Experimental Comparison Research between Two Kinds of Modified Poly (lactic acid) Material <i>In Vitro</i>_Journal of Biomedical Engineering

Authors：

WANGLingling , CUIZhiming ,  XUGuanhua , LIWeidong , BAOGuofeng , SUNYuyu , ZHANGJinlong

Department of Spinal Surgery, the Second Affiliated Hospital of Nantong University, Nantong 226001, China;

Corresponding author：

XUGuanhua, Email: xghspine@163.com

Keywords：

poly (lactic acid); poly (glycolic acid); chitosan; olfactory ensheathing cells; biocompatibility

DOI：

10.7507/1001-5515.20160147

Video：

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This study aims to compare two kinds of modified poly (lactic acid) (PLA) materials:PLA-chitosan (PLA-CTS) and PLA-poly (glycolic acid) (PLA-PGA). PLA-CTS and PLA-PGA scaffolds were prepared and observed under electron microscope. The scaffold porosity was calculated and the pH of the degradation solution was measured. Then rat olfactory ensheathing cells (OECs) were cultivated, and mixed cultured respectively with two scaffolds as two groups. The proliferation, adhesion rate and growth condition of the OECs were observed and compared between the two groups. Results showed that both the prepared PLA-CTS and PLA-PGA scaffolds were three-dimensional porous structure and the porosity of PLA-CTS was 91%, while that of PLA-PGA was 87%. The pH of degradation solution decreased gradually, of which PLA-PGA fell faster than PLA-CTS. After added to the two scaffolds, most OECs could grow well, and there were no significant differences between the two groups on MTT test and nuclei number determined by fluorescent microscope. However, the cell adhesion rate of PLA-CTS group was significantly higher than that of PLA-PGA. It can be concluded that compared with PLA-PGA, PLA-CTS might be a better choice as OECs scaffold.

Citation： WANG Lingling, CUI Zhiming, XU Guanhua, LI Weidong, BAO Guofeng, SUN Yuyu, ZHANG Jinlong. Experimental Comparison Research between Two Kinds of Modified Poly (lactic acid) Material In Vitro. Journal of Biomedical Engineering, 2016, 33(5): 911-915. doi: 10.7507/1001-5515.20160147 Copy

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14.	XIA Lei, WAN Hong, HAO Shuyu, et al. Co-transplantation of neural stem cells and Schwann cells within poly (L-lactic-co-glycolic acid) scaffolds facilitates axonal regeneration in hemisected rat spinal cord[J]. Chin Med J (Engl), 2013, 126(5):909-917.
15.	ZHENG Lei, CUI Huifei. Enhancement of nerve regeneration along a chitosan conduit combined with bone marrow mesenchymal stem cells[J]. J Mater Sci Mater Med, 2012, 23(9):2291-2302.
16.	YI Xin, JIN G, TIAN Meiling, et al. Porous chitosan scaffold and NGF promote neuronal differentiation of neural stem cells in vitro[J]. Neuro Endocrinol Lett, 2011, 32(5):705-710.
17.	王征, 刘万顺, 韩宝芹, 等.不同分子量羧甲基壳聚糖的制备及其对皮肤成纤维细胞和角质形成细胞生长的影响[J].生物医学工程学杂志, 2007, 24(2):340-344.
18.	LI Xiaoming, LIU Xinhui, DONG Wei, et al. In vitro evaluation of porous poly(L-lactic acid) scaffold reinforced by chitin fibers[J]. J Biomed Mater Res B Appl Biomater, 2009, 90B(2):503-509.
19.	SHALUMON K T, SATHISH D, NAIR S V, et al. Fabrication of aligned poly(lactic acid)-chitosan nanofibers by novel parallel blade collector method for skin tissue engineering[J]. J Biomed Nanotechnol, 2012, 8(3):405-416.
20.	ZHANG Zhe, CUI Huifei. Biodegradability and biocompatibility study of poly(chitosan-g-lactic acid) scaffolds[J]. Molecules, 2012, 17(3):3243-3258.

1. BOCCAFOSCHI F, FUSARO L, MOSCA C, et al. The biological response of poly(L-lactide) films modified by different biomolecules:role of the coating strategy[J]. J Biomed Mater Res A, 2012, 100A(9):2373-2381.
2. HUANG W C, YAO C K, LIAO J D, et al. Enhanced Schwann cell adhesion and elongation on a topographically and chemically modified poly(L-lactic acid) film surface[J]. J Biomed Mater Res A, 2011, 99A(2):158-165.
3. THARION G, INDIRANI K, DURAI M, et al. Motor recovery following olfactory ensheathing cell transplantation in rats with spinal cord injury[J]. Neurol India, 2011, 59(4):566-572.
4. LOPES A. Olfactory ensheathing cells for human spinal cord injury[J]. Neurorehabil Neural Repair, 2010, 24(8):772-773.
5. 崔颖, 崔志明, 徐冠华, 等.PLGA与大鼠嗅鞘细胞的生物相容性研究[J].组织工程与重建外科杂志, 2011, 7(2):80-84.
6. 衣昕, 金国华, 田美玲, 等.壳聚糖支架与神经干细胞生物相容性的研究[J].神经解剖学杂志, 2007, 23(5):506-510.
7. POTTER W, KALIL R E, KAO W J. Biomimetic material systems for neural progenitor cell-based therapy[J]. Front Biosci, 2008, 13:806-821.
8. CHEN B K, KNIGHT A M, DE RUITER G C, et al. Axon regeneration through scaffold into distal spinal cord after transection[J]. J Neurotrauma, 2009, 26(10):1759-1771.
9. KUBINOVÁ S, SYKOVÁ E. Biomaterials combined with cell therapy for treatment of spinal cord injury[J]. Regen Med, 2012, 7(2):207-224.
10. JIN Lin, FENG Zhangqi, ZHU Meiling, et al. A novel fluffy conductive polypyrrole nano-layer coated PLLA fibrous scaffold for nerve tissue engineering[J]. J Biomed Nanotechnol, 2012, 8(5):779-785.
11. DI TORO R, BETTI V, SPAMPINATO S. Biocompatibility and integrin-mediated adhesion of human osteoblasts to poly(DL-lactide-co-glycolide) copolymers[J]. Eur J Pharm Sci, 2004, 21(2/3):161-169.
12. NOJEHDEHIAN H, MOZTARZADEH F, BAHARVAND H, et al. Effect of poly-L-lysine coating on retinoic acid-loaded PLGA microspheres in the differentiation of carcinoma stem cells into neural cells[J]. Int J Artif Organs, 2010, 33(10):721-730.
13. MOORE M J, FRIEDMAN J A, LEWELLYN E B, et al. Multiple-channel scaffolds to promote spinal cord axon regeneration[J]. Biomaterials, 2006, 27(3):419-429.
14. XIA Lei, WAN Hong, HAO Shuyu, et al. Co-transplantation of neural stem cells and Schwann cells within poly (L-lactic-co-glycolic acid) scaffolds facilitates axonal regeneration in hemisected rat spinal cord[J]. Chin Med J (Engl), 2013, 126(5):909-917.
15. ZHENG Lei, CUI Huifei. Enhancement of nerve regeneration along a chitosan conduit combined with bone marrow mesenchymal stem cells[J]. J Mater Sci Mater Med, 2012, 23(9):2291-2302.
16. YI Xin, JIN G, TIAN Meiling, et al. Porous chitosan scaffold and NGF promote neuronal differentiation of neural stem cells in vitro[J]. Neuro Endocrinol Lett, 2011, 32(5):705-710.
17. 王征, 刘万顺, 韩宝芹, 等.不同分子量羧甲基壳聚糖的制备及其对皮肤成纤维细胞和角质形成细胞生长的影响[J].生物医学工程学杂志, 2007, 24(2):340-344.
18. LI Xiaoming, LIU Xinhui, DONG Wei, et al. In vitro evaluation of porous poly(L-lactic acid) scaffold reinforced by chitin fibers[J]. J Biomed Mater Res B Appl Biomater, 2009, 90B(2):503-509.
19. SHALUMON K T, SATHISH D, NAIR S V, et al. Fabrication of aligned poly(lactic acid)-chitosan nanofibers by novel parallel blade collector method for skin tissue engineering[J]. J Biomed Nanotechnol, 2012, 8(3):405-416.
20. ZHANG Zhe, CUI Huifei. Biodegradability and biocompatibility study of poly(chitosan-g-lactic acid) scaffolds[J]. Molecules, 2012, 17(3):3243-3258.

Journal of Biomedical Engineering

Experimental Comparison Research between Two Kinds of Modified Poly (lactic acid) Material In Vitro

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