Monitoring of Permanent Middle Cerebral Artery Occlusion Model in C57BL/6 Mice by Laser Doppler Flowmetry_West China Medical Journal

Authors：

 YANGQing , XUYi-ming , LIXiao-yu

Department of Pathophysiology, Nanjing Medical University/Provincial Laboratory of Molecular Intervention with Cardiovascular Diseases of Jiangsu, Nanjing, Jiangsu 210029, P. R. China;

Corresponding author：

Keywords：

Laser Doppler flowmetry; C57BL/6 mice; Permanent middle cerebral artery occlusion

DOI：

10.7507/1002-0179.20140263

Video：

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Objective To evaluate the feasibility of monitoring of permanent middle cerebral artery occlusion (MCAO) model in C57BL/6 mice by Laser Doppler flowmetry. Methods C57BL/6 mice were divided into 2 groups randomly:sham group and permanent MCAO group. Permanent MCAO model was established with the method of suture inserted into the internal carotid artery. Sufficiency of MCAO was monitored by Laser Doppler flowmetry during ischemia in mice. The neurological deficit score was assessed and the cerebral infarction size was measured by 2, 3, 5-triphenytetrazolium chloride (TTC) staining technique twenty-four hours after MCAO. Results In the MCAO group, the local blood flow was decreased from the preoperative value of (186.78±62.50) PU to the postoperative value of (25.80±7.66) PU. Cerebral blood flow was reduced by 90.4% during MCAO. The neurological deficit score was 2.48±0.36. The cerebral infarction area accounted for 39.79% by TTC staining. However, the cerebral blood flow fluctuations were not reduced and the neurological deficit score was found normal in the sham group. Furthermore, there was no cerebral infarction lesion in the sham group. Conclusion Monitoring by Laser Doppler flowmetry is efficient for evaluating the success rate of MCAO.

Citation： YANGQing, XUYi-ming, LIXiao-yu. Monitoring of Permanent Middle Cerebral Artery Occlusion Model in C57BL/6 Mice by Laser Doppler Flowmetry. West China Medical Journal, 2014, 29(5): 863-866. doi: 10.7507/1002-0179.20140263 Copy

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6.	刘宇, 孟然, 闫峰, 等. 激光多普勒血流仪评价活体大鼠大脑中动脉栓塞模型成功的可行性分析[J]. 中国病理生理杂志, 2011, 27(3):620-624.
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9.	Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1):84-91.
10.	Barone FC, Knudsen DJ, Nelson AH, et al. Mouse strain differences in susceptibility to cerebral ischemia are related to cerebral vascular anatomy[J]. J Cereb Blood Flow Metab, 1993, 13(4):683-692.
11.	Schmid-Elsaesser R, Zausinger S, Hungerhuber E, et al. A critical reevaluation of the intraluminal thread model of focal cerebral ischemia[J]. Stroke, 1998, 29(10):2162-2170.
12.	Yanamoto H, Nagata I, Niitsu Y, et al. Evaluation of MCAO stroke models in normotensive rats:standardized neocortical infarction by the 3VO technique[J]. Exp Neurol, 2003, 182(2):261-274.
13.	Harada H, Wang Y, Mishima Y, et al. A novel method of detecting rCBF with laser-Doppler flowmetry without cranial window through the skull for a MCAO rat model[J]. Brain Res Brain Res Protoc, 2005, 14(3):165-170.
14.	Xu Y, Qian L, Zong G, et al. Class a scavenger receptor promotes cerebral ischemic injury by pivoting microglia/macrophage polarization[J]. Neuroscience, 2012, 218:35-48.
15.	Henninger N, Bouley J, Bråtane BT, et al. Laser doppler flowmetry predicts occlusion but not tPA-mediated reperfusion success after rat embolic stroke[J]. Exp Neurol, 2009, 215(2):290-297.

1. Thom T, Haase N, Rosamond W, et al. Heart disease and stroke statistics--2006 update:a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee[J]. Circulation, 2006, 113(6):e85-e151.
2. Kim T, Masamoto K, Fukuda M, et al. Frequency-dependent neural activity, CBF, and BOLD fMRI to somatosensory stimuli in isoflurane-anesthetized rats[J]. Neuroimage, 2010, 52(1):224-233.
3. Masamoto K, Fukuda M, Vazquez A, et al. Dose-dependent effect of isoflurane on neurovascular coupling in rat cerebral cortex[J]. Eur J Neurosci, 2009, 30(2):242-250.
4. Lu Y, Zhang J, Ma B, et al. Glycine attenuates cerebral ischemia/reperfusion injury by inhibiting neuronal apoptosis in mice[J]. Neurochem Int, 2012, 61(5):649-658.
5. Schmid-Elsaesser R, Zausinger S, Hungerhuber E, et al. A critical reevaluation of the intraluminal thread model of focal cerebral ischemia:evidence of inadvertent premature reperfusion and subarachnoid hemorrhage in rats by laser-Doppler flowmetry[J]. Stroke, 1998, 29(10):2162-2170.
6. 刘宇, 孟然, 闫峰, 等. 激光多普勒血流仪评价活体大鼠大脑中动脉栓塞模型成功的可行性分析[J]. 中国病理生理杂志, 2011, 27(3):620-624.
7. Donnan GA, Fisher M, Macleod M, et al. Stroke[J]. Lancet, 2008, 371(9624):1612-1623.
8. Zhou L, Li F, Xu HB, et al. Treatment of cerebral ischemia by disrupting ischemia-induced interaction of nNOS with PSD-95[J]. Nat Med, 2010, 16(12):1439-1443.
9. Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1):84-91.
10. Barone FC, Knudsen DJ, Nelson AH, et al. Mouse strain differences in susceptibility to cerebral ischemia are related to cerebral vascular anatomy[J]. J Cereb Blood Flow Metab, 1993, 13(4):683-692.
11. Schmid-Elsaesser R, Zausinger S, Hungerhuber E, et al. A critical reevaluation of the intraluminal thread model of focal cerebral ischemia[J]. Stroke, 1998, 29(10):2162-2170.
12. Yanamoto H, Nagata I, Niitsu Y, et al. Evaluation of MCAO stroke models in normotensive rats:standardized neocortical infarction by the 3VO technique[J]. Exp Neurol, 2003, 182(2):261-274.
13. Harada H, Wang Y, Mishima Y, et al. A novel method of detecting rCBF with laser-Doppler flowmetry without cranial window through the skull for a MCAO rat model[J]. Brain Res Brain Res Protoc, 2005, 14(3):165-170.
14. Xu Y, Qian L, Zong G, et al. Class a scavenger receptor promotes cerebral ischemic injury by pivoting microglia/macrophage polarization[J]. Neuroscience, 2012, 218:35-48.
15. Henninger N, Bouley J, Bråtane BT, et al. Laser doppler flowmetry predicts occlusion but not tPA-mediated reperfusion success after rat embolic stroke[J]. Exp Neurol, 2009, 215(2):290-297.

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Monitoring of Permanent Middle Cerebral Artery Occlusion Model in C57BL/6 Mice by Laser Doppler Flowmetry

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