• 1. Department of Clinical Laboratory, Affiliated Hospital of Stomatology;
  • 2. Orofacial Reconstruction and Regeneration Laboratory;Luzhou Medical College, Luzhou, Sichuan 646000, P. R. China;
ZHENGLi-ge, Email: 541148817@qq.com
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Objective To explore the validity period of oral sterilizing instruments in different packages inside the bio-safety cabinet. Methods Inside the bio-safety cabinet, we brought the autoclaved dental gadgets packed in glassware and paper-plastic into microbial sampling cultivation experiment, and made observation of the bacteria growth at the 4th, 8th, 24th hour after sterilizing, with the sampling of once per 24 hours, lasting 10 days. Samples taken from the gadgets in paper-plastic each time when samples were taken from the gadgets in glassware were as the control. Results Under the condition that the bio-safety cabinet was not contaminated, no colony grew in neither group in 144 hours; 168 hours later, the cultivation results indicated that colonies started to grow(P<0.05). Conclusion Under the condition that the bio-safety cabinet is pollution-free, the validity period of gadgets in glassware packaging for dental clinic practices is far longer than the 4-hour limit after the sterilized packages are opened, as is stipulated in the "Operating Specifications for Sterilization Technology on Dental Instruments of Medical Institutions".

Citation: LIUDian-zhong, JIANGJun-qiang, GUOShi-li, LIUYao, ZHENGLi-ge. Experimental Study of the Influence of the Validity Period of Glassware Packaging on Sterilizing Instrument inside the Bio-safety Cabinet. West China Medical Journal, 2015, 30(2): 233-235. doi: 10.7507/1002-0179.20150072 Copy

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