1999, 19(17):7529-7536.
30.	MURAKAMI T, FUJIMOTO Y, YASUNAGA Y, et al. Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair[J]. Brain Res, 2003, 974(1-2):17-24.
31.	CHANG K A, KIM J W, KIM J A, et al. Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells[J]. PLoS One, 2011, 6(4):e18738.
32.	张民皓, 胡益民, 张红, 等.坐骨神经损伤大鼠模型鞘内移植神经干细胞后脊髓背角和背根神经节脑源性神经营养因子的表达[J].中国组织工程研究, 2012, 16(10):1851-1855.
33.	CHENG L N, DUAN X H, ZHONG X M, et al. Transplanted neural stem cells promote nerve regeneration in acute peripheral nerve traction injury:assessment using MRI[J]. AJR Am J Roentgenol, 2011, 196(6):1381-1387.
34.	XU L, ZHOU S, FENG G Y, et al. Neural stem cells enhance nerve regeneration after sciatic nerve injury in rats[J]. Mol Neurobiol, 2012, 46(2):265-274.
35.	GU S H, XU W D, XU L, et al. Regenerated host axons form synapses with neurons derived from neural stem cells transplanted into peripheral nerves[J]. J Int Med Res, 2012, 38(5):1721-1729.

1. TORIGOE K, TANAKA H F, TAKAHASHI A, et al. Basic behavior of migratory Schwann cells in peripheral nerve regeneration[J]. Exp Neurol, 1996, 137(2):301-308.
2. 付红运, 王锐英.雪旺细胞体外培养与纯化的研究进展[J].海南医学, 2012, 23(22):118-121.
3. TYNER T R, PARKS N, FARIA S, et al. Effects of collagen nerve guide on neuroma formation and neuropathic pain in a rat model[J]. Am J Surg, 2007, 193(1):e1-e6.
4. NAVARRO X, VIVÓ M, VALERO-CABRÉ A. Neural plasticity after peripheral nerve injury and regeneration[J]. Prog Neurobiol, 2007, 82(4):163-201.
5. 成玉泽, 李剑波, 石敏, 等.成人及成年大鼠雪旺细胞的体外培养[J].江苏医药, 2011, 37(17):1985-1987.
6. 陶晓宇, 滕晓华, 刘波, 等.改良新生大鼠雪旺细胞的体外培养和纯化[J].湖南师范大学自然科学学报, 2011, 34(3):82-84, 89.
7. 王洪美, 胡琴, 姜晓荣, 等.三种人胚胎许旺细胞培养方法的比较[J].中华临床医师杂志(电子版), 2011, 5(19):5599-5603.
8. 陈祥, 赵明, 周华山, 等.新生大鼠雪旺细胞原代培养方法改良[J].四川动物, 2012, 31(3):464-467.
9. 王世清, 范志海, 沈忆新.不同部位取材的雪旺细胞培养与纯化研究[J].中国修复重建外科杂志, 2011, 25(2):166-170.
10. KRAUS A, TÄGER J, KOHLER K, et al. Efficacy of various durations of in vitro predegeneration on the cell count and purity of rat Schwann-cell cultures[J]. J Neurotrauma, 2010, 27(1):197-203.
11. 王耀军, 郑朝, 张战凤, 等.成年大鼠许旺细胞快速扩增的改良方法[J].中华临床医师杂志(电子版), 2010, 4(12):2538-2541.
12. 张华, 时淑艳, 刘佳, 等.一种简便的雪旺细胞培养方法[J].四川解剖学杂志, 2012, 20(1):7-9.
13. KOMIYAMA T, NAKAO Y, TOYAMA Y, et al. A novel technique to isolate adult Schwann cells for an artificial nerve conduit[J]. J Neurosci Methods, 2003, 122(2):195-200.
14. 秦义武, 赵劲民, 苏伟, 等.单酶消化复合植块法培养雪旺细胞的实验研究[J].中国修复重建外科杂志, 2012, 26(9):1107-1111.
15. 刘炎, 张莉, 宫旭, 等.双向差速法制备高纯度雪旺细胞的实验研究[J].中国矫形外科杂志, 2011, 19(16):1373-1376.
16. 李岩峰.激活态雪旺细胞临床应用安全性的实验研究[D].上海:复旦大学, 2010.
17. YANG Q, MU J, LI Q, et al. A simple and efficient method for deriving neurospheres from bone marrow stromal cells[J]. Biochem Biophys Res Commun, 2008, 372(4):520-524.
18. 曹敏丽, 赵红斌, 吕志成.超声评价骨髓间充质干细胞在外周神经缺损修复中的价值[J].临床误诊误治, 2011, 24(12):4-5.
19. MIMURA T, DEZAWA M, KANNO H, et al. Peripheral nerve regeneration by transplantation of bone marrow stromal cell-derived Schwann cells in adult rats[J]. J Neurosurg, 2004, 101(5):806-812.
20. SHIMIZU S, KITADA M, ISHIKAWA H, et al. Peripheral nerve regeneration by the in vitro differentiated-human bone marrow stromal cells with Schwann cell property[J]. Biochem Biophys Res Commun, 2007, 359(4):915-920.
21. 郑煜, 周兴.神经细胞移植促进大鼠损伤的周围神经功能的恢复[J].实用医学杂志, 2012, 28(20):3359-3361.
22. 张杭, 孙天威.骨髓间充质干细胞分化为神经细胞研究进展[J].国际骨科学杂志, 2011, 32(2):104-107.
23. 安秀峰, 黄汉昌, 姜招峰.骨髓间充质干细胞向神经干细胞的分化[J].生命科学, 2013, 25(1):84-90.
24. HU N, WU H, XUE C B, et al. Long-term outcome of the repair of 50 mm long median nerve defects in rhesus monkeys with marrow mesenchymal stem cells-containing, chitosan-based tissue engineered nerve grafts[J]. Biomaterials, 2013, 34(1):100-111.
25. ZHENG L, CUI H F. Enhancement of nerve regeneration along a chitosan conduit combined with bone marrow mesenchymal stem cells[J]. J Mater Sci Mater Med, 2012, 23(9):2291-2302.
26. MIRA H, ANDREU Z, SUH H, et al. Signaling through BMPR-IA regulates quiescence and long-term activity of neural stem cells in the adult hippocampus[J]. Cell Stem Cell, 2010, 7(1):78-89.
27. NUMANO F, INOUE A, ENOMOTO M, et al. Critical involvement of Rho GTPase activity in the efficient transplantation of neural stem cells into the injured spinal cord[J]. Mol Brain, 2009, 2:37.
28. PASPALA S A, BALAJI A B, NYAMATH P, et al. Neural stem cells & supporting cells--the new therapeutic tools for the treatment of spinal cord injury[J]. Indian J Med Res, 2009, 130(4):379-391.
29. KEIRSTEAD H S, BEN-HUR T, ROGISTER B, et al. Polysialylated neural cell adhesion molecule-positive CNS precursors generate both oligodendrocytes and Schwann cells to remyelinate the CNS after transplantation[J]. J Neurosci, 1999, 19(17):7529-7536.
30. MURAKAMI T, FUJIMOTO Y, YASUNAGA Y, et al. Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair[J]. Brain Res, 2003, 974(1-2):17-24.
31. CHANG K A, KIM J W, KIM J A, et al. Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells[J]. PLoS One, 2011, 6(4):e18738.
32. 张民皓, 胡益民, 张红, 等.坐骨神经损伤大鼠模型鞘内移植神经干细胞后脊髓背角和背根神经节脑源性神经营养因子的表达[J].中国组织工程研究, 2012, 16(10):1851-1855.
33. CHENG L N, DUAN X H, ZHONG X M, et al. Transplanted neural stem cells promote nerve regeneration in acute peripheral nerve traction injury:assessment using MRI[J]. AJR Am J Roentgenol, 2011, 196(6):1381-1387.
34. XU L, ZHOU S, FENG G Y, et al. Neural stem cells enhance nerve regeneration after sciatic nerve injury in rats[J]. Mol Neurobiol, 2012, 46(2):265-274.
35. GU S H, XU W D, XU L, et al. Regenerated host axons form synapses with neurons derived from neural stem cells transplanted into peripheral nerves[J]. J Int Med Res, 2012, 38(5):1721-1729.

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Research Progress in Seeding Cells of Peripheral Nerve

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