Self-assembling peptide GFS-4 nanofiber scaffolds for three-dimensional cell cultures and myocardial infarction repair_Journal of Biomedical Engineering

Authors：

CHEN Zhenyin ¹ , YUE Yuanyuan ¹ , ZHANG Huinan ¹ , CHEN Chunyan ² , LIU Bo ³ , LI Xueqin ⁴ , WEN Jing ¹ , WU Shuyi ¹ ,  LUO Zhongli ¹

1. The Molecular Medicine and Cancer Research Center, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, P.R.China;
2. The Department of Cardiac, People's Hospital of Zhengzhou, Zhengzhou 450000, P.R.China;
3. The Department of Cardiac, Xin Hua Affiliated Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R.China;
4. The Department of Laboratory Medicine, Leshan Maternal and Child Health Hospital, Leshan, Sichuan 614000, P.R.China;

Corresponding author：

LUO Zhongli, Email: Zhongliluo@163.com

Keywords：

self-assembling peptide; GFS-4; three-dimensional cell cultures; myocardial infarction repair

DOI：

10.7507/1001-5515.201605061

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The purpose of this study is to investigate the effects of self-assembling peptide GFS-4 on three-dimen-sional myocardial cell culture and tissue repair of myocardial infarction. The circular dichroism (CD) spectrum was used to detect secondary structure of GFS-4, and atomic force microscope (AFM) was used to analyze the microstructure of self-assembly. The nanofiber scaffolds self-assembled by GFS-4 were used as the three-dimensional culture material to observe the growth effect of cardiomyocytes. The model of myocardial infarction was established and the effect of GFS-4 on myocardial infarction was studied. The results indicated that self-assembling peptide GFS-4 could form mainly β-sheet structure that can form dense nanofiber scaffolds after 24 hours’ self-assembling. The myocardial cells had a favorable growth status in GFS-4 nanofiber scaffold hydrogel when cells treated in three-dimen-sional cell culture. The experiment of repairing myocardial infarction in vitro proved that peptide GFS-4 hydrogel scaffold could alleviate tissue necrosis in a myocardial infarction area. As a new nanofiber scaffold material, self-assembling peptide GFS-4 can be used for three-dimensional cell culture and tissue repairing in myocardial infarction area.

Citation： CHEN Zhenyin, YUE Yuanyuan, ZHANG Huinan, CHEN Chunyan, LIU Bo, LI Xueqin, WEN Jing, WU Shuyi, LUO Zhongli. Self-assembling peptide GFS-4 nanofiber scaffolds for three-dimensional cell cultures and myocardial infarction repair. Journal of Biomedical Engineering, 2017, 34(3): 388-393. doi: 10.7507/1001-5515.201605061 Copy

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6.	Luo Zhongli, Yue Yuanyuan, Zhang Yanfang, et al. Designer D-form self-assembling peptide nanofiber scaffolds for 3-dimensional cell cultures. Biomaterials, 2013, 34(21): 4902-4913.
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8.	Liu Xi, Wang Xiumei, Wang Xiujuan, et al. Functionalized self-assembling peptide nanofiber hydrogels mimic stem cell niche to control human adipose stem cell behavior in vitro. Acta Biomater, 2013, 9(6): 6798-6805.
9.	Mabry K M, Payne S Z, Anseth K S. Microarray analyses to quantify advantages of 2D and 3D hydrogel culture systems in maintaining the native valvular interstitial cell phenotype. Biomaterials, 2016, 74: 31-41.
10.	Cheng Ke, Malliaras K, Shen Deliang, et al. Intramyocardial injection of platelet gel promotes endogenous repair and augments cardiac function in rats with myocardial infarction. J Am Coll Cardiol, 2012, 59(3): 256-264.

1. Beltrami A P, Barlucchi L, Torella D, et al. Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell, 2003, 114(6): 763-776.
2. Sanada F, Kim J, Czarna A, et al. c-Kit-positive cardiac stem cells nested in hypoxic niches are activated by stem cell factor reversing the aging myopathy. Circ Res, 2014, 114(1): 41-55.
3. Fernández-Muiños T, Recha-Sancho L, López-Chicón P, et al. Bimolecular based heparin and self-assembling hydrogel for tissue engineering applications. Acta Biomater, 2015, 16: 35-48.
4. Prat-Vidal C, Gálvez-Montón C, Puig-Sanvicens V, et al. Online monitoring of myocardial bioprosthesis for cardiac repair. Int J Cardiol, 2014, 174(3): 654-661.
5. Luo Zhongli, Zhang Shuguang. Designer nanomaterials using chiral self-assembling peptide systems and their emerging benefit for society. Chem Soc Rev, 2012, 41(13): 4736-4754.
6. Luo Zhongli, Yue Yuanyuan, Zhang Yanfang, et al. Designer D-form self-assembling peptide nanofiber scaffolds for 3-dimensional cell cultures. Biomaterials, 2013, 34(21): 4902-4913.
7. Boopathy A V, Che P L, Somasuntharam I, et al. The modulation of cardiac progenitor cell function by hydrogel-dependent Notch1 activation. Biomaterials, 2014, 35(28): 8103-8112.
8. Liu Xi, Wang Xiumei, Wang Xiujuan, et al. Functionalized self-assembling peptide nanofiber hydrogels mimic stem cell niche to control human adipose stem cell behavior in vitro. Acta Biomater, 2013, 9(6): 6798-6805.
9. Mabry K M, Payne S Z, Anseth K S. Microarray analyses to quantify advantages of 2D and 3D hydrogel culture systems in maintaining the native valvular interstitial cell phenotype. Biomaterials, 2016, 74: 31-41.
10. Cheng Ke, Malliaras K, Shen Deliang, et al. Intramyocardial injection of platelet gel promotes endogenous repair and augments cardiac function in rats with myocardial infarction. J Am Coll Cardiol, 2012, 59(3): 256-264.

Journal of Biomedical Engineering

Self-assembling peptide GFS-4 nanofiber scaffolds for three-dimensional cell cultures and myocardial infarction repair

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