Magnetic nanoparticles for specifically capturing endothelial progenitor cells and evaluation of its cellular compatibility_Journal of Biomedical Engineering

Authors：

FAN Yi ^1,2 , DU Zeyu ^1,2 ,  HUANG Nan ²

1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, P.R.China;
2. Key Laboratory of Advanced Technologies of Materials (KLATM), Ministry of Education, Southwest Jiaotong University, Chengdu 610031, P.R.China;

Corresponding author：

HUANG Nan, Email: huangnan1956@163.com

Keywords：

CD34 antibody; ferroferric oxide; endothelial progenitor cells; directional guide

DOI：

10.7507/1001-5515.201611060

Video：

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Immobilization of CD34 antibody on ferroferric oxide magnetic nanoparticles was achieved by the traditional carboxyl-amine conjugation reaction. Fourier transform infrared spectroscopy (FT-IR), nanoparticle size analysis (dynamic light scattering and transmission electron microscope), and other testing methods were used to detect the surface modified magnetic nanoparticles. The endothelial progenitor cells (EPCs) were cultured with the surface modified magnetic nanoparticles to evaluate cell compatibility and the combination effect of nanoparticles on EPCs in a short period of time. Directional guide of the surface modified magnetic nanoparticles to EPCs was evaluated under applied magnetic field and simulated dynamic flow condition. The results showed that the magnetic nanoparticles were successfully modified with CD34 antibody, which had good cell compatibility within a certain range of the nanoparticle concentrations. The surface modified nanoparticles can combine with EPCs effectively in a short time, and those nanoparticles combined EPCs can be directional guided under the magnetic field in the dynamic flow environment.

Citation： FAN Yi, DU Zeyu, HUANG Nan. Magnetic nanoparticles for specifically capturing endothelial progenitor cells and evaluation of its cellular compatibility. Journal of Biomedical Engineering, 2018, 35(6): 900-904, 913. doi: 10.7507/1001-5515.201611060 Copy

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1. Wu Liheng, Mendoza-Garcia A, Li Qing, et al. Organic phase syntheses of magnetic nanoparticles and their applications. Chem Rev, 2016, 116(18, SI): 10473-10512.
2. Kyrtatos P G, Lehtolainen P, Junemann-Ramirez M, et al. Magnetic tagging increases delivery of circulating progenitors in vascular injury. JACC Cardiovasc Interv, 2009, 2(8): 794-802.
3. Cheng Ke, Li Taosheng, Malliaras K, et al. Magnetic targeting enhances engraftment and functional benefit of iron-labeled cardiosphere-derived cells in myocardial infarction. Circ Res, 2010, 106(10): 1570-1581.
4. Vandergriff A C, Hensley T M, Henry E T, et al. Magnetic targeting of cardiosphere-derived stem cells with ferumoxytol nanoparticles for treating rats with myocardial infarction. Biomaterials, 2014, 35(30): 8528-8539.
5. Adamo R F, Fishbein I, Zhang Kehan, et al. Magnetically enhanced cell delivery for accelerating recovery of the endothelium in injured arteries. J Control Release, 2016, 222: 169-175.
6. Landázuri N, Tong Sheng, Suo Jin, et al. Magnetic targeting of human mesenchymal stem cells with internalized superparamagnetic iron oxide nanoparticles. Small, 2013, 9(23): 4017-4026.
7. Vosen S, Rieck S, Heidsieck A, et al. Vascular repair by circumferential cell therapy using magnetic nanoparticles and tailored magnets. ACS Nano, 2016, 10(1): 369-376.
8. Sun C, Lee J S, Zhang Miqin. Magnetic nanoparticles in MR imaging and drug delivery. Adv Drug Deliv Rev, 2008, 60(11): 1252-1265.
9. Ito A, Shinkai M, Honda H, et al. Medical application of functionalized magnetic nanoparticles. J Biosci Bioeng, 2005, 100(1): 1-11.

Journal of Biomedical Engineering

Magnetic nanoparticles for specifically capturing endothelial progenitor cells and evaluation of its cellular compatibility

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