Progress in preparation and application of sodium alginate microspheres_Journal of Biomedical Engineering

Authors：

LIU Xuanyu ¹ , WANG Yuhui ¹ , LIANG Ziwei ^1,2 , LIAN Xiaojie ^1,2 ,  HUANG Di ^1,2 , HU Yinchun ^1,2 ,  WEI Yan ^1,2

1. Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, P. R. China;
2. Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, P. R. China;

Corresponding author：

HUANG Di, Email: huangjw2067@163.com

Keywords：

Sodium alginate microsphere; Bone repair; Hemostasis; Drug delivery; Biocompatibility

DOI：

10.7507/1001-5515.202211048

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Sodium alginate (SA) is a kind of natural polymer material extracted from kelp, which has excellent biocompatibility, non-toxicity, biodegradability and abundant storage capacity. The formation condition of sodium alginate gel is mild, effectively avoiding the inactivation of active substances. After a variety of preparation methods, sodium alginate microspheres are widely used in the fields of biomaterials and tissue engineering. This paper reviewed the common methods of preparing alginate microspheres, including extrusion, emulsification, electrostatic spraying, spray drying and coaxial airflow, and discussed their applications in biomedical fields such as bone repair, hemostasis and drug delivery.

Citation： LIU Xuanyu, WANG Yuhui, LIANG Ziwei, LIAN Xiaojie, HUANG Di, HU Yinchun, WEI Yan. Progress in preparation and application of sodium alginate microspheres. Journal of Biomedical Engineering, 2023, 40(4): 792-798. doi: 10.7507/1001-5515.202211048 Copy

1.	刘袖洞, 于炜婷, 王为, 等. 海藻酸钠和壳聚糖聚电解质微胶囊及其生物医学应用. 化学进展, 2008, 20(1): 14.
2.	Yang J, Yao J, Wang, S. Electromechanical response performance of a reinforced biomass gel artificial muscle based on natural polysaccharide of sodium alginate doped with an ionic liquid for micro-nano regulation. Carbohydr Polym, 2022, 275: 118717.
3.	Wang L, Zhang H J, Liu X, et al. A physically cross-linked sodium alginate–gelatin hydrogel with high mechanical strength. ACS Applied Polymer Materials, 2021, 3(6): 3197-3205.
4.	Liu J, Shang S, Jiang Z, et al. Facile fabrication of chemically modified sodium alginate fibers with enhanced mechanical performance. AATCC J Res, 2022(1): 9.
5.	周烨, 雷世婵, 罗勉, 等. 海藻酸钠微球制备方法进展. 生物化工, 2021, 7(1): 3.
6.	严丽华, 郭圣荣. 海藻酸钠微球的制备及其应用进展. 绿色科技, 2017(24): 4.
7.	Mørch Ýrr A, Donati I, Strand B L, et al. Effect of Ca²⁺, Ba^2+, and Sr²⁺ on alginate microbeads. Biomacromolecules, 2006, 7(5): 1471.
8.	Lee K Y, Mooney D J. Alginate: properties and biomedical applications. Prog Polym Sci, 2012, 37(1): 106-126.
9.	Tiraton T, Suwantong O, Chuysinuan P, et al. Biodegradable microneedle fabricated from sodium alginate-gelatin for transdermal delivery of clindamycin. Mater Today Commun, 2022, 32: 104158.
10.	Uyen N T T, Hamid Z A A, Tram N X T, et al. Fabrication of alginate microspheres for drug delivery: A review. Int J Biol Macromol, 2020, 153: 1035-1046.
11.	Brun-Graeppi A K, Richard C, Bessodes M, et al. Cell microcarriers and microcapsules of stimuli-responsive polymers. J Control Release, 2011, 149(3): 209-224.
12.	Guarino V, Altobelli R, Sala F D, et al. Alginate processing routes to fabricate bioinspired platforms for tissue engineering and drug delivery// Rehm B H A, Moradali M F. Alginates and their biomedical applications. Singapore: Springer Singapore, 2018: 101-120.
13.	Wang X, Zhu J, Shao T, et al. Production of highly monodisperse millimeter‐sized double‐emulsion droplets in a coaxial capillary device. Chem Eng Technol, 2019, 42(6): 1330-1340.
14.	Liao M, Wang C, Hong Y, et al. Industrial scale production of fibre batteries by a solution-extrusion method. Nat Nanotechnol, 2022, 17(4): 372-377.
15.	Ching S H, Bansal N, Bhandari B. Alginate gel particles-A review of production techniques and physical properties. Crit Rev Food Sci Nutr, 2017, 57(6): 1133-1152.
16.	Liew S N, Utra U, Alias A K, et al. Physical, morphological and antibacterial properties of lime essential oil nanoemulsions prepared via spontaneous emulsification method. LWT, 2020, 128(2): 109388.
17.	Chong D, Liu X, Ma H, et al. Advances in fabricating double-emulsion droplets and their biomedical applications. Microfluid Nanofluid, 2015, 19(5): 1071-1090.
18.	Ali R. Preparation and characterization of dexamethasone polymeric nanoparticle by membrane emulsification method. J Nanopart Res, 2020, 22(11): 314.
19.	Hayati I, Bailey A I, Tadros T F. Investigations into the mechanisms of electrohydrodynamic spraying of liquids. Pt.I. Effect of electric field and the environment on pendant drop and factors affecting the formation of stable jets and atomization. J Colloid Interface Sci, 1987, 117(1): 205–221.
20.	Hayati I, Bailey A, Tadros T F. Investigations into the mechanism of electrohydrodynamic spraying of liquids: II. Mechanism of stable jet formation and electrical forces acting on a liquid cone. J Colloid Interface Sci, 1986, 117(1): 222-230.
21.	Jaworek A. Micro- and nanoparticle production by electrospraying. Powder Technol, 2007, 176(1): 18-35.
22.	Xu M, Liu T, Qin M, et al. Bone-like hydroxyapatite anchored on alginate microspheres for bone regeneration. Carbohydr Polym, 2022, 287: 119330.
23.	Castrovilli M C, Tempesta E, Cartoni A, et al. Fabrication of a new, low-cost, and environment-friendly laccase-based biosensor by electrospray immobilization with unprecedented reuse and storage performances. ACS Sustain Chem Eng, 2022, 10(5): 1888-1898.
24.	Rehg T, Dorger C, Chau P C. Application of an atomizer in producing small alginate gel beads for cell immobilization. Biotechnol Lett, 1986, 8(2): 111-114.
25.	Salem D R. 1 - Electrospinning of nanofibers and the charge injection method// Brown P J, Stevens K. Nanofibers and nanotechnology in textiles. Sawston: Woodhead Publishing, 2007: 3-21.
26.	Workamp M, Alaie S, Dijksman J A. Coaxial air flow device for the production of millimeter-sized spherical hydrogel particles. Rev Sci Instrum, 2016, 87(12): 125113.
27.	Zhou J, Pei Z. Experimental study of the piezoelectric drop-on-demand drop formation in a coaxial airflow. Chem Eng Process, 2020, 147: 107778.
28.	Koch S, Schwinger C, Kressler J, et al. Alginate encapsulation of genetically engineered mammalian cells: Comparison of production devices, methods and microcapsule characteristics. J Microencapsul, 2003, 20(3): 303-316.
29.	储茂泉, 刘国杰. 喷雾干燥法制备载药微球时的形貌与粒度控制. 化工学报, 2004, 55(11): 1903-1907.
30.	Grenha A, Seijo B, Remunan-Lopez C. Microencapsulated chitosan nanoparticles for lung protein delivery. Eur J Pharm Sci, 2005, 25(4-5): 427-437.
31.	Yashaswini Devi G V, Prabhu A, Anil S, et al. Preparation and characterization of dexamethasone loaded sodium alginate-graphene oxide microspheres for bone tissue engineering. J Drug Deliv Sci Technol, 2021, 64: 102624.
32.	Thomas R G, Unnithan A R, Moon M J, et al. Electromagnetic manipulation enabled calcium alginate Janus microsphere for targeted delivery of mesenchymal stem cells. Int J Biol Macromol, 2018, 110: 465-471.
33.	Zhao D, Wang X, Cheng B, et al. Degradation-kinetics-controllable and tissue-regeneration-matchable photocross-linked alginate hydrogels for bone repair. ACS Appl Mater Interfaces, 2022, 14(19): 21886-21905.
34.	Miao F, Liu T, Zhang X, et al. Engineered bone tissues using biomineralized gelatin methacryloyl/sodium alginate hydrogels. J Biomater Sci Polym Ed, 2022, 33(2): 137-154.
35.	Huang X, Fu Q, Deng Y, et al. Surface roughness of silk fibroin/alginate microspheres for rapid hemostasis in vitro and in vivo. Carbohydr Polym, 2021, 253(1): 117256.
36.	Jin J, Ji Z, Xu M, et al. Microspheres of carboxymethyl chitosan, sodium alginate, and collagen as a hemostatic agent in vivo. ACS Biomater Sci Eng, 2018, 4(7): 2541-2551.
37.	Wang Y, Wang P, Ji H, et al. Analysis of safety and effectiveness of sodium alginate/poly (gamma-glutamic acid) microspheres for rapid hemostasis. ACS Appl Bio Mater, 2021, 4(8): 6539-6548.
38.	Xie M, Zeng Y, Wu H, et al. Multifunctional carboxymethyl chitosan/oxidized dextran/sodium alginate hydrogels as dressing for hemostasis and closure of infected wounds. Int J Biol Macromol, 2022, 219: 1337-1350.
39.	Chang S, Qin D, Yan R, et al. Temperature and pH dual responsive nanogels of modified sodium alginate and NIPAM for berberine loading and release. ACS Omega, 2021, 6(2): 1119-1128.
40.	Mao X, Li X, Zhang W, et al. Development of microspheres based on thiol-modified sodium alginate for intestinal-targeted drug delivery. ACS Appl Bio Mater, 2019, 2(12): 5810-5818.

1. 刘袖洞, 于炜婷, 王为, 等. 海藻酸钠和壳聚糖聚电解质微胶囊及其生物医学应用. 化学进展, 2008, 20(1): 14.
2. Yang J, Yao J, Wang, S. Electromechanical response performance of a reinforced biomass gel artificial muscle based on natural polysaccharide of sodium alginate doped with an ionic liquid for micro-nano regulation. Carbohydr Polym, 2022, 275: 118717.
3. Wang L, Zhang H J, Liu X, et al. A physically cross-linked sodium alginate–gelatin hydrogel with high mechanical strength. ACS Applied Polymer Materials, 2021, 3(6): 3197-3205.
4. Liu J, Shang S, Jiang Z, et al. Facile fabrication of chemically modified sodium alginate fibers with enhanced mechanical performance. AATCC J Res, 2022(1): 9.
5. 周烨, 雷世婵, 罗勉, 等. 海藻酸钠微球制备方法进展. 生物化工, 2021, 7(1): 3.
6. 严丽华, 郭圣荣. 海藻酸钠微球的制备及其应用进展. 绿色科技, 2017(24): 4.
7. Mørch Ýrr A, Donati I, Strand B L, et al. Effect of Ca²⁺, Ba^2+, and Sr²⁺ on alginate microbeads. Biomacromolecules, 2006, 7(5): 1471.
8. Lee K Y, Mooney D J. Alginate: properties and biomedical applications. Prog Polym Sci, 2012, 37(1): 106-126.
9. Tiraton T, Suwantong O, Chuysinuan P, et al. Biodegradable microneedle fabricated from sodium alginate-gelatin for transdermal delivery of clindamycin. Mater Today Commun, 2022, 32: 104158.
10. Uyen N T T, Hamid Z A A, Tram N X T, et al. Fabrication of alginate microspheres for drug delivery: A review. Int J Biol Macromol, 2020, 153: 1035-1046.
11. Brun-Graeppi A K, Richard C, Bessodes M, et al. Cell microcarriers and microcapsules of stimuli-responsive polymers. J Control Release, 2011, 149(3): 209-224.
12. Guarino V, Altobelli R, Sala F D, et al. Alginate processing routes to fabricate bioinspired platforms for tissue engineering and drug delivery// Rehm B H A, Moradali M F. Alginates and their biomedical applications. Singapore: Springer Singapore, 2018: 101-120.
13. Wang X, Zhu J, Shao T, et al. Production of highly monodisperse millimeter‐sized double‐emulsion droplets in a coaxial capillary device. Chem Eng Technol, 2019, 42(6): 1330-1340.
14. Liao M, Wang C, Hong Y, et al. Industrial scale production of fibre batteries by a solution-extrusion method. Nat Nanotechnol, 2022, 17(4): 372-377.
15. Ching S H, Bansal N, Bhandari B. Alginate gel particles-A review of production techniques and physical properties. Crit Rev Food Sci Nutr, 2017, 57(6): 1133-1152.
16. Liew S N, Utra U, Alias A K, et al. Physical, morphological and antibacterial properties of lime essential oil nanoemulsions prepared via spontaneous emulsification method. LWT, 2020, 128(2): 109388.
17. Chong D, Liu X, Ma H, et al. Advances in fabricating double-emulsion droplets and their biomedical applications. Microfluid Nanofluid, 2015, 19(5): 1071-1090.
18. Ali R. Preparation and characterization of dexamethasone polymeric nanoparticle by membrane emulsification method. J Nanopart Res, 2020, 22(11): 314.
19. Hayati I, Bailey A I, Tadros T F. Investigations into the mechanisms of electrohydrodynamic spraying of liquids. Pt.I. Effect of electric field and the environment on pendant drop and factors affecting the formation of stable jets and atomization. J Colloid Interface Sci, 1987, 117(1): 205–221.
20. Hayati I, Bailey A, Tadros T F. Investigations into the mechanism of electrohydrodynamic spraying of liquids: II. Mechanism of stable jet formation and electrical forces acting on a liquid cone. J Colloid Interface Sci, 1986, 117(1): 222-230.
21. Jaworek A. Micro- and nanoparticle production by electrospraying. Powder Technol, 2007, 176(1): 18-35.
22. Xu M, Liu T, Qin M, et al. Bone-like hydroxyapatite anchored on alginate microspheres for bone regeneration. Carbohydr Polym, 2022, 287: 119330.
23. Castrovilli M C, Tempesta E, Cartoni A, et al. Fabrication of a new, low-cost, and environment-friendly laccase-based biosensor by electrospray immobilization with unprecedented reuse and storage performances. ACS Sustain Chem Eng, 2022, 10(5): 1888-1898.
24. Rehg T, Dorger C, Chau P C. Application of an atomizer in producing small alginate gel beads for cell immobilization. Biotechnol Lett, 1986, 8(2): 111-114.
25. Salem D R. 1 - Electrospinning of nanofibers and the charge injection method// Brown P J, Stevens K. Nanofibers and nanotechnology in textiles. Sawston: Woodhead Publishing, 2007: 3-21.
26. Workamp M, Alaie S, Dijksman J A. Coaxial air flow device for the production of millimeter-sized spherical hydrogel particles. Rev Sci Instrum, 2016, 87(12): 125113.
27. Zhou J, Pei Z. Experimental study of the piezoelectric drop-on-demand drop formation in a coaxial airflow. Chem Eng Process, 2020, 147: 107778.
28. Koch S, Schwinger C, Kressler J, et al. Alginate encapsulation of genetically engineered mammalian cells: Comparison of production devices, methods and microcapsule characteristics. J Microencapsul, 2003, 20(3): 303-316.
29. 储茂泉, 刘国杰. 喷雾干燥法制备载药微球时的形貌与粒度控制. 化工学报, 2004, 55(11): 1903-1907.
30. Grenha A, Seijo B, Remunan-Lopez C. Microencapsulated chitosan nanoparticles for lung protein delivery. Eur J Pharm Sci, 2005, 25(4-5): 427-437.
31. Yashaswini Devi G V, Prabhu A, Anil S, et al. Preparation and characterization of dexamethasone loaded sodium alginate-graphene oxide microspheres for bone tissue engineering. J Drug Deliv Sci Technol, 2021, 64: 102624.
32. Thomas R G, Unnithan A R, Moon M J, et al. Electromagnetic manipulation enabled calcium alginate Janus microsphere for targeted delivery of mesenchymal stem cells. Int J Biol Macromol, 2018, 110: 465-471.
33. Zhao D, Wang X, Cheng B, et al. Degradation-kinetics-controllable and tissue-regeneration-matchable photocross-linked alginate hydrogels for bone repair. ACS Appl Mater Interfaces, 2022, 14(19): 21886-21905.
34. Miao F, Liu T, Zhang X, et al. Engineered bone tissues using biomineralized gelatin methacryloyl/sodium alginate hydrogels. J Biomater Sci Polym Ed, 2022, 33(2): 137-154.
35. Huang X, Fu Q, Deng Y, et al. Surface roughness of silk fibroin/alginate microspheres for rapid hemostasis in vitro and in vivo. Carbohydr Polym, 2021, 253(1): 117256.
36. Jin J, Ji Z, Xu M, et al. Microspheres of carboxymethyl chitosan, sodium alginate, and collagen as a hemostatic agent in vivo. ACS Biomater Sci Eng, 2018, 4(7): 2541-2551.
37. Wang Y, Wang P, Ji H, et al. Analysis of safety and effectiveness of sodium alginate/poly (gamma-glutamic acid) microspheres for rapid hemostasis. ACS Appl Bio Mater, 2021, 4(8): 6539-6548.
38. Xie M, Zeng Y, Wu H, et al. Multifunctional carboxymethyl chitosan/oxidized dextran/sodium alginate hydrogels as dressing for hemostasis and closure of infected wounds. Int J Biol Macromol, 2022, 219: 1337-1350.
39. Chang S, Qin D, Yan R, et al. Temperature and pH dual responsive nanogels of modified sodium alginate and NIPAM for berberine loading and release. ACS Omega, 2021, 6(2): 1119-1128.
40. Mao X, Li X, Zhang W, et al. Development of microspheres based on thiol-modified sodium alginate for intestinal-targeted drug delivery. ACS Appl Bio Mater, 2019, 2(12): 5810-5818.

Previous Article
Advances in digital twins technology of human skeletal muscle
Next Article
Research progress of coarse-grained molecular dynamics in drug carrier materials

Journal of Biomedical Engineering

Progress in preparation and application of sodium alginate microspheres

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content