The Effect of Different Nutritional Diet on the Expression of IGFs and Its Receptor in Retarded Intrauterine Growth Rat Pups_West China Medical Journal

Authors：

 JIQiao-yun ¹ , ZHANGHui-dong ² , LUOShan ³ ,  MAOMeng ¹

1. Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Laboratory of Infection Immunity, Department of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
3. Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

MAOMeng, Email: dffmmao@126.com

Keywords：

Intrauterine growth retardation; IGFs; Rat; TLR4; Animal model

DOI：

10.7507/1002-0179.20140085

Video：

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Objective To investigate the effect of early different nutritional diet on the expression of IGFs and its receptor in retarded intrauterine growth rat pups. Methods The IUGR rat model was established by food restriction of pregnant rats.Newborn IUGR rats were randomly divided into four groups:IUGR model (S/N) group,IUGR high sugar diet (A) group,IUGR high protein diet (B) group,IUGR high fat (C) group.Only the mother rats were given those different diets individually,and all IUGR newborn pups were lactated for three weeks.All IUGR pups were fed with those different diets individually till the end of the month.Normal birth weight newborn rats were used as the control group fed with normal diet.Liver and lung tissues were detected by immunohistochemical staining and pancreatic tissues were stained with HE detection for all groups of rats. Results Polypeptide glycoprotein metalloprotease 12(ADAM12) test results each month showed no statistical difference (P>0.05).The determination results of plasma protein A (PAPP-A) in rat pups was statistically different among the groups in the first month (P<0.05),but no statistical difference was found in the second month,third month,and fourth month (P>0.05).TLR-4 test results had no statistical difference (P>0.05).Rat pancreatic tissue test results were statistically different in the first month (P<0.05),but the difference was not significant in the second month,third month,and fourth month (P>0.05). Conclusion ADAM-12 and pregnancy associated PAPP-A expression results suggest that ADAM12 and PAPP-A may be closely related with rat catch-up growth,while the TLR-4 test results are not statistically different,but the chances of high glucose group and high fat group pancreatic inflammation are relatively large.

Citation： JIQiao-yun, ZHANGHui-dong, LUOShan, MAOMeng. The Effect of Different Nutritional Diet on the Expression of IGFs and Its Receptor in Retarded Intrauterine Growth Rat Pups. West China Medical Journal, 2014, 29(2): 277-281. doi: 10.7507/1002-0179.20140085 Copy

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1. Salmon WD,Daughaday WH.A hormonally controlled serum factor which stimulates sulfate incorporation by cartilage in vitro[J].J Lab Clin Med,1957,49(6):825-836.
2. Setia S,Sridhar MG.Changes in GH/IGF-1 axis in intrauterine growth retardation:consequences of fetal programming [J].Horm Metab Res,2009,41(11):791-798.
3. Mullis PE,Tonella P.Regulation of fetal growth:consequences and impact of being born small[J].Best Pract Res Clin Endocrinol Metab,2008,22(1):173-190.
4. Sahraravand M,Järvelä IY,Laitinen P,et al.The secretion of PAPP-A,Adam12,and PP13 correlates with the size of the placenta for the first month of pregnancy[J].Placenta,2011,32(12):999-1003.
5. Valinen Y,Peuhkurinen S,Järvelä IY,et al.Maternal serum Adam12 levels correlate with PAPP-A levels during the first trimester[J].Gynecol Obstet Invest,2010,70(1):60-63.
6. Saraswathi V,Ramnanan CJ,Wilks AW,et al.Impact of hematopoietic cyclooxygenase-1 deficiency on obesity-linked adipose tissue inflammation and metabolic disorders in mice[J].Metabolism,2013,62(11):1673-1685.
7. Dong XS,Xu XY,Sun YQ,et al.Toll-like receptor 4 is involved in myocardial damage following paraquat poisoning in mice[J].Toxicology,2013,312:115-122.
8. Muetze S,Kapagerof A,Vlachopoulos L,et al.Mutation analysis of the growth factor genes PlGF,Flt1,IGF-I,and IGF-IR in intrauterine growth restriction with abnormal placental blood flow[J].J Matern Fetal Neonatal Med,2010,23(2):142-147.
9. Unterscheider J,Daly S,Geary MP,et al.Optimizing the definition of intrauterine growth restriction:the multicenter prospective Porto Study[J].Am J Obstet Gynecol,2013,208(4):290.e1-290.e6.
10. Lucas A.Programming by early nutrition:an experimental approach[J].J Nutr,1998,128(2 Suppl):401S-406S.
11. Xie L,Antonow-Schlorke I,Schwab M,et al.The frontal cortex IGF system is down regulated in the term,intrauterine growth restricted fetal baboon[J].Growth Horm IGF Res,2013,23(5):187-192.
12. Umbers AJ,Boeuf P,Clapham C,et al.Placental malaria-associated inflammation disturbs the insulin-like growth factor axis of fetal growth regulation[J].J Infect Dis,2011,203(4):561-569.
13. Lee MH,Jeon YJ,Lee SM,et al.Placental gene expression is related to glucose metabolism and fetal cord blood levels of insulin and insulin-like growth factors in intrauterine growth restriction[J].Early Hum Dev,2010,86(1):45-50.
14. Ozkan H,Aydin A,Demir N,et al.Associations of IGF-I,IGFBP-1 and IGFBP-3 on intrauterine growth and early catch-up growth[J].Biol Neonate,1999,76(5):274-282.
15. 李曼丽,甘华.高糖对肾小管上皮细胞Toll样受体4表达及其信号通路的影响[J].重庆医科大学学报,2010,35(6):857-859.
16. Carvajal AM,Huircan P,Lepori A.Single nucleotide polymorphisms in immunity-related genes and their association with mastitis in Chilean dairy cattle[J].Genet Mol Res,2013,12(3):2702-2711.
17. 崔晨,陈秋.TLR4信号转导途径与脂肪炎症、胰岛素抵抗的关系[J].山东医药,2009,49(27):109-110.
18. Nagai Y,Watanabe Y,Takatsu K.The TLR family protein RP105/MD-1 complex:a new player in obesity and adipose tissue inflammation[J].Adipocyte,2013,2(2):61-66.
19. 张放,李铁英,康健.TLR4在糖尿病大鼠肺组织表达的变化及对脂多糖反应性的研究[J].山东医药,2009,49(12):28-29.
20. Alyanakian MA,Grela F,Aumeunier A,et al.Transforming growth factor-beta and natural killer T-cells are involved in the protective effect of a bacterial extract on type 1 diabetes[J].Diabetes,2006,55(1):179-185.

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The Effect of Different Nutritional Diet on the Expression of IGFs and Its Receptor in Retarded Intrauterine Growth Rat Pups

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