Experimental Study of Dexamethasone Treating in Non-freezing Cold Injury of Sciatic Nerves_West China Medical Journal

Authors：

 LIHao ¹ , XUMin ²

1. Department of Neurology, the First People's Hospital of Yibin, Yibin, Sichuan 644000, P. R. China;
2. Department of Neurology, Xuanwu Hospital of the Capital Medical University, Beijing, 100053, P. R. China;

Corresponding author：

Keywords：

Non-freezing cold injury; Sciatic nerve; Dexamethasone

DOI：

10.7507/1002-0179.20140068

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Objective To observe the changes of microstructure of rats'sciatic nerves with non-freezing cold injury after treated with dexamethasone. Methods Twelve male Wistar rats were randomly divided into cooling group and treating group.Unilateral sciatic nerves of the rats in the cooling group received cooling treatment with 3-5℃ for 2 hours;while unilateral sciatic nerves of the rats in the treating group received cooling treatment with 3-5℃ for 2 hours and underwent the celiac injection with dexamethasone in addition.The other sciatic nerves were exposed,as the control.The bilateral sciatic nerves of rats in each group were harvested after 24 hours.The microstructure of nerves was examined under the light microscope and electron microscope. Results Light microscopic examination revealed extensive myelinated fibre degeneration in form of giant empty axons or shrunken dark axons on the first day after cooled.And the endoneurial capillary lumen was narrowed because of swollen endothelial cells.After the treatment,myelinated fibre degeneration was still similar to that before the treatment,but the endoneurial capillary lumen and endothelial cells were normal.By electron microscopy,sciatic nerves showed extensive myelinated fiber degeneration,and swollen endothelial cells.But unmyelinated fibers and tight junction were preserved on the first days after cooled.Aggregated red cells and platelet thrombus were not found.After the treatment,myelinated fibre degeneration was still similar to that before the treatment.Unmyelinated fibers and tight junction were preserved.The endoneurial capillary lumen and endothelial cells were normal. Conclusion After the treatment,the damage of endoneurial capillary had improved,but myelinated fiber degeneration was similar to that before the treatment.It suggested that dexamethasone may only improve the vascular system in non-freezing cold injury of sciatic nerve.

Citation： LIHao, XUMin. Experimental Study of Dexamethasone Treating in Non-freezing Cold Injury of Sciatic Nerves. West China Medical Journal, 2014, 29(2): 219-222. doi: 10.7507/1002-0179.20140068 Copy

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1. 宋波,张涌泉.北方海岛部队新兵训练冻伤情况分析[J].白求恩军医学院学报,2008,6(6):363-364.
2. 应焱燕,朱银潮,徐荣,等.2008年宁波地区雨雪冰冻灾害所致伤害的流行病学调查[J].卫生研究,2009,38(4):463-464.
3. 刘玉红,杨伟娜,杨光元,等.高原高寒地区冻伤200例致残相关因素调查分析[J].临床军医杂志,2007,35(1):94-95.
4. Jia J,Pollock M.The pathogenesis of non-freezing cold nerve injury.Observations in the rat[J].Brain,1997,120(Pt 4):631-646.
5. Jia J,Pollock M.Cold nerve injury is enhanced by intermittent cooling[J].Muscle Nerve,1999,22(12):1644-1652.
6. Jia JP,Pollock M,Jia JM.Cold injury to nerves is not due to ischaemia alone[J].Brain,1998,121(Pt 5):989-1001.
7. 张锴,李积胜.寒冷环境对机体的影响及其机制[J].国外医学·卫生学分册,2006,33(4):212-215.
8. Dyck PJ,Giannini C.Pathologic alterations in the diabetic neuropathies of humans:a review[J].J Neuropathol Exp Neurol,1996,55(12):1181-1193.
9. Omura K,Ohbayashi M,Sano M,et al.The recovery of blood-nerve barrier in crush nerve injury--a quantitative analysis utilizing immunohistochemistry[J].Brain Res,2004,1001(1/2):13-21.
10. 耿志伟,徐敏,吴忧,等.氧自由基与大鼠坐骨神经非冻结性冷损伤[J].脑与神经疾病杂志,2008,16(5):598-601.
11. 耿志伟,张锦,佟晓燕,等.对坐骨神经局部温度变化过程中氧自由基的研究[J].黑龙江医学,2009,33(7):493-496.
12. 刘嘉瀛.氧自由基在冻伤发病中的作用[J].解放军预防医学杂志,1998,16(6):72-74.
13. Myers RR,Powell HC,Heckman HM,et al.Biophysical and Pathological effects of cryogenic nerve lesion[J].Ann Neurol,1981,10(5):478-485.
14. Olsson Y.Mast cells in the nervous system[J].Int Rev Cytol,1968,24:27-70.
15. 缪建云,练克俭.糖皮质激素治疗神经损伤的研究进展[J].中国骨与关节损伤杂志,2009,24(6):572-574.
16. 陈新谦,金有豫,汤光,等.新编药物学[M].北京:人民卫生出版社,1992:426.
17. 李浩,徐敏,耿志伟,等.巨噬细胞与坐骨神经非冻结性冷损伤[J].脑与神经疾病杂志,2010,18(6):432-435.

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