Effects of CD14 on the Activity of Gastric Cancer Cell Nuclear Factor-κB and the Expression of Human β-Defensin-2_West China Medical Journal

Authors：

 LI Kang , DAN Zeng , WANG Jia , GANG Zu , ZE Yongge.

Department of Gastroenterology, People’s Hospital of Tibet Autonomous Region, Lhasa, Tibet 850000, P. R. China;

Corresponding author：

LI Kang, Email: likang820@yahoo.com.cn

Keywords：

CD14; 胃癌细胞; 核转录因子-κB; 人β防御素-2

DOI：

10.7507/1002-0179.20130107

Video：

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目的　研究CD14过表达对胃癌细胞核转录因子-κB（NF-κB）活性以及人β防御素-2（hBD-2）表达的影响，探讨CD14在胃癌发生发展中的作用。方法　体外培养CD14稳定转染的胃癌SGC-7901细胞系及空质粒转染的对照细胞，CD14蛋白受体胞壁酰二肽刺激细胞，凝胶迁移实验检测NF-κB的活性，蛋白质印迹法检测NF-κB蛋白的表达，同时分别用逆转录-聚合酶链反应以及蛋白质印迹法检测hBD-2 mRNA及蛋白的表达。结果　与对照组相比，CD14过表达的细胞中NF-κB的活性明显增强，蛋白表达量也大幅度增加，同时hBD-2的mRNA及蛋白的表达都有所提高。结论　胃癌细胞中CD14在介导NF-κB的激活以及hBD-2的表达中发挥重要作用。

Citation： LI Kang,DAN Zeng,WANG Jia,GANG Zu,ZE Yongge.. Effects of CD14 on the Activity of Gastric Cancer Cell Nuclear Factor-κB and the Expression of Human β-Defensin-2. West China Medical Journal, 2013, 28(3): 323-326. doi: 10.7507/1002-0179.20130107 Copy

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7.	Miyake K. Innate immune sensing of pathogens and danger signals by cell surface Toll-like receptors[J]. Semin Immunol, 2007, 19(1): 3-10.
8.	Li K, Dan Z, Hu X, et al. CD14 overexpression upregulates TNF-a-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells[J]. Mol Cell Biochem, 2013, 376(1-2): 137-143.
9.	Midorikawa K, Ouhara K, Komatsuzawa H, et al. Staphylococcus aureus susceptibility to innate antimicrobial peptides, beta-defensins and CAP18, expressed by human keratinocytes[J]. Infect Immum, 2003, 71(7): 3730-3739.
10.	Hiratsuka T, Mukae H, Iiboshi H, et al. Increased concentrations of human beta-defensins in plasma and bronchoalveolar lavage fluid of patients with diffuse panbrochiolitis[J]. Thorax, 2003, 58(5): 425-430.
11.	Tsutsumi-Ishii Y, Nagaoka I. NF-kappa B mediated transcriptional regulation of human beta- defensin-2 gene following lipopolysaccharide stimulation[J]. J Leukoc Biol, 2002, 71(1): 154-162.
12.	Tomita T, Nagase T, Ohga E, et al. Molecular mechanisms underlying human beta-defensin-2 gene expression in a human airway cell line(LC2/ad)[J]. Respirology, 2002, 7(4): 305-310.

1. 　Ferlay J, Shin HR, Bray F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008[J]. Int J Cancer, 2010, 127(12): 2893-2917.
2. 　Zhao D, Sun T, Zhang X, et al. Role of CD14 Promoter Polymorphisms in Helicobacter pylori Infection Related Gastric Carcinoma[J]. Clin Cancer Res, 2007, 13(8): 2362-2368.
3. 　Goyert SM, Ferrero E, Rettig WJ, et al. The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors[J]. Science, 1988, 239(4839): 497-500.
4. 　Zhang DE, Hetherington CJ, Tan S, et al. Sp1 is a critical factor for the monocytic specific expression of human CD14[J]. J Biol Chem,1994, 269(15): 11425-11434.
5. 　Netea MG, Kullberg BJ, van der Meer JW. Lipopolysaccharide induced production of tumour necrosis factor and interleukin-1 is differentially regulated at the receptor level:the role of CD14-dependent and CD14-independent pathways[J]. Immunology,1998, 94(3): 340-344.
6. 　Hold GL, Rabkin CS, Gammon MD, et al. CD14-159C/T and TLR9-1237T/C polymorphisms are not associated with gastric cancer risk in Caucasian populations[J]. Eur J Cancer Prev, 2009, 18(2): 117-119.
7. 　Miyake K. Innate immune sensing of pathogens and danger signals by cell surface Toll-like receptors[J]. Semin Immunol, 2007, 19(1): 3-10.
8. 　Li K, Dan Z, Hu X, et al. CD14 overexpression upregulates TNF-a-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells[J]. Mol Cell Biochem, 2013, 376(1-2): 137-143.
9. 　Midorikawa K, Ouhara K, Komatsuzawa H, et al. Staphylococcus aureus susceptibility to innate antimicrobial peptides, beta-defensins and CAP18, expressed by human keratinocytes[J]. Infect Immum, 2003, 71(7): 3730-3739.
10. 　Hiratsuka T, Mukae H, Iiboshi H, et al. Increased concentrations of human beta-defensins in plasma and bronchoalveolar lavage fluid of patients with diffuse panbrochiolitis[J]. Thorax, 2003, 58(5): 425-430.
11. 　Tsutsumi-Ishii Y, Nagaoka I. NF-kappa B mediated transcriptional regulation of human beta- defensin-2 gene following lipopolysaccharide stimulation[J]. J Leukoc Biol, 2002, 71(1): 154-162.
12. 　Tomita T, Nagase T, Ohga E, et al. Molecular mechanisms underlying human beta-defensin-2 gene expression in a human airway cell line(LC2/ad)[J]. Respirology, 2002, 7(4): 305-310.

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Effects of CD14 on the Activity of Gastric Cancer Cell Nuclear Factor-κB and the Expression of Human β-Defensin-2

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