蜡状芽孢杆菌分泌的致吐毒素对胰岛β细胞毒性作用研究进展_West China Medical Journal

Authors：

 都建苹 ,  安振梅

四川大学华西医院内分泌代谢科 (成都 610041);

Corresponding author：

安振梅, Email: anzm1997@sina.com

Keywords：

蜡状芽孢杆菌; 致吐毒素; 食品污染; 糖尿病; 胰岛β细胞

DOI：

10.7507/1002-0179.201600211

Video：

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糖尿病是一种常见病、多发病，其患病率呈上升趋势，且认为这种上升趋势与环境因素有关，但具体的影响因素目前尚不明确。特定蜡状芽孢杆菌株分泌的致吐毒素主要存在于淀粉类食物中，由于其耐热、耐酸及不易被蛋白水解酶破坏的特性，使其很难从食物及消化道清除。致吐毒素具有钾离子载体的特性，通过破坏胰岛β细胞线粒体跨膜电位导致胰岛素分泌减少以及细胞凋亡。体外研究显示，极低剂量的致吐毒素也具有毒性作用，而胰岛β细胞对其尤为敏感。然而目前尚缺乏相关的体内研究，未来亟需进行相关体内研究，以进一步阐明致吐毒素与糖尿病发生的相关性。

Citation： 都建苹, 安振梅. 蜡状芽孢杆菌分泌的致吐毒素对胰岛β细胞毒性作用研究进展. West China Medical Journal, 2016, 31(4): 771-774. doi: 10.7507/1002-0179.201600211 Copy

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18.	Paananen A, Mikkola R, Sareneva T, et al. Inhibition of human natural killer cell activity by cereulide, an emetic toxin from Bacillus cereus[J]. Clin Exp Immunol, 2002, 129(3): 420-428.
19.	Maechler P, Carobbio S, Rubi B. In beta-cells, mitochondria integrate and generate metabolic signals controlling insulin secretion[J]. Int J Biochem Cell Biol, 2006, 38(5/6): 696-709.
20.	Koster JC, Nichols CG. Diabetes and insulin secretion: the ATP-sensitive K⁺ channel (K_ATP) connection[J]. Diabetes, 2005, 54(11): 3065-3072.
21.	Gogvadze V. Mitochondrial cytochrome c release may occur by volume-dependent mechanisms not involving permeability transition[J]. Biochem J, 2004, 378(Pt 1): 213-217.
22.	Andersson MA, Hakulinen P, Honkalampi-Hamalainen U, et al. Toxicological profile of cereulide, the Bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells[J]. Toxicon, 2007, 49(3): 351-367.
23.	Teplova V, Jaaskelainen E, Salkinoja-Salonen M, et al. Differentiated Paju cells have increased resistance to toxic effects of potassium ionophores[J]. Acta Biochim Pol, 2004, 51(2): 539-544.
24.	Rajkovic A, Grootaert C, Butorac A, et al. Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells[J]. Toxins (Basel), 2014, 6(8): 2270-2290.
25.	Hu EA, Pan A, Malik V, et al. White rice consumption and risk of type 2 diabetes: meta-analysis and systematic review[J]. BMJ, 2012, 344: e1454.
26.	Delbrassinne L, Botteldoorn N, Andjelkovic M, et al. An emetic Bacillus cereus outbreak in a kindergarten: detection and quantification of critical levels of cereulide toxin[J]. Foodborne Pathog Dis, 2015, 12(1): 84-87.
27.	张静, 吕毅. 肠道菌群失调诱发2型糖尿病的研究进展[J]. 中国微生态学杂志, 2016, 28(1): 113-116.

1. Halban PA, Polonsky KS, Bowden DW, et al. beta-cell failure in type 2 diabetes: postulated mechanisms and prospects for prevention and treatment[J]. Diabetes Care, 2014, 37(6): 1751-1758.
2. Hectors T, Vanparys C, Van Der Ven K, et al. Environmental pollutants and type 2 diabetes: a review of mechanisms that can disrupt beta cell function[J]. Diabetologia, 2011, 54(6): 1273-1290.
3. Vangoitsenhoven R, Maris M, Overbergh L, et al. Cereulide food toxin, beta cell function and diabetes: facts and hypotheses[J]. Diabetes Res Clin Pract, 2015, 109(1): 1-5.
4. Apetroaie C, Andersson MA, Spröer C, et al. Cereulide-producing strains of Bacillus cereus show diversity[J]. Arch Microbiol, 2005, 184(3): 141-151.
5. Briley RT, Fowler JP. Nontypical Bacillus cereus outbreak in a child care center[J]. J Environ Health, 2001, 63(7): 9-11.
6. Messelhausser U. Emetic Bacillus cereus are more volatile than thought: recent foodborne outbreaks and prevalence studies in Bavaria (2007-2013)[J]. Biomed Res Int, 2014, 2014: 465603.
7. Vangoitsenhoven R, Rondas D, Crèvecoeur I, et al. Foodborne cereulide causes beta-cell dysfunction and apoptosis[J]. PLoS One, 2014, 9(8): e104866.
8. Agata, N. A novel dodecadepsipeptide, cereulide, isolated from Bacillus cereus causes vacuole formation in HEp-2 cells[J]. FEMS Microbiol Lett, 1994, 121(1): 31-34.
9. Kroten MA, Swiecicka I. Cereulide and valinomycin, two important natural dodecadepsipeptides with ionophoretic activities[J]. Pol J Microbiol, 2010, 59(1): 3-10.
10. Virtanen SM, Roivainen M, Andersson MA, et al. In vitro toxicity of cereulide on porcine pancreatic Langerhans islets[J]. Toxicon, 2008, 51(6): 1029-1037.
11. Shiota M, Saitou K, Mizumoto H, et al. Rapid detoxification of cereulide in Bacillus cereus food poisoning[J]. Pediatrics, 2010, 125(4): e951-e955.
12. Dierick K, Van Coillie E, Swiecicka I, et al. Fatal family outbreak of Bacillus cereus-associated food poisoning[J]. J Clin Microbiol, 2005, 43(8): 4277-4279.
13. Mahler H, Pasi A, Kramer JM, et al. Fulminant liver failure in association with the emetic toxin of Bacillus cereus[J]. N Engl J Med, 1997, 336(16): 1142-1148.
14. Naranjo M, Denayer S, Botteldoorn NA, et al. Sudden death of a young adult associated with Bacillus cereus food poisoning[J]. J Clin Microbiol, 2011, 49(12): 4379-4381.
15. Teplova VV, Mikkola R, Tonshin AA, et al. The higher toxicity of cereulide relative to valinomycin is due to its higher affinity for potassium at physiological plasma concentration[J]. Toxicol Appl Pharmacol, 2006, 210(1/2): 39-46.
16. Popoff MR, Poulain B. Bacterial toxins and the nervous system: neurotoxins and multipotential toxins interacting with neuronal cells[J]. Toxins (Basel), 2010, 2(4): 683-737.
17. Hoornstra D, Andersson MA, Teplova VV, et al. Potato crop as a source of emetic Bacillus cereus and cereulide-induced mammalian cell toxicity[J]. Appl Environ Microbiol, 2013, 79(12): 3534-3543.
18. Paananen A, Mikkola R, Sareneva T, et al. Inhibition of human natural killer cell activity by cereulide, an emetic toxin from Bacillus cereus[J]. Clin Exp Immunol, 2002, 129(3): 420-428.
19. Maechler P, Carobbio S, Rubi B. In beta-cells, mitochondria integrate and generate metabolic signals controlling insulin secretion[J]. Int J Biochem Cell Biol, 2006, 38(5/6): 696-709.
20. Koster JC, Nichols CG. Diabetes and insulin secretion: the ATP-sensitive K⁺ channel (K_ATP) connection[J]. Diabetes, 2005, 54(11): 3065-3072.
21. Gogvadze V. Mitochondrial cytochrome c release may occur by volume-dependent mechanisms not involving permeability transition[J]. Biochem J, 2004, 378(Pt 1): 213-217.
22. Andersson MA, Hakulinen P, Honkalampi-Hamalainen U, et al. Toxicological profile of cereulide, the Bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells[J]. Toxicon, 2007, 49(3): 351-367.
23. Teplova V, Jaaskelainen E, Salkinoja-Salonen M, et al. Differentiated Paju cells have increased resistance to toxic effects of potassium ionophores[J]. Acta Biochim Pol, 2004, 51(2): 539-544.
24. Rajkovic A, Grootaert C, Butorac A, et al. Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells[J]. Toxins (Basel), 2014, 6(8): 2270-2290.
25. Hu EA, Pan A, Malik V, et al. White rice consumption and risk of type 2 diabetes: meta-analysis and systematic review[J]. BMJ, 2012, 344: e1454.
26. Delbrassinne L, Botteldoorn N, Andjelkovic M, et al. An emetic Bacillus cereus outbreak in a kindergarten: detection and quantification of critical levels of cereulide toxin[J]. Foodborne Pathog Dis, 2015, 12(1): 84-87.
27. 张静, 吕毅. 肠道菌群失调诱发2型糖尿病的研究进展[J]. 中国微生态学杂志, 2016, 28(1): 113-116.

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West China Medical Journal

蜡状芽孢杆菌分泌的致吐毒素对胰岛β细胞毒性作用研究进展

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