A Canine Portal Hypertension Model Induced by Intra-portal Administration of Polyurethane-Tetrahydrofuran Solutions_Journal of Biomedical Engineering

Authors：

YANXiaopeng ^1,2,3 , RENFenggang ^1,2,3 , MAJia ⁴ , DONGDinghui ^1,2,3 , XUEFei ^1,2,3 ,  LUYi ^1,2,3

1. Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China;
2. XJTU Research Institute of Advanced Surgical Technology and Engineering, Xi'an Jiaotong University, Xi'an 710061, China;
3. Regenerative Medicine and Surgery Engineering Research Center of Shaanxi Province, Xi'an 710061, China;
4. Oncological Surgery, Shaanxi Provincial People's Hospital, Xi'an 710068, China;

Corresponding author：

LUYi, Email: luyi169@126.com

Keywords：

portal hypertension; animal model; polyurethane; dog

DOI：

10.7507/1001-5515.20150117

Video：

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This study was to build a canine portal hypertension model by intra-portal administration of high polymer material polyurethane and organic solvent tetrahydrofuran mixed solutions in order to evaluate the effectiveness of the model. Twelve local crossbreed dogs were selected randomly, with intra-portal administration of 8% (weight/volume) polyurethane-tetrahydrofuran solutions through an incision in the upper abdomen to build the portal hypertension model. We measured the portal vein pressure before modeling, during modeling, and four-, eight-, and twelve-weeks after modeling, respectively. Then we evaluated the effectiveness of the model comparing values of data with those data obtained before modeling started, which were regarded as the normal values. The results showed that the portal vein pressure rose by 2.5 times after the solution administrated instantly as much as that before modeling, and maintained at 1.5 times after 4 weeks. This method presents an easy operation, low animal mortality and reliable model of portal hypertension. Its less abdominal adhesions and its ability in keeping normal anatomic structure specially make it suit for surgical research of portal hypertension.

Citation： YANXiaopeng, RENFenggang, MAJia, DONGDinghui, XUEFei, LUYi. A Canine Portal Hypertension Model Induced by Intra-portal Administration of Polyurethane-Tetrahydrofuran Solutions. Journal of Biomedical Engineering, 2015, 32(3): 645-649. doi: 10.7507/1001-5515.20150117 Copy

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1. 张日平, 张伟辉, 薛东波, 等.四氯化碳诱导犬早期肝损害所致门静脉高压的形态学观察[J].中华医学杂志, 2004, 84(13):66-69.
2. 陈钟, 戴向华, 明志祥, 等.改良四氯化碳皮下注射法制备犬肝硬化门静脉高压症模型[J].中国普通外科杂志, 2009, 18(1):63-67.
3. TSUKAMOTO H, MATSUOKA M, FRENCH S W. Experimental models of hepatic fibrosis:a review[J]. Semin Liver Dis, 1990, 10(1):56-65.
4. 张谊, 张启瑜, 廖毅, 等.硫代乙酰胺剂量个体化诱导大鼠肝硬化门脉高压模型[J].肝胆胰外科杂志, 2007, 19(3):164-167.
5. 赵莉, 李振东, 于增文, 等.肝前型门静脉高压症动物(犬)模型制作体会[J].中华小儿外科杂志, 2001, 22(1):42-44.
6. 池信锦, 葛缅, 黄品婕, 等.犬门脉高压模型的构建[J].中山大学学报:医学科学版, 2008, 29(S1):4-5.
7. 李宗芳, 刘效恭, 王英, 等.缩窄门静脉主干1/2加丝线慢性栓塞术制备犬门静脉高压症模型[J].中华实验外科杂志, 1997, 14(5):314-315.
8. 章来长, 廖瑾愉, 李卫东, 等.白芨粉栓塞门静脉制备犬门脉高压症模型的实验研究[J].航空军医, 1994, 22(1):19-21.
9. 叶乐驰, 杨文军, 殷薇薇, 等.纱布团栓塞门静脉制备犬门脉高压症模型[J].中华实验外科杂志, 2008, 25(3):389-390.
10. 陈军, 吴性江, 黎介寿.门静脉内注射葡聚糖微球制成猪门静脉高压症模型[J].中华实验外科杂志, 2005, 22(6):755-756.
11. SHI L B, PENG C H, PENG S Y, et al. Preliminary experimental study on treatment of portal hypertension with auxiliary partial orthotopic live transplantation[J].中国危重病急救医学, 2004, 16(12):730-733.
12. 鲍俊杰, 刘都宝, 黎兵, 等.医用聚氨酯材料研究进展[J].聚氨酯, 2007, 64(9):72-79.

Journal of Biomedical Engineering

A Canine Portal Hypertension Model Induced by Intra-portal Administration of Polyurethane-Tetrahydrofuran Solutions

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