The Study of Skp2 Expression in Human Testicular Germ Cell Tumors_West China Medical Journal

Authors：

FU Guangqing ¹ , HUANG Jin ² , LIANG Yong ¹ , HUANG Fengming ¹ , HAN Dengjun. ¹

1. Department of Urology, Zigong No. 4 People’s Hospital, Zigong, Sichuan 643000, P. R. China; 2. West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China;

Corresponding author：

Keywords：

S期激酶相关蛋白2; 泛素-蛋白酶体途径; 睾丸生殖细胞肿瘤; 免疫组织化学

DOI：

10.7507/1002-0179.20130318

Video：

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目的　通过检测人睾丸生殖细胞肿瘤中的Skp2蛋白质异常表达,探讨相关意义。方法　应用S-P免疫组织化学法检测睾丸生殖细胞肿瘤，正常睾丸组织和慢性睾丸炎组织中Skp2的表达。结果　睾丸生殖细胞肿瘤中Skp2阳性表达率为74.5%，正常睾丸组织中Skp2阳性表达率为20.0%，在慢性睾丸炎组织中Skp2阳性表达率为40.0%，在3种不同睾丸组织中表达差异有统计学意义（P＜0.05）；Skp2表达与不同组织学类型的睾丸生殖细胞肿瘤无相关性（P＞0.05）；随着临床分期的增高，睾丸生殖细胞肿瘤中的Skp2表达增多，差异无统计学意义（P＞0.05）。结论　在人睾丸生殖细胞肿瘤中的Skp2高表达，提示细胞周期的异常调控在睾丸生殖细胞肿瘤的发生、分化中起着重要的作用。

Citation： FU Guangqing,HUANG Jin,LIANG Yong,HUANG Fengming,HAN Dengjun.. The Study of Skp2 Expression in Human Testicular Germ Cell Tumors. West China Medical Journal, 2013, 28(7): 1029-1032. doi: 10.7507/1002-0179.20130318 Copy

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2.	Remmele W, Stegner HE. Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue[J]. Pathologe, 1987, 8(3): 138-140.
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4.	罗四维, 彭先兵, 张群, 等. Skp2结构与肿瘤关系研究进展[J]. 中华实用诊断与治疗杂志, 2010, 24(2): 110-112.
5.	Latres E, Chiarle R, Schulman BA, et al. Role of the F-box protein Skp2 in lymphomagenesis[J]. Proc Natl Acad Sci USA, 2001, 98(5): 2515-2520.
6.	赵君, 杨春鹿, 赵苏英. 肺癌中细胞S期激酶相关蛋白2的表达及其对预后的影响[J]. 中华肿瘤杂志, 2007, 29(4): 289-292.
7.	Masuda TA, Inoue H, Sonoda H, et al. Clinical and biological significance of S-phase kinase-associated protein 2 (Skp2) gene expression in gastric carcinoma modulation of malignant phenotype by Skp20 over expression possibly via p27 proteolysis[J]. Cancer Res, 2002, 62(13): 3819-3825.
8.	Yang G, Ayala G, De Marzo A, et al. Elevated Skp2 protein expression in human prostate Cancer: association with loss of the cyclin-dependent kinase inhibitor p27 and PTEN and with reduced recurrence-free survival[J]. Clin Cancer Res, 2002, 8(11): 3419-3426.
9.	蒋国良. Skp2蛋白在卵巢上皮性肿瘤中的表达及意义[J]. 苏州医学, 2010, 33(2): 87-90.
10.	隋战胜, 李香波, 李香玉, 等. 应用组织芯片技术研究Skp2、p27在膀胱移行细胞癌中的表达及意义[J]. 临床外科杂志, 2011, 19(7): 483-485.
11.	Chiappetta G, De Marco C, Quintiero A, et al. Overexpression of the S-phase kinase-associated protein 2 in thyroid cancer[J]. Endocr Relat Cancer, 2007, 14(2): 405-420.
12.	Koga H, Harada M, Ohtsubo M, et al. Troglitazone induces p27Kip1-associated cell-cycle arrest through down-regulating Skp2 in human hepatoma cells[J]. Hepatology, 2003, 37(5): 1086-1096.
13.	Lin R, Wang TT, Miller WH Jr, et al. Inhibition of F-Box protein p45(SKP2) expression and stabilization of cyclin-dependent kinase inhibitor p27(KIP1) in vitamin D analog-treated cancer cells[J]. Endocrinology, 2003, 144(3): 749-753.
14.	Ishii T, Matsuse T, Masuda M, et al. The effects of S-phase kinase-associated protein 2 (SKP2) on cell cycle status, viability, and chemoresistance in A549 lung adenocarcinoma cells[J]. Exp Lung Res, 2004, 30(8): 687-703.
15.	吕会增, 朱晓峰. Skp2,p27蛋白表达与胃癌的临床关系[J]. 中国医师杂志, 2005, 7(6): 751-753.
16.	Dowen SE, Scott A, Mukherjee G, et al. Overexpression of Skp2 in carcinoma of the cervix does not correlate inversely with p27 expression[J]. Int J Cancer, 2003, 105(3): 326-330.
17.	Sui L, Dong Y, Watanabe Y, et al. Clinical significance of Skp2 expression,alone an d combined with Jabl and p27 inepithelial ovarian tumors[J]. Oncol Rep, 2006, 15(4): 765-771.

1. 　吴阶平. 吴阶平泌尿外科学[M]. 济南: 山东科学技术出版社, 2003: 993.
2. 　Remmele W, Stegner HE. Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue[J]. Pathologe, 1987, 8(3): 138-140.
3. 　何洪智, 赵晓航, 张立勇, 等. 蛋白质泛素化降解途径与肿瘤发生的关系[J]. 世界华人消化杂志, 2002, 10(12): 1365-1368.
4. 　罗四维, 彭先兵, 张群, 等. Skp2结构与肿瘤关系研究进展[J]. 中华实用诊断与治疗杂志, 2010, 24(2): 110-112.
5. 　Latres E, Chiarle R, Schulman BA, et al. Role of the F-box protein Skp2 in lymphomagenesis[J]. Proc Natl Acad Sci USA, 2001, 98(5): 2515-2520.
6. 　赵君, 杨春鹿, 赵苏英. 肺癌中细胞S期激酶相关蛋白2的表达及其对预后的影响[J]. 中华肿瘤杂志, 2007, 29(4): 289-292.
7. 　Masuda TA, Inoue H, Sonoda H, et al. Clinical and biological significance of S-phase kinase-associated protein 2 (Skp2) gene expression in gastric carcinoma modulation of malignant phenotype by Skp20 over expression possibly via p27 proteolysis[J]. Cancer Res, 2002, 62(13): 3819-3825.
8. 　Yang G, Ayala G, De Marzo A, et al. Elevated Skp2 protein expression in human prostate Cancer: association with loss of the cyclin-dependent kinase inhibitor p27 and PTEN and with reduced recurrence-free survival[J]. Clin Cancer Res, 2002, 8(11): 3419-3426.
9. 　蒋国良. Skp2蛋白在卵巢上皮性肿瘤中的表达及意义[J]. 苏州医学, 2010, 33(2): 87-90.
10. 　隋战胜, 李香波, 李香玉, 等. 应用组织芯片技术研究Skp2、p27在膀胱移行细胞癌中的表达及意义[J]. 临床外科杂志, 2011, 19(7): 483-485.
11. 　Chiappetta G, De Marco C, Quintiero A, et al. Overexpression of the S-phase kinase-associated protein 2 in thyroid cancer[J]. Endocr Relat Cancer, 2007, 14(2): 405-420.
12. 　Koga H, Harada M, Ohtsubo M, et al. Troglitazone induces p27Kip1-associated cell-cycle arrest through down-regulating Skp2 in human hepatoma cells[J]. Hepatology, 2003, 37(5): 1086-1096.
13. 　Lin R, Wang TT, Miller WH Jr, et al. Inhibition of F-Box protein p45(SKP2) expression and stabilization of cyclin-dependent kinase inhibitor p27(KIP1) in vitamin D analog-treated cancer cells[J]. Endocrinology, 2003, 144(3): 749-753.
14. 　Ishii T, Matsuse T, Masuda M, et al. The effects of S-phase kinase-associated protein 2 (SKP2) on cell cycle status, viability, and chemoresistance in A549 lung adenocarcinoma cells[J]. Exp Lung Res, 2004, 30(8): 687-703.
15. 　吕会增, 朱晓峰. Skp2,p27蛋白表达与胃癌的临床关系[J]. 中国医师杂志, 2005, 7(6): 751-753.
16. 　Dowen SE, Scott A, Mukherjee G, et al. Overexpression of Skp2 in carcinoma of the cervix does not correlate inversely with p27 expression[J]. Int J Cancer, 2003, 105(3): 326-330.
17. 　Sui L, Dong Y, Watanabe Y, et al. Clinical significance of Skp2 expression,alone an d combined with Jabl and p27 inepithelial ovarian tumors[J]. Oncol Rep, 2006, 15(4): 765-771.

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