Expression of CRABPⅡ, E-FABP and Ki67 in Breast Invasive Ductal Carcinoma and Their Correlation_West China Medical Journal

Authors：

MENG Hong ¹ , LIU Qian ¹ , WU Ning ¹ , JIANG Xuemei ² , HE Xiaoyan ¹ , DING Wuwu. ¹

1. Department of Pathology; 2. Department of Breast Surgery; People’s Hospital of Deyang city, Deyang, Sichuan 618000, P. R. China;

Corresponding author：

Keywords：

乳腺肿瘤; 浸润性导管癌; 细胞视黄酸结合蛋白Ⅱ; 表皮型脂肪酸结合蛋白; Ki-67抗原

DOI：

10.7507/1002-0179.20130444

Video：

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目的　研究细胞视黄酸结合蛋白（CRABP）Ⅱ、表皮型脂肪酸结合蛋白（E-FABP）和Ki-67在乳腺浸润性导管癌中的表达情况及三者的相关性。方法　采用免疫组织化学检测2001年1月－2007年12月手术切除的152例乳腺浸润性导管癌中CRABPⅡ、E-FABP和Ki-67的表达。结果　在浸润性导管癌中，CRABPⅡ在Ki-67阴性组的阳性率高于Ki-67阳性组（P＜0.05），相反地，E-FABP在Ki-67阳性组的阳性率高于Ki-67阴性组（P＜0.05）。CRABPⅡ和Ki-67表达呈负相关（rS=?0.432，P＜0.05）；E-FABP和Ki-67表达呈正相关（rS=0.842， P＜0.05）。E-FABP和Ki-67的表达具有协同性，E-FABP和Ki-67共同表达与肿瘤的转移有关（P＜0.05）。单因素生存分析显示，E-FABP的阳性表达患者、Ki-67的阳性表达患者以及E-FABP和Ki-67的共同阳性表达患者的预后差（P＜0.05）。多因素生存分析提示E-FABP的表达（RR=4.223，P=0.012）和TNM分期（RR=8.412，P=0.000）是影响浸润性导管癌患者预后的独立危险因素。结论　在乳腺浸润性导管癌中，CRABPⅡ和E-FABP与肿瘤细胞的增殖有关，CRABPⅡ抑制细胞增殖，E-FABP促进细胞增殖。E-FABP和Ki-67在浸润性导管癌的发生、发展中起协同作用，两者的阳性表达可能对评估肿瘤的转移和患者的预后有一定价值。

Citation： MENG Hong,LIU Qian,WU Ning,JIANG Xuemei,HE Xiaoyan,DING Wuwu.. Expression of CRABPⅡ, E-FABP and Ki67 in Breast Invasive Ductal Carcinoma and Their Correlation. West China Medical Journal, 2013, 28(9): 1415-1419. doi: 10.7507/1002-0179.20130444 Copy

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14.	Bouzubar N, Walker KJ, Griffiths K, et al. Ki-67 immunostaining in primary breast cancer: pathological and clinical associations[J]. Br J Cancer, 1989, 59(6): 943-947.
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1. 　Schug TT, Berry DC, Shaw NS, et al. Opposing effects of retinoic acid on cell growth result from alternate activation of two different nuclear receptors[J]. Cell, 2007, 129(4): 723-733.
2. 　Rochette-Egly C, Chambon P. F9 embryocarcinoma cells: a cell autonomous model to study the functional selectivity of RARs and RXRs in retinoid signaling[J]. Histol Histopathol, 2001, 16(3): 909-922.
3. 　Donato LJ, Suh JH, Noy N. Suppression of mammary carcinoma cell growth by retinoic acid: the cell cycle control gene Btg2 is a direct target for retinoic acid receptor signaling[J]. Cancer Res, 2007, 67(2): 609-615.
4. 　Donato LJ, Noy N. Suppression of mammary carcinoma growth by retinoic acid: proapoptotic genes are targets for retinoic acid receptor and cellular retinoic acid-binding proteinⅡ signaling[J]. Cancer Res, 2005, 65(18): 8193-8199.
5. 　Di-Poi N, Tan NS, Michalik L, et al. Antiapoptotic role of PPAR beta in keratinocytes via transcriptional control of the akt1 signaling pathway[J]. Mol Cell, 2002, 10(4): 721-733.
6. 　Vo HP, Crowe DL. Transcriptional regulation of retinoic acid responsive genes by cellular retinoic acid binding protein-Ⅱ modulates RA mediated tumor cell proliferation and invasion[J]. Anticancer Res, 1998, 18(1A): 217-224.
7. 　Budhu AS, Noy N. Direct channeling of retinoic acid between cellular retinoic acid-binding proteinⅡ and retinoic acid receptor sensitizes mammary carcinoma cells to retinoic acid-induced growth arrest[J]. Mol Cell Biol, 2002, 22(8): 2632-2641.
8. 　Manor D, Shmidt EN, Budhu A, et al. Mammary carcinoma suppression by cellular retinoic acid binding protein-Ⅱ[J]. Cancer Res, 2003, 63(15): 4426-4433.
9. 　汤参娥, 李萃, 肖志强, 等. 人肺鳞癌组织的比较蛋白质组学研究[J]. 中华肿瘤杂志, 2006, 28(4): 274-279.
10. 　Fuj K, Kondo T, Yokoo H, et al. Proteomic study of human hepatocellular carcinoma using two-dimensional difference gel electrophoresis with saturation cysteine dye[J]. Proteomics, 2005, 5(5): 1411-1422.
11. 　Adamson J, Morgan EA, Beesley C, et al. High-level expression of cutaneous fatty acid-binding protein in prostatic carcinomas and its effect on tumorigenicity[J]. Oncogene, 2003, 22(18): 2739-2749.
12. 　Jing C, Beesley C, Foster CS, et al. Identification of the messenger RNA for human cutaneous fatty acid-binding protein as a metastasis inducer[J]. Cancer Res, 2000, 60(9): 2390-2398.
13. 　Jing C, Beesley C, Foster CS, et al. Human cutaneous fatty acid-binding protein induces metastasis by up-regulating the expression of vascular endothelial growth factor gene in rat Rama 37 model cells[J]. Cancer Res, 2001, 61(11): 4357-4364.
14. 　Bouzubar N, Walker KJ, Griffiths K, et al. Ki-67 immunostaining in primary breast cancer: pathological and clinical associations[J]. Br J Cancer, 1989, 59(6): 943-947.
15. 　Goldhirsch A, Ingle JN, Gelber RD, et al. Thresholds for therapies: highlights of the St Gallen International Expert Consensus on the primary therapy of early breast cancer 2009[J]. Ann Oncol, 2009, 20(8): 1319-1329.
16. 　唐平, 魏兵, 杨雯娟, 等. 乳腺癌预后/预测因子[J]. 中华病理学杂志, 2011, 40(2): 73-76.

West China Medical Journal

Expression of CRABPⅡ, E-FABP and Ki67 in Breast Invasive Ductal Carcinoma and Their Correlation

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