Inhibitory Effect of Hydroxyapatite Particles with Different Size on Malignant Melanoma A375 Cells: A Preliminary Study_Journal of Biomedical Engineering

Authors：

GUOBo ^1,2 , LIBo ² , WANGYan ³ , HONGYouliang ² , ZHANGLingli ² ,  ZHANGXingdong ²

1. Department of Ophthalmology, West China Hospital of Sichuan University, Chengdu 610041, China;
2. National Biomedical Materials Engineering Technology Research Center, Sichuan University, Chengdu 610064, China;
3. Department of Obstetrics and Gynecology, Sichuan Provincial People's Hospital, Chengdu 610072, China;

Corresponding author：

ZHANGXingdong, Email: zhangxd@scu.edu.cn

Keywords：

hydroxyapatite particle; malignant melanoma; nanometer

DOI：

10.7507/1001-5515.20150150

Video：

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To explore the inhibitory effect of hydroxyapatite (HA) particles with different sizes on malignant melanoma A375 cells in vitro, we synthesized 4 short rod-like HA particles using TIPS. Their mean diameters were 998.0 nm (HA1), 511.0 nm (HA2), 244.0 nm (HA3), and 71.6 nm (HA4), respectively. Malignant melanoma A375 cells were co-cultured with HA particles in vitro. Results showed that HA particles smaller than 511.0 nm in mean diameter could always inhibit proliferation of A375 cells, and nanometer-HA particles (HA4) had the strongest inhibitory effect on A375 cell proliferation and the strongest inducing effect on apoptosis. HA particles were distributed in plasma of A375 cells. The ultrastructure changes of A375 cells were found most significant in nanometer-HA particles (HA4) group. We conclude that particle size is a very important influencing factor on anti-tumor effects of HA and that nanometer-HA particle has the strongest inhibitory effect on tumor cell proliferation.

Citation： GUOBo, LIBo, WANGYan, HONGYouliang, ZHANGLingli, ZHANGXingdong. Inhibitory Effect of Hydroxyapatite Particles with Different Size on Malignant Melanoma A375 Cells: A Preliminary Study. Journal of Biomedical Engineering, 2015, 32(4): 832-837. doi: 10.7507/1001-5515.20150150 Copy

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1. AOKI H,AKAO M,KANO S.Effects of HAP-sol on cell growth[J].Report of the institute for Medical and Dental Engineering,1992,26(1):15-21.
2. KANO S,YAMAZAKI A,OTSUKA R,et al.Application of hydroxyapatite-sol as drug carrier[J].Biomed Mater Eng,1994,4(4):283-290.
3. SUN Jiao,XUE Miao,KIKUCHI M,et al.Effects of Sr-hydroxyapatite microcrystal on cultured cell[J].Biomed Mater Eng,1994,4(7):503-512.
4. LAQUERRIERE P,GRANDJEAN-LAQUERRIERE A,ADDADI-REBBAH S,et al.MMP-2,MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles[J].Biomaterials,2004,25(13):2515-2524.
5. JIN Jun,ZUO Gui-fu,XIONG Guang-yao,et al.The inhibition of lamellar hydroxyapatite and lamellar magnetic hydroxyapatite on the migration and adhesion of breast cancer cells[J].J Mater Sci Mater Med,2014,25(4):1025-1031.
6. EZHAVENI S,YUVAKKUMAR R,RAJKUMAR M A,et al.Preparation and characterization of nano-hydroxyapatite nanomaterials for liver cancer cell treatment[J].J Nanosci Nanotechnol,2013,13(3):1631-1638.
7. QING Fangzhu,WANG Zhe,HONG Youliang,et al.Selective effects of hydroxyapatite nanoparticles on osteosarcoma cells and osteoblasts[J].J Mater Sci Mater Med,2012,23(9):2245-2251.
8. 李基文.21世纪纳米技术在医学应用中的展望[J].中国公共卫生,2001,17(8):717-718.
9. YEH T C,ZHANG W,ILDSTAD S T,et al.Intracellular labeling of T-cells with superparamagnetic contrast agents[J].Magn Reson Med,1993,30(5):617-625.
10. LUO Yun,LING You,GUO Wu-sheng,et al.Docetaxel loaded oleic acid-coated hydroxyapatite nanoparticles enhance the docetaxel-induced apoptosis through activation of caspase-2 in androgen independent prostate cancer cells[J].J Control Release,2010,147(2):278-288.
11. XU Jing,XU Pengjuan,LI Zhigui,et al.Oxidative stress and apoptosis induced by hydroxyapatite nanoparticles in C6 cells[J].J Biomed Mater Res A,2012,100(3):738-745.
12. CHEN Xiao-juan,DENG Chang-sheng,TANG Sheng-li,et al.Mitochondria-dependent apoptosis induced by nanoscale hydroxyapatite in human gastric cancer SGC-7901 cells[J].Biol Pharm Bull,2007,30(1):128-132.
13. ZHANG Mingjun,LIU Shengnan,XU Ge,et al.Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells[J].Int J Nanomedicine,2014,9(1):265-271.

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Inhibitory Effect of Hydroxyapatite Particles with Different Size on Malignant Melanoma A375 Cells: A Preliminary Study

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