The Effect of Traditional Tibetan Tonic Prescription Basangmusuyou Pills on Proliferation Ability of Bone Marrow Cells in Mice Injured by Radiation and Chemistry_West China Medical Journal

Authors：

HUANG Xiaoqin ¹ , Jiangyangzeren ² , LAI Li ² , GUAN Yan ²

1. National Medical School; 2. Preclinical School, Chengdu Traditional Chinese Medicine University, Chengdu, Sichuan 611137, P. R. China;

Corresponding author：

Keywords：

巴桑母酥油; 放射治疗; 化学疗法; 骨髓细胞; 造血干细胞; 造血祖细胞

DOI：

10.7507/1002-0179.20130377

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的　观察藏医滋补方“巴桑母酥油丸”对放射线-化学复合损伤小鼠骨髓不同细胞群增殖能力的影响，探讨其促进放射线-化学复合损伤机体外周血象恢复的机制。方法　采用造血祖细胞集落分析方法、流式细胞术，检测灌胃巴桑母酥油丸后放射线-化学复合损伤小鼠骨髓细胞中早期红系祖细胞（CFU-E）、晚期造血祖细胞（BFU-E）、粒-巨噬系祖细胞（CFU-GM）、巨核系祖细胞（CFU-Meg）集落产率、骨髓细胞增殖周期各时相细胞比例、造血干细胞抗原-1（Sca-1）免疫表型阳性细胞数变化情况。结果　巴桑母酥油丸组骨髓细胞S+G2/M期细胞比例高于生理盐水组（P＜0.05）和自然恢复的空白组（P＜0.01）；CFU-E、BFU-E、CFU-GM集落产率高于生理盐水组和空白组（P＜0.05）；CFU-Meg集落产率、Sca-1+细胞数在各组间差异无统计学意义（P＞0.05）。结论　巴桑母酥油丸对放射线-化学复合损伤小鼠骨髓细胞具有促进增殖的作用，这可能是其促进放射线-化学复合损伤机体外周血象恢复的途径，但对不同阶段、不同系别的造血细胞其促进作用不同。

Citation： HUANG Xiaoqin,Jiangyangzeren,LAI Li,GUAN Yan. The Effect of Traditional Tibetan Tonic Prescription Basangmusuyou Pills on Proliferation Ability of Bone Marrow Cells in Mice Injured by Radiation and Chemistry. West China Medical Journal, 2013, 28(8): 1207-1210. doi: 10.7507/1002-0179.20130377 Copy

1.	宇妥·云丹衮波. 医学四续[M]. 李多美, 译. 西安: 西安出版社, 2000: 631.
2.	曾科,牛挺. 电离辐射的危害及放射线检查的健康风险[J].华西医学, 2011, 26(12): 1814-1817.
3.	第司·桑吉嘉措. 蓝琉璃[M]. 毛继祖, 卡洛, 毛韶玲, 译. 上海: 上海科学技术出版社, 2012: 617.
4.	黄晓芹, 赖先荣, 降央泽仁, 等. 藏药巴桑母酥油丸对放、化复合损伤“血虚证”小鼠造血功能干预初探[J]. 中药与临床, 2010, 1(2): 38-39.
5.	黄晓芹, 降央泽仁, 祝彼得. 放、化复合损伤至血虚证小鼠肾脏EPO、脾脏EPO-R、骨髓GM-CSF mRNA表达改变[J]. 成都中医药大学学报, 2009, 32(3): 56-60.
6.	Kaimakis P, Crisan M, Dzierzak E. The biochemistry of hematopoietic stem cell development[J]. Biochim Biophys Acta, 2013, 1830(2): 2395-2403.
7.	Van Zant G, Liang Y. Concise review: hematopoietic stem cell aging, life span, and transplantation[J]. Stem Cells Transl Med, 2012, 1(9): 651-657.
8.	Walasek MA, van Os R, de Haan G. Hematopoietic stem cell expansion: challenges and opportunities[J]. Ann N Y Acad Sci, 2012, 1266: 138-150.
9.	Takizawa H, Schanz U, Manz MG. Ex vivo expansion of hematopoietic stem cells: mission accomplished?[J]. Swiss Med Wkly, 2011, 141: 13316.
10.	Holmes C, Stanford WL. Concise review: stem cell antigen-1: expression, function, and enigma[J]. Stem Cells, 2007, 25(6): 1339-1347.
11.	Zhang CC, Lodish HF. Murine hematopoietic stem cells change their surface phenotype during ex vivo expansion[J]. Blood, 2005, 105(11): 4314-1420.
12.	Spangrude GJ, Brooks DM. Mouse strain variability in the expression of the hematopoietic stem cell antigen Ly-6A/E by bone marrow cells[J]. Blood, 1993, 82(11): 3327-3332.
13.	Uchida N, Weissman IL. Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow[J]. J Exp Med, 1992, 175(1): 175-184.
14.	Ito CY, Li CY, Bernstein A, et al. Hematopoietic stem cell and progenitor defects in Sca-1/Ly-6A-null mice[J]. Blood, 2003, 101(2): 517-523.
15.	Bradfute SB, Graubert TA, Goodell MA. Roles of Sca-1 in hematopoietic stem/progenitor cell function[J]. Exp Hematol, 2005, 33(7): 836-843.
16.	Balasubramani SP, Venkatasubramanian P, Kukkupuni SK, et al. Plant-based Rasayana drugs from Ayurveda[J]. Chin J Intear Med, 2011, 17(2): 88-94.
17.	Vayalil PK, Kuttan G, Kuttan R. Protective effects of Rasayanas on cyclophosphamideand radiation-induced damage[J]. J Altern Complement Med, 2002, 8(6): 787-796.
18.	张忠诚. 印度阿育吠陀医学研究现状[J]. 国外医药·植物药分册, 2000, 15(1): 18-22.
19.	李秀丽, 叶峰, 俞腾飞. 余甘子的药理研究进展[J]. 时珍国医国药, 2006, 17(2): 266-267.
20.	王红玲, 熊顺军, 洪艳, 等. 黄精多糖对全身60Coγ射线照射小鼠外周血细胞数量及功能的影响[J]. 数理医药学杂志, 2000, 13(6): 493-494.
21.	刘萍. 黄精多糖对老龄大鼠老化相关酶的影响[J]. 华西医学, 2010, 25(7): 1259-1261.
22.	帝玛尔·丹增彭措. 晶珠本草[M]. 毛继祖, 林鹏程, 吉守祥, 等, 译. 上海: 上海科学技术出版社, 2012: 217.

1. 　宇妥·云丹衮波. 医学四续[M]. 李多美, 译. 西安: 西安出版社, 2000: 631.
2. 　曾科,牛挺. 电离辐射的危害及放射线检查的健康风险[J].华西医学, 2011, 26(12): 1814-1817.
3. 　第司·桑吉嘉措. 蓝琉璃[M]. 毛继祖, 卡洛, 毛韶玲, 译. 上海: 上海科学技术出版社, 2012: 617.
4. 　黄晓芹, 赖先荣, 降央泽仁, 等. 藏药巴桑母酥油丸对放、化复合损伤“血虚证”小鼠造血功能干预初探[J]. 中药与临床, 2010, 1(2): 38-39.
5. 　黄晓芹, 降央泽仁, 祝彼得. 放、化复合损伤至血虚证小鼠肾脏EPO、脾脏EPO-R、骨髓GM-CSF mRNA表达改变[J]. 成都中医药大学学报, 2009, 32(3): 56-60.
6. 　Kaimakis P, Crisan M, Dzierzak E. The biochemistry of hematopoietic stem cell development[J]. Biochim Biophys Acta, 2013, 1830(2): 2395-2403.
7. 　Van Zant G, Liang Y. Concise review: hematopoietic stem cell aging, life span, and transplantation[J]. Stem Cells Transl Med, 2012, 1(9): 651-657.
8. 　Walasek MA, van Os R, de Haan G. Hematopoietic stem cell expansion: challenges and opportunities[J]. Ann N Y Acad Sci, 2012, 1266: 138-150.
9. 　Takizawa H, Schanz U, Manz MG. Ex vivo expansion of hematopoietic stem cells: mission accomplished?[J]. Swiss Med Wkly, 2011, 141: 13316.
10. 　Holmes C, Stanford WL. Concise review: stem cell antigen-1: expression, function, and enigma[J]. Stem Cells, 2007, 25(6): 1339-1347.
11. 　Zhang CC, Lodish HF. Murine hematopoietic stem cells change their surface phenotype during ex vivo expansion[J]. Blood, 2005, 105(11): 4314-1420.
12. 　Spangrude GJ, Brooks DM. Mouse strain variability in the expression of the hematopoietic stem cell antigen Ly-6A/E by bone marrow cells[J]. Blood, 1993, 82(11): 3327-3332.
13. 　Uchida N, Weissman IL. Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow[J]. J Exp Med, 1992, 175(1): 175-184.
14. 　Ito CY, Li CY, Bernstein A, et al. Hematopoietic stem cell and progenitor defects in Sca-1/Ly-6A-null mice[J]. Blood, 2003, 101(2): 517-523.
15. 　Bradfute SB, Graubert TA, Goodell MA. Roles of Sca-1 in hematopoietic stem/progenitor cell function[J]. Exp Hematol, 2005, 33(7): 836-843.
16. 　Balasubramani SP, Venkatasubramanian P, Kukkupuni SK, et al. Plant-based Rasayana drugs from Ayurveda[J]. Chin J Intear Med, 2011, 17(2): 88-94.
17. 　Vayalil PK, Kuttan G, Kuttan R. Protective effects of Rasayanas on cyclophosphamideand radiation-induced damage[J]. J Altern Complement Med, 2002, 8(6): 787-796.
18. 　张忠诚. 印度阿育吠陀医学研究现状[J]. 国外医药·植物药分册, 2000, 15(1): 18-22.
19. 　李秀丽, 叶峰, 俞腾飞. 余甘子的药理研究进展[J]. 时珍国医国药, 2006, 17(2): 266-267.
20. 　王红玲, 熊顺军, 洪艳, 等. 黄精多糖对全身60Coγ射线照射小鼠外周血细胞数量及功能的影响[J]. 数理医药学杂志, 2000, 13(6): 493-494.
21. 　刘萍. 黄精多糖对老龄大鼠老化相关酶的影响[J]. 华西医学, 2010, 25(7): 1259-1261.
22. 　帝玛尔·丹增彭措. 晶珠本草[M]. 毛继祖, 林鹏程, 吉守祥, 等, 译. 上海: 上海科学技术出版社, 2012: 217.

Previous Article
Arthroscopically Assisted Suturing to Repair Bucket Handle Meniscal Tear
Next Article
Mechanism of Radioresistance Function of Water Etracts from Coix Lachrymal-jobi

West China Medical Journal

The Effect of Traditional Tibetan Tonic Prescription Basangmusuyou Pills on Proliferation Ability of Bone Marrow Cells in Mice Injured by Radiation and Chemistry

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content