Experimental Study of Intrahepatic Biliary Perfusion by Contrast Enhanced Ultrasonography via Bile Duct_West China Medical Journal

Authors：

 WANGHong ¹ , LINGWen-wu ¹ , MALin ¹ , CHENKe-fei ² ,  LUQiang ¹

1. Department of Ultrasound, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Department of General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

LUQiang, Email: wsluqiang@126.com

Keywords：

Bile duct; Microbubble; Perfusion; Ultrasonic contrast

DOI：

10.7507/1002-0179.20150016

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the possibility of intrahepatic biliary perfusion by contrast enhanced ultrasonography via bile duct. Methods Sixteen rabbits weighing 2 000-2 500 g were studied. A 22 gauge catheter was inserted in the common bile duct by surgery. The rabbits were divided into 4 groups according to the concentration of contrast agent, including group A:1/100 of standard concentration; group B:1/200 of standard concentration; group C:1/400 of standard concentration; and group D:1/800 of standard concentration. We observed the filling status, presence of outflow of contrast enhanced signal and satisfactory enhanced time after the injection of contrast agent via the catheter in common bile duct. Results Five milliliter of contrast agent was injected in each rabbit. The number of homogeneous filling in group A, B, C and D was 4, 4, 3 and 0 respectively. The number of presence of outflow of contrast enhanced signal in group A, B, C and D was 4, 1, 0 and 0 respectively. The satisfactory enhanced time in group A, B, C and D was (340±29) s, (284±37) s, (82±8) s and 0 s respectively. There was no statistical difference in the satisfactory enhanced time between group A and B (P=0.06) while significant difference in the satisfactory enhanced time between group A and C (P < 0.01), and between group B and C (P < 0.01) was found. Conclusion It is possible to perfuse the intrahepatic biliary system by sonographic contrast agents via bile duct, and 1/200 of standard concentration is a proper concentration of contrast agent to achieve satisfactory imaging.

Citation： WANGHong, LINGWen-wu, MALin, CHENKe-fei, LUQiang. Experimental Study of Intrahepatic Biliary Perfusion by Contrast Enhanced Ultrasonography via Bile Duct. West China Medical Journal, 2015, 30(1): 60-62. doi: 10.7507/1002-0179.20150016 Copy

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11.	Xu HX. Contrast-enhanced ultrasound in the biliary system:potential uses and indications[J]. World J Radiol, 2009, 1(1):37-44.
12.	Xu HX, Lu MD, Liu LN, et al. Imaging features of intrahepatic biliary cystadenoma and cystadenocarcinoma on B-mode and contrast-enhanced ultrasound[J]. Ultraschall Med, 2012, 33(7):E241-E249.
13.	Ren J, Zheng BW, Wang P, et al. Revealing impaired blood supply to the bile ducts on contrast-enhanced ultrasound:a novel diagnosis method to ischemic-type biliary lesions after orthotropic liver transplantation[J]. Ultrasound Med Biol, 2013, 39(5):753-760.
14.	张艳玲, 许尔蛟, 郑荣琴, 等.经皮二维胆道超声造影的临床应用[J].中国医学影像技术, 2011, 27(6):1205-1208.
15.	蔡丽萍, 王琳, 赵克立, 等.胆管超声造影在肝内胆管置管引流术式选择中的应用价值[J].中华临床医师杂志:电子版, 2011, 5(8):2476-2477.
16.	Mao R, Xu EJ, Li K, et al. Usefulness of contrast-enhanced ultrasound in the diagnosis of biliary leakage following T-tube removal[J]. J Clin Ultrasound, 2010, 38(1):38-40.
17.	Xu EJ, Zheng RQ, Su ZZ, et al. Intra-biliary contrast-enhanced ultrasound for evaluating biliary obstruction during percutaneous transhepatic biliary drainage:a preliminary study[J]. Eur J Radiol, 2012, 81(12):3846-3850.
18.	许尔蛟, 郑荣琴, 李凯, 等.胆管内超声造影在经皮经肝胆道引流术中的应用价值[J].中华医学超声杂志:电子版, 2011, 8(9):1937-1945.
19.	郭欢仪, 许尔蛟, 郑荣琴, 等.术中胆道超声造影评价肝移植供体胆管解剖结构:二维与三维超声显像的比较[J].中华医学超声杂志:电子版, 2010, 7(10):1699-1705.

1. Claudon M, Dietrich CF, Choi B, et al. Guidelines and good clinical practice recommendations for contrast enhancedultrasound (CEUS) in the liver-update 2012:a WFUMB-EFSUMB initiative incooperation with representatives of AFSUMB, AIUM, ASUM, FLAUS and ICUS[J]. Ultraschall Med, 2013, 34(1):11-29.
2. Claudon M, Cosgrove D, Albrecht T, et al. Guidelines and good clinical practice recommendations for contrast enhanced ultrasound (CEUS)-update 2008[J]. Ultraschall Med, 2008, 29(1):28-44.
3. 戴晴, 姜玉新.超声造影的临床应用[J].中国医学科学院学报, 2008(1):1-4.
4. 周洋, 杜联芳.超声造影观察肝移植术后缺血性胆管病变的肝脏血流灌注[J].中国医学影像技术, 2010, 26(7):1273-1276.
5. 毛枫, 张兴伟, 李超伦, 等.周围型肝内胆管细胞癌:超声造影与增强CT对照[J].中国医学影像技术, 2013(9):1477-1480.
6. 毛枫, 李超伦, 黄备建, 等.肝内胆管细胞癌超声造影表现[J].中华医学超声杂志:电子版, 2013, 10(5):364-367.
7. 杨洁, 张学兰, 梅丽霞, 等.实时超声造影对肝外胆管癌的诊断价值[J].临床超声医学杂志, 2009, 11(10):689-691.
8. 徐辉雄, 刘广健, 吕明德, 等.肝内胆管癌超声造影的增强特点[J].中国超声医学杂志, 2006, 22(4):294-296.
9. Ren J, Lu MD, Zheng RQ, et al. Evaluation of the microcirculatory disturbance of biliary ischemia after liver transplantation with contrast-enhanced ultrasound:preliminary experience[J]. Liver Transpl, 2009, 15(12):1703-1708.
10. Meacock LM, Sellars ME, Sidhu PS. Evaluation of gallbladder and biliary duct disease using microbubble contrast-enhanced ultrasound[J]. Br J Radiol, 2010, 83(991):615-627.
11. Xu HX. Contrast-enhanced ultrasound in the biliary system:potential uses and indications[J]. World J Radiol, 2009, 1(1):37-44.
12. Xu HX, Lu MD, Liu LN, et al. Imaging features of intrahepatic biliary cystadenoma and cystadenocarcinoma on B-mode and contrast-enhanced ultrasound[J]. Ultraschall Med, 2012, 33(7):E241-E249.
13. Ren J, Zheng BW, Wang P, et al. Revealing impaired blood supply to the bile ducts on contrast-enhanced ultrasound:a novel diagnosis method to ischemic-type biliary lesions after orthotropic liver transplantation[J]. Ultrasound Med Biol, 2013, 39(5):753-760.
14. 张艳玲, 许尔蛟, 郑荣琴, 等.经皮二维胆道超声造影的临床应用[J].中国医学影像技术, 2011, 27(6):1205-1208.
15. 蔡丽萍, 王琳, 赵克立, 等.胆管超声造影在肝内胆管置管引流术式选择中的应用价值[J].中华临床医师杂志:电子版, 2011, 5(8):2476-2477.
16. Mao R, Xu EJ, Li K, et al. Usefulness of contrast-enhanced ultrasound in the diagnosis of biliary leakage following T-tube removal[J]. J Clin Ultrasound, 2010, 38(1):38-40.
17. Xu EJ, Zheng RQ, Su ZZ, et al. Intra-biliary contrast-enhanced ultrasound for evaluating biliary obstruction during percutaneous transhepatic biliary drainage:a preliminary study[J]. Eur J Radiol, 2012, 81(12):3846-3850.
18. 许尔蛟, 郑荣琴, 李凯, 等.胆管内超声造影在经皮经肝胆道引流术中的应用价值[J].中华医学超声杂志:电子版, 2011, 8(9):1937-1945.
19. 郭欢仪, 许尔蛟, 郑荣琴, 等.术中胆道超声造影评价肝移植供体胆管解剖结构:二维与三维超声显像的比较[J].中华医学超声杂志:电子版, 2010, 7(10):1699-1705.

West China Medical Journal

Experimental Study of Intrahepatic Biliary Perfusion by Contrast Enhanced Ultrasonography via Bile Duct

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