Influence of Fe<sub>2</sub>O<sub>3</sub> nanoparticles on the rhological properties of simulated asthma airway mucus _Journal of Biomedical Engineering

Authors：

WANG Jiali ¹ ,  LUO Mingzhi ¹ , LIU Zhiwei ¹ , JIN Yang ³ , LIANG Shuai ² , LIU Lei ¹ , JIANG Xingmao ² , DENG Linhong ^1,3

1. Changzhou Key Laboratory of Respiratory Medical Engineering, Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu 213164, P.R.China;
2. School of petrochemical engineering, Changzhou University, Changzhou, Jiangsu 213164, P.R.China;
3. Key Laboratory of Biorheological Science and Technology, Ministry of Education, Chongqing University, Chongqing 400030, P.R.China;

Corresponding author：

LUO Mingzhi, Email: luomingzhi@cczu.edu.cn

Keywords：

asthma; Fe₂O₃ nanoparticles; airway mucus; rheology

DOI：

10.7507/1001-5515.201608001

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

The properties of mucus in a person with asthma can alter with disease process so that it may lead to the airway embolism. Fe₂O₃ nanoparticles can be used for drug delivery. Up till now, however, little is known about how the Fe₂O₃ nanoparticles influence the properties of airway mucus. In this study, Fe₂O₃ nanoparticles were dispersed with ultrasound, and the morphological properties were measured with scanning electron microscope, atomic force microscope and nanometer laser particle size and zeta potential analyzer. Then the dispersed Fe₂O₃ nanoparticles were added to the simulated asthma airway mucus with different final concentration (0.03, 0.3, and 0.4 mg/mL). The measurements of flow curve, yield stress, large amplitude oscillatory shear (LAOS) and shock scanning were carried out with a rotational rheometer. Experimental results showed that the Fe₂O₃ nanoparticles reduced the zero shear viscosity of simulated asthma airway mucus. With increase of shear rate, the wind speed of mucus was reduced. The yield stress of simulated asthma airway mucus was 19.0 Pa, but the yield stresses of experimental group (0.03, 0.3 and 0.4 mg/mL) were 17.0, 0.99, and 0.7 Pa, respectively. The results showed that the viscoelastic modulus of asthma airway mucus treated with Fe₂O₃ nanoparticles were changed obviously as measured with large amplitude scanning and frequency scanning. By adopting the method of optical phase microscopy, we found that different structures of simulated airway mucus were absorbed. The results showed Fe₂O₃ nanoparticles distroyed mucus structure. The experimental results proved that Fe₂O₃ nanoparticles could change the rheological characteristics of simulated asthma airway mucus. This experimental result would lay a foundation for the further development of airway mucus sticky agent based on the function of Fe₂O₃ nanoparticles.

Citation： WANG Jiali, LUO Mingzhi, LIU Zhiwei, JIN Yang, LIANG Shuai, LIU Lei, JIANG Xingmao, DENG Linhong. Influence of Fe₂O₃ nanoparticles on the rhological properties of simulated asthma airway mucus . Journal of Biomedical Engineering, 2017, 34(2): 193-199. doi: 10.7507/1001-5515.201608001 Copy

1.	Papatriantafyllou M. The dual mucus defence. Nat Rev Immunol, 2013, 13(11): 774.
2.	Button B, CAI L H, Ehre C, et al. A periciliary brush promotes the lung health by separating the mucus layer from airway epithelia. Science, 2012, 337(697): 937-941.
3.	Fahy J V, Dickey B F. Airway mucus function and dysfunction. N Engl J Med, 2010, 363(23): 2233-2247.
4.	Lai S K, Wang Yingying, Wirtz D, et al. Micro- and macrorheology of mucus. Adv Drug Deliv Rev, 2009, 61(2): 86-100.
5.	Johnson D C. Airway mucus function and dysfunction. N Engl J Med, 2011, 364(10): 978; author reply 978.
6.	Kim W D. Lung mucus: a clinician's view. Eur Respir J, 1997, 10(8): 1914-1917.
7.	Ohbayashi H, Setoguchi Y, Fukuchi Y, et al. Pharmacological effects of lysozyme on COPD and bronchial asthma with sputum: A randomized, placebo-controlled, small cohort, cross-over study. Pulm Pharmacol Ther, 2016, 37: 73-80.
8.	Nordgård C, Nonstad U, Olderøy M, et al. Alterations in mucus barrier function and matrix structure induced by guluronate oligomers. Biomacromolecules, 2014, 15(6): 2294-2300.
9.	Hamed R, Fiegel J. Synthetic tracheal mucus with native rheological and surface tension properties. J Biomed Mater Res A, 2014, 102(6): 1788-1798.
10.	Dickey B F. Walking on solid ground. Science, 2012, 337(697): 924-925.
11.	Thornton D, Sheehan J. From mucins to mucus: toward a more coherent understanding of this essential barrier. Proc Am Thorac Soc, 2004, 1(1): 54-61.
12.	Volsko T. Airway clearance therapy: finding the evidence. Respir Care, 2013, 58(10): 1669-1678.
13.	Tomaiuolo G, Rusciano G, Caserta S, et al. A new method to improve the clinical evaluation of cystic fibrosis patients by mucus viscoelastic properties. PLoS One, 2014, 9(1): e82297.
14.	Vasquez E S, Bowser J, Swiderski C A, et al. Rheological characterization of mammalian lung mucus. RSC Adv, 2014, 4(66): 34780-34783.
15.	Mcgill S L, Smyth H D C. Disruption of the mucus barrier by topically applied exogenous particles. Mol Pharm, 2010, 7(6): 2280-2288.
16.	Daviskas E, Rubin B K. Effect of inhaled dry powder mannitol on mucus and its clearance. Expert Rev Respir Med, 2013, 7(1): 65-75.
17.	Chen E Y T, Wang Y C, Chen C S, et al. Functionalized positive nanoparticles reduce mucin swelling and dispersion// Aiche Meeting, 2010: e15434.
18.	Wang Yingying, Lai S K, So C, et al. Mucoadhesive nanoparticles May disrupt the protective human mucus barrier by altering its microstructure. PLoS One, 2011, 6(6): e21547.

1. Papatriantafyllou M. The dual mucus defence. Nat Rev Immunol, 2013, 13(11): 774.
2. Button B, CAI L H, Ehre C, et al. A periciliary brush promotes the lung health by separating the mucus layer from airway epithelia. Science, 2012, 337(697): 937-941.
3. Fahy J V, Dickey B F. Airway mucus function and dysfunction. N Engl J Med, 2010, 363(23): 2233-2247.
4. Lai S K, Wang Yingying, Wirtz D, et al. Micro- and macrorheology of mucus. Adv Drug Deliv Rev, 2009, 61(2): 86-100.
5. Johnson D C. Airway mucus function and dysfunction. N Engl J Med, 2011, 364(10): 978; author reply 978.
6. Kim W D. Lung mucus: a clinician's view. Eur Respir J, 1997, 10(8): 1914-1917.
7. Ohbayashi H, Setoguchi Y, Fukuchi Y, et al. Pharmacological effects of lysozyme on COPD and bronchial asthma with sputum: A randomized, placebo-controlled, small cohort, cross-over study. Pulm Pharmacol Ther, 2016, 37: 73-80.
8. Nordgård C, Nonstad U, Olderøy M, et al. Alterations in mucus barrier function and matrix structure induced by guluronate oligomers. Biomacromolecules, 2014, 15(6): 2294-2300.
9. Hamed R, Fiegel J. Synthetic tracheal mucus with native rheological and surface tension properties. J Biomed Mater Res A, 2014, 102(6): 1788-1798.
10. Dickey B F. Walking on solid ground. Science, 2012, 337(697): 924-925.
11. Thornton D, Sheehan J. From mucins to mucus: toward a more coherent understanding of this essential barrier. Proc Am Thorac Soc, 2004, 1(1): 54-61.
12. Volsko T. Airway clearance therapy: finding the evidence. Respir Care, 2013, 58(10): 1669-1678.
13. Tomaiuolo G, Rusciano G, Caserta S, et al. A new method to improve the clinical evaluation of cystic fibrosis patients by mucus viscoelastic properties. PLoS One, 2014, 9(1): e82297.
14. Vasquez E S, Bowser J, Swiderski C A, et al. Rheological characterization of mammalian lung mucus. RSC Adv, 2014, 4(66): 34780-34783.
15. Mcgill S L, Smyth H D C. Disruption of the mucus barrier by topically applied exogenous particles. Mol Pharm, 2010, 7(6): 2280-2288.
16. Daviskas E, Rubin B K. Effect of inhaled dry powder mannitol on mucus and its clearance. Expert Rev Respir Med, 2013, 7(1): 65-75.
17. Chen E Y T, Wang Y C, Chen C S, et al. Functionalized positive nanoparticles reduce mucin swelling and dispersion// Aiche Meeting, 2010: e15434.
18. Wang Yingying, Lai S K, So C, et al. Mucoadhesive nanoparticles May disrupt the protective human mucus barrier by altering its microstructure. PLoS One, 2011, 6(6): e21547.

Previous Article
A simulation study with finite element model on the unequal loss of peripheral vision caused by acceleration
Next Article
A plane-based hand-eye calibration method for surgical robots

Journal of Biomedical Engineering

Influence of Fe₂O₃ nanoparticles on the rhological properties of simulated asthma airway mucus

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content

Journal of Biomedical Engineering

Influence of Fe2O3 nanoparticles on the rhological properties of simulated asthma airway mucus

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content

Influence of Fe₂O₃ nanoparticles on the rhological properties of simulated asthma airway mucus