Research advance of lateral flow assay labels_Journal of Biomedical Engineering

Authors：

JING Jing ,  WANG Zhizeng

Joint National Laboratory for Antibody Drug Engineering, Key Lab of Cellular and Molecular Immunology, School of Basic Medical Sciences, Henan University, Kaifeng, Henan 475004, P.R.China;

Corresponding author：

Keywords：

lateral flow assay; labels; colloidal gold; nanozyme

DOI：

10.7507/1001-5515.201703005

Video：

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Lateral flow assay is widely used in the point-of-care testing on-site and in-home testing with the advantage of being simple, rapid, sensitive and cost-effective. Proper labels are the key factors in lateral flow assay. Traditional labels include colloidal gold, selenium nanoparticle, and carbon nanoparticle, among which the colloidal gold is most commonly used. Lateral flow assay has been improved as a result of the discovery of new labels, such as quantum dots and nanozyme recently. Meanwhile, transformation of qualitative detection to quantitative detection is gradually realized. This article aims at introducing the most often used and the latest lateral flow assay labels, providing a basis theoretical investigation on screening proper labels for lateral flow assay researchers.

Citation： JING Jing, WANG Zhizeng. Research advance of lateral flow assay labels. Journal of Biomedical Engineering, 2018, 35(4): 661-664. doi: 10.7507/1001-5515.201703005 Copy

1.	Wang Zhizeng, Zhi Dejuan, Zhao Yang, et al. Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed. Int J Nanomedicine, 2014, 9(1): 1699-1707.
2.	Song Liuwei, Wang Yingbin, Fang Linlin, et al. Rapid fluorescent lateral-flow immunoassay for hepatitis B virus genotyping. Anal Chem, 2015, 87(10): 5173-5180.
3.	Teerinen T, Lappalainen T, Erho T. A paper-based lateral flow assay for morphine. Anal Bioanal Chem, 2014, 406(24): 5955-5965.
4.	Liu Liqiang, Xing Changrui, Yan Huijuan, et al. Development of an ELISA and immunochromatographic strip for highly sensitive detection of microcystin-LR. Sensors, 2014, 14(8): 14672-14685.
5.	Hu Jie, Wang Shuqi, Wang Lin, et al. Advances in paper-based point-of-care diagnostics. Biosens Bioelectron, 2014, 54(2): 585-597.
6.	Hagström A E V, Garvey G, Paterson A S, et al. Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay. PLoS One, 2015, 10(5): e0126571.
7.	Shan Shan, Lai Weihua, Xiong Yonghua, et al. Novel strategies to enhance lateral flow immunoassay sensitivity for detecting foodborne pathogens. J Agric Food Chem, 2015, 63(3): 745-753.
8.	Chandler J, Gurmin T, Robinson N. The place of gold in rapid tests. IVD Technol, 2000, 6(3): 37-49.
9.	Meng Kai, Sun Wenjing, Zhao Peng, et al. Development of colloidal gold-based immunochromatographic assay for rapid detection of Mycoplasma suis in porcine plasma. Biosens Bioelectron, 2014, 55: 396-399.
10.	Li Shuqun, Song Juan, Yang Hong, et al. An immunochromatographic assay for rapid and direct detection of 3-amino-5-morpholino-2-oxazolidone (AMOZ) in meat and feed samples. J Sci Food Agric, 2014, 94(4): 760-767.
11.	Hou Wanwan, Wang Shaohui, Wang Xiaolan, et al. Development of colloidal gold immunochromatographic strips for detection of riemerella anatipestifer. PLoS One, 2015, 10(3): e0122952.
12.	王志增. 三聚氰胺胶体硒快速检测试纸条的研制. 兰州: 兰州大学, 2014.
13.	Posthuma-Trumpie G A, Wichers J H, Koets M, et al. Amorphous carbon nanoparticles: a versatile label for rapid diagnostic (immuno)assays. Anal Bioanal Chem, 2012, 402(2): 593-600.
14.	Huang Xiaolin, Aguilar Z P, Xu Hengyi, et al. Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: A review. Biosens Bioelectron, 2016, 75: 166-180.
15.	Cui Y L, Hu D D, Fang Y, et al. Preparation and mechanism of Fe₃O₄/Au core/shell super-paramagnetic microspheres. Science in China Series B-chemistry, 2001, 44(4): 404-410.
16.	郭博阳, 马乐, 张梦丹, 等. 利用金磁微粒免疫化学发光技术检测高敏 C 反应蛋白. 细胞与分子免疫学杂志, 2015, 31(11): 1468-1472.
17.	Hui Wenli, Zhang Sinong, Zhang Chao, et al. A novel lateral flow assay based on GoldMag nanoparticles and its clinical applications for genotyping of MTHFR C677T polymorphisms. Nanoscale, 2016, 8(6): 3579-3587.
18.	韩文敏. 免疫检测荧光微球的制备和性能研究. 武汉: 华中科技大学, 2008.
19.	Cheng Song, Yang Yajie, Ni Xiaoqin, et al. Fluorescent microspheres lateral flow assay for sensitive detection of the milk allergen casein. Food Agric Immunol, 2017, 28(6): 1017-1028.
20.	Liu Daofeng, Huang Yanmei, Chen Minghui, et al. Rapid detection method for aflatoxin B-1 in soybean sauce based on fluorescent microspheres probe. Food Control, 2015, 50: 659-662.
21.	Xie Quanyuan, Wu Yanhua, Xiong Qirong, et al. Advantages of fluorescent microspheres compared with colloidal gold as a label in immunochromatographic lateral flow assays. Biosens Bioelectron, 2014, 54(12): 262-265.
22.	杨久敏. 基于纳米量子点标记肿瘤标志物检测系统研究. 天津: 天津大学, 2012.
23.	Chen Zhenhua, Liang Rongliang, Guo Xinxin, et al. Simultaneous quantitation of cytokeratin-19 fragment and carcinoembryonic antigen in human serum via quantum dot-doped nanoparticles. Biosens Bioelectron, 2017, 91: 60-65.
24.	Quesada-González D, Merkoçi A. Nanoparticle-based lateral flow biosensors. Biosens Bioelectron, 2015, 73(2): 47-63.
25.	Foubert A, Beloglazova N V, De Saeger S. Comparative study of colloidal gold and quantum dots as labels for multiplex screening tests for multi-mycotoxin detection. Anal Chim Acta, 2017, 955: 48-57.
26.	Bruno J G. Application of DNA aptamers and quantum dots to lateral flow test strips for detection of foodborne pathogens with improved sensitivity versus colloidal gold. Pathogens, 2014, 3(2): 341-355.
27.	赵瑾. 布鲁氏抗原快速检测 UTP 免疫层析试纸条的研制. 北京: 军事医学科学院疾病预防控制所, 2009.
28.	Bobosha K, Tjon Kon Fat E M, van den Eeden S J, et al. Field-evaluation of a new lateral flow assay for detection of cellular and humoral immunity against Mycobacterium leprae. PLoS Negl Trop Dis, 2014, 8(5): e2845.
29.	Das G K, Stark D T, Kennedy I M. Potential toxicity of up-converting nanoparticles encapsulated with a bilayer formed by ligand attraction. Langmuir, 2014, 30(27): 8167-8176.
30.	Zhang Pingping, Liu Xiao, Wang Chengbin, et al. Evaluation of up-converting phosphor technology-based lateral flow strips for rapid detection of Bacillus anthracis spore, Brucella spp., and Yersinia pestis. PLoS One, 2014, 9(8): e105305.
31.	Corstjens P L, de Dood C J, Priest J W, et al. Feasibility of a lateral flow test for neurocysticercosis using novel up-converting nanomaterials and a lightweight strip analyzer. PLoS Negl Trop Dis, 2014, 8(7): e2944.
32.	Chen Yiping, Xianyu Y, Sun Jiashu, et al. One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads. Nanoscale, 2016, 8(2): 1100-1107.
33.	Shukla S, Leem H, Kim M. Development of a liposome-based immunochromatographic strip assay for the detection of Salmonella. Anal Bioanal Chem, 2011, 401(8): 2581-2590.
34.	Duan Demin, Fan Kelong, Zhang Dexi, et al. Nanozyme-strip for rapid local diagnosis of Ebola. Biosens Bioelectron, 2015, 74: 134-141.
35.	Barnett J M, Wraith P, Kiely J, et al. An inexpensive, fast and sensitive quantitative lateral flow magneto-immunoassay for total prostate specific antigen. Biosensors, 2014, 4(3): 204-220.
36.	Wang Dianbing, Tian Bo, Zhang Zhiping, et al. Detection of Bacillus anthracis spores by super-paramagnetic lateral-flow immunoassays based on "Road Closure". Biosens Bioelectron, 2015, 67: 608-614.
37.	Gao Lizeng, Yan Xiyun. Nanozymes: an emerging field bridging nanotechnology and biology. Science China. Life sciences, 2016, 59(4): 400-402.

1. Wang Zhizeng, Zhi Dejuan, Zhao Yang, et al. Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed. Int J Nanomedicine, 2014, 9(1): 1699-1707.
2. Song Liuwei, Wang Yingbin, Fang Linlin, et al. Rapid fluorescent lateral-flow immunoassay for hepatitis B virus genotyping. Anal Chem, 2015, 87(10): 5173-5180.
3. Teerinen T, Lappalainen T, Erho T. A paper-based lateral flow assay for morphine. Anal Bioanal Chem, 2014, 406(24): 5955-5965.
4. Liu Liqiang, Xing Changrui, Yan Huijuan, et al. Development of an ELISA and immunochromatographic strip for highly sensitive detection of microcystin-LR. Sensors, 2014, 14(8): 14672-14685.
5. Hu Jie, Wang Shuqi, Wang Lin, et al. Advances in paper-based point-of-care diagnostics. Biosens Bioelectron, 2014, 54(2): 585-597.
6. Hagström A E V, Garvey G, Paterson A S, et al. Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay. PLoS One, 2015, 10(5): e0126571.
7. Shan Shan, Lai Weihua, Xiong Yonghua, et al. Novel strategies to enhance lateral flow immunoassay sensitivity for detecting foodborne pathogens. J Agric Food Chem, 2015, 63(3): 745-753.
8. Chandler J, Gurmin T, Robinson N. The place of gold in rapid tests. IVD Technol, 2000, 6(3): 37-49.
9. Meng Kai, Sun Wenjing, Zhao Peng, et al. Development of colloidal gold-based immunochromatographic assay for rapid detection of Mycoplasma suis in porcine plasma. Biosens Bioelectron, 2014, 55: 396-399.
10. Li Shuqun, Song Juan, Yang Hong, et al. An immunochromatographic assay for rapid and direct detection of 3-amino-5-morpholino-2-oxazolidone (AMOZ) in meat and feed samples. J Sci Food Agric, 2014, 94(4): 760-767.
11. Hou Wanwan, Wang Shaohui, Wang Xiaolan, et al. Development of colloidal gold immunochromatographic strips for detection of riemerella anatipestifer. PLoS One, 2015, 10(3): e0122952.
12. 王志增. 三聚氰胺胶体硒快速检测试纸条的研制. 兰州: 兰州大学, 2014.
13. Posthuma-Trumpie G A, Wichers J H, Koets M, et al. Amorphous carbon nanoparticles: a versatile label for rapid diagnostic (immuno)assays. Anal Bioanal Chem, 2012, 402(2): 593-600.
14. Huang Xiaolin, Aguilar Z P, Xu Hengyi, et al. Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: A review. Biosens Bioelectron, 2016, 75: 166-180.
15. Cui Y L, Hu D D, Fang Y, et al. Preparation and mechanism of Fe₃O₄/Au core/shell super-paramagnetic microspheres. Science in China Series B-chemistry, 2001, 44(4): 404-410.
16. 郭博阳, 马乐, 张梦丹, 等. 利用金磁微粒免疫化学发光技术检测高敏 C 反应蛋白. 细胞与分子免疫学杂志, 2015, 31(11): 1468-1472.
17. Hui Wenli, Zhang Sinong, Zhang Chao, et al. A novel lateral flow assay based on GoldMag nanoparticles and its clinical applications for genotyping of MTHFR C677T polymorphisms. Nanoscale, 2016, 8(6): 3579-3587.
18. 韩文敏. 免疫检测荧光微球的制备和性能研究. 武汉: 华中科技大学, 2008.
19. Cheng Song, Yang Yajie, Ni Xiaoqin, et al. Fluorescent microspheres lateral flow assay for sensitive detection of the milk allergen casein. Food Agric Immunol, 2017, 28(6): 1017-1028.
20. Liu Daofeng, Huang Yanmei, Chen Minghui, et al. Rapid detection method for aflatoxin B-1 in soybean sauce based on fluorescent microspheres probe. Food Control, 2015, 50: 659-662.
21. Xie Quanyuan, Wu Yanhua, Xiong Qirong, et al. Advantages of fluorescent microspheres compared with colloidal gold as a label in immunochromatographic lateral flow assays. Biosens Bioelectron, 2014, 54(12): 262-265.
22. 杨久敏. 基于纳米量子点标记肿瘤标志物检测系统研究. 天津: 天津大学, 2012.
23. Chen Zhenhua, Liang Rongliang, Guo Xinxin, et al. Simultaneous quantitation of cytokeratin-19 fragment and carcinoembryonic antigen in human serum via quantum dot-doped nanoparticles. Biosens Bioelectron, 2017, 91: 60-65.
24. Quesada-González D, Merkoçi A. Nanoparticle-based lateral flow biosensors. Biosens Bioelectron, 2015, 73(2): 47-63.
25. Foubert A, Beloglazova N V, De Saeger S. Comparative study of colloidal gold and quantum dots as labels for multiplex screening tests for multi-mycotoxin detection. Anal Chim Acta, 2017, 955: 48-57.
26. Bruno J G. Application of DNA aptamers and quantum dots to lateral flow test strips for detection of foodborne pathogens with improved sensitivity versus colloidal gold. Pathogens, 2014, 3(2): 341-355.
27. 赵瑾. 布鲁氏抗原快速检测 UTP 免疫层析试纸条的研制. 北京: 军事医学科学院疾病预防控制所, 2009.
28. Bobosha K, Tjon Kon Fat E M, van den Eeden S J, et al. Field-evaluation of a new lateral flow assay for detection of cellular and humoral immunity against Mycobacterium leprae. PLoS Negl Trop Dis, 2014, 8(5): e2845.
29. Das G K, Stark D T, Kennedy I M. Potential toxicity of up-converting nanoparticles encapsulated with a bilayer formed by ligand attraction. Langmuir, 2014, 30(27): 8167-8176.
30. Zhang Pingping, Liu Xiao, Wang Chengbin, et al. Evaluation of up-converting phosphor technology-based lateral flow strips for rapid detection of Bacillus anthracis spore, Brucella spp., and Yersinia pestis. PLoS One, 2014, 9(8): e105305.
31. Corstjens P L, de Dood C J, Priest J W, et al. Feasibility of a lateral flow test for neurocysticercosis using novel up-converting nanomaterials and a lightweight strip analyzer. PLoS Negl Trop Dis, 2014, 8(7): e2944.
32. Chen Yiping, Xianyu Y, Sun Jiashu, et al. One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads. Nanoscale, 2016, 8(2): 1100-1107.
33. Shukla S, Leem H, Kim M. Development of a liposome-based immunochromatographic strip assay for the detection of Salmonella. Anal Bioanal Chem, 2011, 401(8): 2581-2590.
34. Duan Demin, Fan Kelong, Zhang Dexi, et al. Nanozyme-strip for rapid local diagnosis of Ebola. Biosens Bioelectron, 2015, 74: 134-141.
35. Barnett J M, Wraith P, Kiely J, et al. An inexpensive, fast and sensitive quantitative lateral flow magneto-immunoassay for total prostate specific antigen. Biosensors, 2014, 4(3): 204-220.
36. Wang Dianbing, Tian Bo, Zhang Zhiping, et al. Detection of Bacillus anthracis spores by super-paramagnetic lateral-flow immunoassays based on "Road Closure". Biosens Bioelectron, 2015, 67: 608-614.
37. Gao Lizeng, Yan Xiyun. Nanozymes: an emerging field bridging nanotechnology and biology. Science China. Life sciences, 2016, 59(4): 400-402.

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