Effect of Chit-oligosaccharide/Collagen Composite Gels on Pre-osteoblastic MC3T3-E1s Differentiation_Journal of Biomedical Engineering

Authors：

WENJianhua ¹ , LIZhenzhen ¹ , JIAWeijian ¹ ,  DINGShan ^1,2 , ZHOUChangren ^1,2

1. Department of Materials Science and Engineering, Jinan University, Guangzhou 510630, China;
2. Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510630, China;

Corresponding author：

DINGShan, Email: tdingshan@jnu.edu.cn

Keywords：

chit-oligosaccharide; collagen; hydrogel; osteogenic differentiation

DOI：

10.7507/1001-5515.20160083

Video：

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Abstract Full text Figures/Tables Video References Cited by

Chit-oligosaccharide (COS) is a low-molecular, water-soluble mass with higher biological activity, which can be absorbed by human body easily and interact with cells directly. Based on the excellent biological properties of collagen (Col) and COS, a series of Col and COS composite hydrogel (Col/COSn) was constructed in this study. The effect of composite hydrogel on cells proliferation, differentiation and related osteogenic gene expression was evaluated on pre-osteoblast MC3T3-E1s. The experimental results showed that all the Col/COS composite gels could promote the growth of MC3T3-E1s, proliferation and bone related gene expression compared to that of pure Col gels. And there was significant difference among the composite hydrogel groups with different degrees of polymerization of COS. The effect of the composite gel which contained chitotetraose (COS4) or chitohexaose (COS6) on the cells proliferation was better than that of other groups, while on cells differentiation and related osteogenic gene expression the composite gel contained chitopentaose (COS5) was the best in all the groups.

Citation： WENJianhua, LIZhenzhen, JIAWeijian, DINGShan, ZHOUChangren. Effect of Chit-oligosaccharide/Collagen Composite Gels on Pre-osteoblastic MC3T3-E1s Differentiation. Journal of Biomedical Engineering, 2016, 33(3): 493-498. doi: 10.7507/1001-5515.20160083 Copy

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1. XU Yi, GURUSIDDAPPA S, RICH R L, et al. Multiple binding sites in collagen type Ⅰ for the integrins α1β1 and α2β1[J]. Journal of Biological Chemistry, 2000, 275(50):38981-38989.
2. JOKINEN J, DADU E, NYKVIST P, et al. Integrin-mediated cell adhesion to type Ⅰ collagen fibrils[J]. Journal of Biological Chemistry, 2004, 279(30):31956-31963.
3. SALASZNYK R M, WILLIAMS W A, BOSKEY A, et al. Adhesion to vitronectin and collagen Ⅰ promotes osteogenic differentiation of human mesenchymal stem cells[J]. J Biomed Biotechnol, 2004, 2004(1):24-34.
4. KUNDU A K, PUTNAM A J. Vitronectin and collagen Ⅰ differentially regulate osteogenesis in mesenchymal stem cells[J]. Biochem Biophys Res Commun, 2006, 347(1):347-357.
5. KIM S K, RAJAPAKSE N. Enzymatic production and biological activities of chitosan oligosaccharides (COS):A review[J]. Carbohydr Polym, 2005, 62(4):357-368.
6. DANG Yibing, LI Sheng, WANG Wenxia, et al. The effects of chitosan oligosaccharide on the activation of murine spleen CD11c⁺ dendritic cells via Toll-like receptor 4[J]. Carbohydr Polym, 2011, 83(3):1075-1081.
7. JIANG Maorong, ZHUGE X, YANG Yumin, et al. The promotion of peripheral nerve regeneration by chitooligosaccharides in the rat nerve crush injury model[J]. Neurosci Lett, 2009, 454(3):239-243.
8. YANG Yumin, LIU Mei, GU Yun, et al. Effect of chitooligosaccharide on neuronal differentiation of PC-12 cells[J]. Cell Biol Int, 2009, 33(3):352-356.
9. 蒋茂荣,诸葛晓,丁斐.壳寡糖促进小鼠坐骨神经再生的实验研究[J].苏州大学学报医学版,2010,30(1):4-6,37.
10. JUNG W K, MOON S H, KIM S K. Effect of chitooligosaccharides on calcium bioavailability and bone strength in ovariectomized rats[J]. Life Sci, 2006, 78(9):970-976.
11. SHACKLETON M, VAILLANT F, SIMPSON K J, et al. Generation of a functional mammary gland from a single stem cell[J]. Nature, 2006, 439(772):84-88.
12. MIZUNO M, KUBOKI Y. Osteoblast-related gene expression of bone marrow cells during the osteoblastic differentiation induced by type Ⅰ collagen[J]. J Biochem, 2001, 129(1):133-138.
13. LEE N K, SOWA H, HINOI E, et al. Endocrine regulation of energy metabolism by the skeleton[J]. Cell, 2007, 130(3):456-469.
14. STEIN G S, LIAN J B. Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype[J]. Endocrine Reviews, 1993, 14(4):424-442.
15. RATANAVARAPORN J,KANOKPANONT S,TABATA Y, et al. Growth and osteogenic differentiation of adipose-derived and bone marrow-derived stem cells on chitosan and chitooligosaccharide films[J]. Carbohydrate Polymers, 2009, 78(4):873-878.

Journal of Biomedical Engineering

Effect of Chit-oligosaccharide/Collagen Composite Gels on Pre-osteoblastic MC3T3-E1s Differentiation

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