Effect of lung ischemia-reperfusion injury on the expressions of transient receptor potential vanilloid 1 in lung and brainstem of rats_Journal of Biomedical Engineering

Authors：

WANG Wenjian ¹ , CHENG Yan ² ,  WANG Rurong ²

1. Department of Anesthesiology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R.China;
2. Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China;

Corresponding author：

WANG Rurong, Email: rurongwang@foxmail.com

Keywords：

lung ischemia reperfusion injury; transient receptor potential vanilloid 1; calcitonin gene-related peptide; substance P

DOI：

10.7507/1001-5515.201601042

Video：

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This study aims to investigate the effect of lung ischemia reperfusion injury (LIRI) on expression of transient receptor potential vanilloid 1 (TRPV1) in the lung and brainstem of rats. Sixteen adult male Sprague Dawley rats weighing 250-320 g were randomly divided into Sham group and ischemia reperfusion group (IR group). Before ischemia, 0.5 hour and 4 hours after the reperfusion, respectively, arterial partial pressure of oxygen (PaO₂) and arterial-alveolar oxygen pressure gradient (A-aDO₂) were recorded and calculated, respectively. Left lung tissues and the brainstems were obtained at the end of the experiment. Lung tissue malondialdehyde (MDA), myeloperoxidase (MPO) activities, calcitonin gene related peptide (CGRP) and substance P (SP) levels were assessed. The mRNA and protein expressions of TRPV1 in the lung and brainstem were measured by qRT-PCR and Western blot. Compared with in the Sham group, rats in the IR group had a poorer blood gas exchange (P<0.05) and the MPO activity and MDA level of lung tissues in the IR group were significantly higher than those in the Sham group (P<0.05). CGRP level in the IR group increased remarkably (P<0.05), while SP level did not differ statistically between the two groups (P>0.05). The mRNA and protein expressions of TRPV1 in the lung tissue were upregulated in the IR group (P<0.05), but there were no differences of those in the brainstem between the two groups (P>0.05). The results suggest that LIRI could upregulate the expressions of TRPV1 and evoke CGRP release in the lung.

Citation： WANGWenjian, CHENGYan, WANGRurong. Effect of lung ischemia-reperfusion injury on the expressions of transient receptor potential vanilloid 1 in lung and brainstem of rats. Journal of Biomedical Engineering, 2017, 34(1): 87-91. doi: 10.7507/1001-5515.201601042 Copy

1.	Watanabe N, Horie S, Michael G J, et al. Immunohistochemical localization of vanilloid receptor subtype 1 (TRPV1) in the guinea pig respiratory system. Pulm Pharmacol Ther, 2005, 18(3): 187-197.
2.	Geraghty D P, Mazzone S B, Carter C A. Effects of systemic capsaicin treatment on TRPV1 and tachykinin NK1 receptor distribution and function in the nucleus of the solitary tract of the adult rat. Pharmacology, 2011, 87(3/4): 214-223.
3.	Lennerz J K, Rühle V, Ceppa E P, et al. Calcitonin receptor-like receptor (CLR), receptor activity-modifying protein 1 (RAMP1), and calcitonin gene-related peptide (CGRP) immunoreactivity in the rat trigeminovascular system: differences between peripheral and central CGRP receptor distribution. J Comp Neurol, 2008, 507(3): 1277-1299.
4.	Hagner S, Stahl U, Knoblauch B, et al. Calcitonin receptor-like receptor: identification and distribution in human peripheral tissues. Cell Tissue Res, 2002, 310(1): 41-50.
5.	Ma W, Chabot J G, Powell K J, et al. Localization and modulation of calcitonin gene-related peptide-receptor component protein-immunoreactive cells in the rat central and peripheral nervous systems. Neuroscience, 2003, 120(3): 677-694.
6.	Wang Maohua, Ji Peng, Wang Rurong, et al. TRPV1 agonist capsaicin attenuates lung ischemia-reperfusion injury in rabbits. J Surg Res, 2012, 173(1): 153-160.
7.	Ji Peng, Jiang Tao, Wang Maohua, et al. Denervation of capsaicin-sensitive C fibers increases pulmonary inflammation induced by ischemia-reperfusion in rabbits. J Surg Res, 2013, 184(2): 782-789.
8.	Wang L H, Wang D H. TRPV1 gene knockout impairs postischemic recovery in isolated perfused heart in mice. Circulation, 2005, 112(23): 3617-3623.
9.	Tohda C, Sasaki M, Konemura T, et al. Axonal transport of VR1 capsaicin receptor mRNA in primary afferents and its participation in inflammation-induced increase in capsaicin sensitivity. J Neurochem, 2001, 76(6): 1628-1635.
10.	Mazzone S B, Geraghty D P. Respiratory action of capsaicin microinjected into the nucleus of the solitary tract: involvement of vanilloid and tachykinin receptors. Br J Pharmacol, 1999, 127(2): 473-481.
11.	丁晓慧, 苏秋香, 吴敏范, 等. 脊髓电刺激对大鼠心肌缺血 SP 和 TRPV1 表达的影响. 解剖科学进展, 2009, 15(2): 179-182.
12.	Gao Yuanfeng, Song Junxian, Chen Hong, et al. TRPV1 activation is involved in the cardioprotection of remote limb ischemic postconditioning in ischemia-reperfusion injury rats. Biochem Biophys Res Commun, 2015, 463(4): 1034-1039.
13.	Sakamoto K, Kuroki T, Okuno Y, et al. Activation of the TRPV1 channel attenuates N-methyl-D-aspartic acid-induced neuronal injury in the rat retina. Eur J Pharmacol, 2014, 733: 13-22.
14.	Zheng Liangrong, Zhang Yuanyuan, Han Jie, et al. Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor. J Diabetes Complications, 2015, 29(3): 323-328.

1. Watanabe N, Horie S, Michael G J, et al. Immunohistochemical localization of vanilloid receptor subtype 1 (TRPV1) in the guinea pig respiratory system. Pulm Pharmacol Ther, 2005, 18(3): 187-197.
2. Geraghty D P, Mazzone S B, Carter C A. Effects of systemic capsaicin treatment on TRPV1 and tachykinin NK1 receptor distribution and function in the nucleus of the solitary tract of the adult rat. Pharmacology, 2011, 87(3/4): 214-223.
3. Lennerz J K, Rühle V, Ceppa E P, et al. Calcitonin receptor-like receptor (CLR), receptor activity-modifying protein 1 (RAMP1), and calcitonin gene-related peptide (CGRP) immunoreactivity in the rat trigeminovascular system: differences between peripheral and central CGRP receptor distribution. J Comp Neurol, 2008, 507(3): 1277-1299.
4. Hagner S, Stahl U, Knoblauch B, et al. Calcitonin receptor-like receptor: identification and distribution in human peripheral tissues. Cell Tissue Res, 2002, 310(1): 41-50.
5. Ma W, Chabot J G, Powell K J, et al. Localization and modulation of calcitonin gene-related peptide-receptor component protein-immunoreactive cells in the rat central and peripheral nervous systems. Neuroscience, 2003, 120(3): 677-694.
6. Wang Maohua, Ji Peng, Wang Rurong, et al. TRPV1 agonist capsaicin attenuates lung ischemia-reperfusion injury in rabbits. J Surg Res, 2012, 173(1): 153-160.
7. Ji Peng, Jiang Tao, Wang Maohua, et al. Denervation of capsaicin-sensitive C fibers increases pulmonary inflammation induced by ischemia-reperfusion in rabbits. J Surg Res, 2013, 184(2): 782-789.
8. Wang L H, Wang D H. TRPV1 gene knockout impairs postischemic recovery in isolated perfused heart in mice. Circulation, 2005, 112(23): 3617-3623.
9. Tohda C, Sasaki M, Konemura T, et al. Axonal transport of VR1 capsaicin receptor mRNA in primary afferents and its participation in inflammation-induced increase in capsaicin sensitivity. J Neurochem, 2001, 76(6): 1628-1635.
10. Mazzone S B, Geraghty D P. Respiratory action of capsaicin microinjected into the nucleus of the solitary tract: involvement of vanilloid and tachykinin receptors. Br J Pharmacol, 1999, 127(2): 473-481.
11. 丁晓慧, 苏秋香, 吴敏范, 等. 脊髓电刺激对大鼠心肌缺血 SP 和 TRPV1 表达的影响. 解剖科学进展, 2009, 15(2): 179-182.
12. Gao Yuanfeng, Song Junxian, Chen Hong, et al. TRPV1 activation is involved in the cardioprotection of remote limb ischemic postconditioning in ischemia-reperfusion injury rats. Biochem Biophys Res Commun, 2015, 463(4): 1034-1039.
13. Sakamoto K, Kuroki T, Okuno Y, et al. Activation of the TRPV1 channel attenuates N-methyl-D-aspartic acid-induced neuronal injury in the rat retina. Eur J Pharmacol, 2014, 733: 13-22.
14. Zheng Liangrong, Zhang Yuanyuan, Han Jie, et al. Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor. J Diabetes Complications, 2015, 29(3): 323-328.

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Effect of lung ischemia-reperfusion injury on the expressions of transient receptor potential vanilloid 1 in lung and brainstem of rats

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