The Expression of β-catenin and Galectin-3 in Cervical Carcinoma and Its Clinical Pathological Significances_West China Medical Journal

Authors：

 WUHe-gang , YUANSen-fu , ZHOUKai-mei , GUOYu , YUANMi , DONGHua-ju

Department of Pathology, the First People's Hospital of Yibin City, Yibin, Sichuan 644000, P. R. China;

Corresponding author：

Keywords：

β-catenin; Galectin-3; Cervical carcinoma; Invasion; Metastasis

DOI：

10.7507/1002-0179.20150416

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Objective To investigate the expression of β-catenin and Galectin-3 protein in human cervical carcinoma and its clinical pathological significance. Methods Eighty-three cervical specimens were collected from January 2010 to June 2013. By immunohistochemical method, β-catenin and Galectin-3 expression of the 83 cases of cervical carcinoma, 45 cases of intraepithelial neoplasia (CIN) and 25 normal cervix tissue (control) were detected. Results The positive expression rate in cervical carcinoma of β-catenin and Galectin-3 was respectively 74.70% and 81.93%, which was significantly higher than that in intraepithelial neoplasia (ⅠandⅡ) and normal cervical tissue (P<0.05). Compared with cervical cancer, the expression of β-catenin and Galectin-3 in CINⅢ had no statistical significance (P>0.05). The positive expression of β-catenin was significantly correlated with histological grade of cervical cancer tissue, International Federation of Gynecology and Obstetrics grade and lymph node metastasis (P<0.05). The positive expression of Galectin-3 was closely related to histological grade of cervical cancer tissue and lymph node metastasis (P>0.05). Both β-catenin and Galectin-3 expression had no relationship with other clinical pathological parameters, such as age of patients, tumor size and pathological pattern of tumor (P>0.05). β-catenin expression had significant positive correlation with that of Galectin-3 (r=0.327, P=0.002). Conclusion The expression of Galectin-3 and β-catenin increases obviously and is associated with abnormal clinical parameters (invasion or metastasis) in patients with cervical cancer. Galectin-3 and β-catenin may act as cancer metastasis and prognostic indicators in these patients.

Citation： WUHe-gang, YUANSen-fu, ZHOUKai-mei, GUOYu, YUANMi, DONGHua-ju. The Expression of β-catenin and Galectin-3 in Cervical Carcinoma and Its Clinical Pathological Significances. West China Medical Journal, 2015, 30(8): 1452-1456. doi: 10.7507/1002-0179.20150416 Copy

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2.	Kim SE, Huang H, Zhao M, et al. Wnt stabilization of β-catenin reveals principles for morphogen receptor-scaffold assemblies[J]. Science, 2013, 340(6134):867-870.
3.	Forouzanfar MH, Foreman KJ, Delossantos AM, et al. Breast and cervical cancer in 187 countries between 1980 and 2010:a systematic analysis[J]. Lancet, 2011, 378(9801):1461-1484.
4.	Chandrashekar C, Angadi PV, Krishnapillai R. β-Catenin expression in benign and malignant salivary gland tumors[J]. Int J Surg Pathol, 2011, 19(4):433-440.
5.	Ferrazzo KL, Neto MM, Dos Santos E, et al. Differential expression of galectin-3, beta-catenin, and cyclin D1 in adenoid cystic carcinoma and polymorphous low-grade adenocarcinoma of salivary glands[J]. J Oral Pathol Med, 2009, 38(9):701-707.
6.	Guha P, Kaptan E, Bandyopadhyaya G, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis[J]. Proc Natl Acad Sci USA, 2013, 110(13):5052-5057.
7.	De Oliveira JT, De Matos AJ, Gomes J, et al. Coordinated expression of galectin-3 and galectin-3-binding sites in malignant mammary tumors:implications for tumor metastasis[J]. Glycobiology, 2010, 20(11):1341-1352.
8.	Dakeng S, Duangmano S, Jiratchariyakul W, et al. Inhibition of Wnt signaling by cucurbitacin B in breast cancer cells:reduction of Wnt-associated proteins and reduced translocation of galectin-3-mediated β-catenin to the nucleus[J]. J Cell Biochem, 2012, 113(1):49-60.
9.	Pisani P, Bray F, Parkin DM. Estimates of the world-wide prevalence of cancer for 25 sites in the adult population[J]. Int J Cancer, 2002, 97(1):72-81.
10.	Sant'ana JM, Chammas R, Liu FT, et al. Activation of the Wnt/Beta-catenin signaling pathway during oral carcinogenesis process is not influenced by the absence of galectin-3 in mice[J]. Anticancer Res, 2011, 31(9):2805-2811.
11.	Jawhari AU, Farthing MJ, Pignatelli M. The E-cadherin/epidermal growth factor receptor interaction:a hypothesis of reciprocal and reversible control of intercellular adhesion and cell proliferation[J]. J Pathol, 1999, 187(2):155-157.
12.	Lee SB, Gong YD, Park YI, et al. 2,3,6-Trisubstituted quinoxaline derivative, a small molecule inhibitor of the Wnt/beta-catenin signaling pathway, suppresses cell proliferation and enhances radiosensitivity in A549/Wnt2 cells[J]. Biochem Biophys Res Commun, 2013, 431(4):746-752.
13.	Moody CA, Laimins LA. Human papillomavirus oncoproteins:pathways to transformation[J]. Nat Rev Cancer, 2010, 10(8):550-560.
14.	George S, Barysauskas C, Serrano C, et al. Retrospective cohort study evaluating the impact of intraperitoneal morcellation on outcomes of localized uterine leiomyosarcoma[J]. Cancer, 2014, 120(20):3154-3158.
15.	Chen J, Yao D, Li Y, et al. Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma[J]. Int J Mol Med, 2013, 32(3):557-567.
16.	Shu XS, Geng H, Li L, et al. The epigenetic modifier PRDM5 functions as a tumor suppressor through modulating WNT/β-catenin signaling and is frequently silenced in multiple tumors[J]. PLos One, 2011, 6(11):e27346.
17.	Samarzija I, Beard P. Hedgehog pathway regulators influence cervical cancer cell proliferation, survival and migration[J]. Biochem Biophys Res Commun, 2012, 425(1):64-69.

1. Valdespino VM, Valdespino VE. Cervical cancer screening:state of the art[J]. Curr Opin Obstet Gynecol, 2006, 18(1):35-40.
2. Kim SE, Huang H, Zhao M, et al. Wnt stabilization of β-catenin reveals principles for morphogen receptor-scaffold assemblies[J]. Science, 2013, 340(6134):867-870.
3. Forouzanfar MH, Foreman KJ, Delossantos AM, et al. Breast and cervical cancer in 187 countries between 1980 and 2010:a systematic analysis[J]. Lancet, 2011, 378(9801):1461-1484.
4. Chandrashekar C, Angadi PV, Krishnapillai R. β-Catenin expression in benign and malignant salivary gland tumors[J]. Int J Surg Pathol, 2011, 19(4):433-440.
5. Ferrazzo KL, Neto MM, Dos Santos E, et al. Differential expression of galectin-3, beta-catenin, and cyclin D1 in adenoid cystic carcinoma and polymorphous low-grade adenocarcinoma of salivary glands[J]. J Oral Pathol Med, 2009, 38(9):701-707.
6. Guha P, Kaptan E, Bandyopadhyaya G, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis[J]. Proc Natl Acad Sci USA, 2013, 110(13):5052-5057.
7. De Oliveira JT, De Matos AJ, Gomes J, et al. Coordinated expression of galectin-3 and galectin-3-binding sites in malignant mammary tumors:implications for tumor metastasis[J]. Glycobiology, 2010, 20(11):1341-1352.
8. Dakeng S, Duangmano S, Jiratchariyakul W, et al. Inhibition of Wnt signaling by cucurbitacin B in breast cancer cells:reduction of Wnt-associated proteins and reduced translocation of galectin-3-mediated β-catenin to the nucleus[J]. J Cell Biochem, 2012, 113(1):49-60.
9. Pisani P, Bray F, Parkin DM. Estimates of the world-wide prevalence of cancer for 25 sites in the adult population[J]. Int J Cancer, 2002, 97(1):72-81.
10. Sant'ana JM, Chammas R, Liu FT, et al. Activation of the Wnt/Beta-catenin signaling pathway during oral carcinogenesis process is not influenced by the absence of galectin-3 in mice[J]. Anticancer Res, 2011, 31(9):2805-2811.
11. Jawhari AU, Farthing MJ, Pignatelli M. The E-cadherin/epidermal growth factor receptor interaction:a hypothesis of reciprocal and reversible control of intercellular adhesion and cell proliferation[J]. J Pathol, 1999, 187(2):155-157.
12. Lee SB, Gong YD, Park YI, et al. 2,3,6-Trisubstituted quinoxaline derivative, a small molecule inhibitor of the Wnt/beta-catenin signaling pathway, suppresses cell proliferation and enhances radiosensitivity in A549/Wnt2 cells[J]. Biochem Biophys Res Commun, 2013, 431(4):746-752.
13. Moody CA, Laimins LA. Human papillomavirus oncoproteins:pathways to transformation[J]. Nat Rev Cancer, 2010, 10(8):550-560.
14. George S, Barysauskas C, Serrano C, et al. Retrospective cohort study evaluating the impact of intraperitoneal morcellation on outcomes of localized uterine leiomyosarcoma[J]. Cancer, 2014, 120(20):3154-3158.
15. Chen J, Yao D, Li Y, et al. Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma[J]. Int J Mol Med, 2013, 32(3):557-567.
16. Shu XS, Geng H, Li L, et al. The epigenetic modifier PRDM5 functions as a tumor suppressor through modulating WNT/β-catenin signaling and is frequently silenced in multiple tumors[J]. PLos One, 2011, 6(11):e27346.
17. Samarzija I, Beard P. Hedgehog pathway regulators influence cervical cancer cell proliferation, survival and migration[J]. Biochem Biophys Res Commun, 2012, 425(1):64-69.

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