Expression and Role of Adrenomedullin in Patients with Liver Cancer_West China Medical Journal

Authors：

 YaoYang , SuJie , XuRui , ZhuXingmei , ChengBiping

Central Laboratory, the First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710077, P. R. China;

Corresponding author：

Keywords：

Liver cancer; Adrenomedullin; Immunohistochemistry; Enzyme-linked immunosorbent assay

DOI：

10.7507/1002-0179.20160072

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Objective To investigate the expression of adrenomedullin (ADM) in human liver cancer and to evaluate its clinical signigicance. Methods A total of 54 patients with liver cancer who underwent surgery between January 2012 and November 2013 and another 18 benigh tissue samples were selected. Immuohistochemical staining (SP staining) was performed to detect the expression of ADM in liver cancer, and enzyme-linked immunosorbent assay was applied to determine the concentration of ADM in the patients and controls, and the relationship was retrospectively analyzed between the expression of ADM in liver cancer and the clinicobiological features. Besides, 60 inpatients with liver cancer and 40 health people under went physical examination between January and June 2013 were selected, whose expression of ADM in plasma was deteced. Results The expression of ADM in liver cancer was significantly higher than that in the normal tissues (P<0.01). There was a significant relationship between the expression of ADM and the differentiation grade in liver cancer (P<0.05), and there was no relationship between the expression of ADM and age, histological type and American Joint Committen on Cancer (AJCC) stage (P>0.05). The expression of ADM in the plasma in liver cancer group was significantly higher than that in the control group (P<0.05). There was a significant relationship between the expression of ADM in the plasma and AJCC stage (P<0.05). Conclusion The expression of ADM significantly increases in patients with liver cancer, which is associated with the differentiation grades of the liver cancer. ADM may play an important role in the occurrence and development of liver cancer.

Citation： YaoYang, SuJie, XuRui, ZhuXingmei, ChengBiping. Expression and Role of Adrenomedullin in Patients with Liver Cancer. West China Medical Journal, 2016, 31(2): 266-269. doi: 10.7507/1002-0179.20160072 Copy

1.	Kitamura K, Kangawa K, Kawamoto M, et al. Adrenomedullin:a novel hypotensive peptide isolated from human pheochromocytoma[J]. Biochem Biophys Res Commun, 1993, 192(2):553-560.
2.	Ribatti D, Nico B, Spinazzi R, et al. The role of adrenomedullin in angiogenesis[J]. Peptides, 2005, 26(9):1670-1675.
3.	Zudaire E, Martínez A, Cuttitta F. Adrenomedullin and cancer[J]. Regul Pept, 2003, 112(1/2/3):175-183.
4.	李云涛, 崔娜, 陈丽芬, 等. 乳腺癌中肾上腺髓质素的表达及其与癌组织分化、转移间的关系[J]. 中国肿瘤生物治疗杂志, 2009, 16(1):80-83.
5.	Hong YG, Hay DL, Quirion R, et al. The pharmacology of adrenomedullin 2/intermedin[J]. Br J Pharmacol, 2012, 166(1):110-120.
6.	Kuwasako K, Kitamura K, Nagata S, et al. Shared and separate functions of the RAMP-based adrenomedullin receptors[J]. Peptides, 2011, 32(7):1540-1550.
7.	Edge SB, Byrd DR, Comptom CC, et al. AJCC cancer staging manual[M]. 7th ed. New York:Springer, 2009:211-214.
8.	Hanazaki K, Kajikawa S, Shimozawa N, et al. Survival and recurrence after hepatic resection of 386 consecutive patients with hepatocellular carcinoma[J]. J Am Coll Surg, 2000, 191(4):381-388.
9.	Bruix J, Sherman M. Management of hepatocellular carcinoma[J]. Hepatology, 2005, 42(5):1208-1236.
10.	Yang JD, Lewis RR. Hepatocellular carcinoma:a global view[J]. Nature Rev Gastroenterol Hepatol, 2010, 7(8):448-458.
11.	Ascha MS, Hanouneh IA, Lopez R, et al. The incidence and risk factors of hepatocellular carcinoma in patients with nonalcoholic steatohepatitis[J]. Hepatology, 2010, 51(6):1972-1978.
12.	Miller MJ, Martínez A, Unsworth EJ, et al. Adrenomedullin expression in human tumor cell lines. Its potential role as an autocrine growth factor[J]. J Biol Chem, 1996, 271(38):23345-23351.
13.	Hata K, Takebayashi Y, Akiba S, et al. Expression of the adrenomedullin gene in epithelialov-varian cancer[J]. Mol Hum Reprod, 2000, 6(10):867-872.
14.	Albertin G, Carraro G, Petrelli L, et al. Endothelin-1 and adrenomedullin enhance the growth of human adrenocortical carcinoma-derived SW-13 cell line by stimulating proliferation and inhibiting apoptosis[J]. Int J Mol Med, 2005, 15(3):469-474.
15.	Rocchi P, Boudouresque F, Zamora AJ, et al. Expression of adrenomedullin and peptide amidation activity in human prostate cancer and in human prostate cancer cell lines[J]. Cancer Res, 2001, 61(3):1196-1206.
16.	Quafik L, Sauze S, Boudouresque F, et al. Neutralization of adrenomedullin inhibits the growth of human glioblastoma cell lines in vivo and suppresses tumor xenograft growth in vivo[J]. Am J Pathol, 2002, 160(4):1279-1292.
17.	温雪萍, 李艳, 王忠慧, 等. 肾上腺髓质素在人喉癌组织中的表达[J]. 蚌埠医学院学报, 2009, 34(6):473-476.

1. Kitamura K, Kangawa K, Kawamoto M, et al. Adrenomedullin:a novel hypotensive peptide isolated from human pheochromocytoma[J]. Biochem Biophys Res Commun, 1993, 192(2):553-560.
2. Ribatti D, Nico B, Spinazzi R, et al. The role of adrenomedullin in angiogenesis[J]. Peptides, 2005, 26(9):1670-1675.
3. Zudaire E, Martínez A, Cuttitta F. Adrenomedullin and cancer[J]. Regul Pept, 2003, 112(1/2/3):175-183.
4. 李云涛, 崔娜, 陈丽芬, 等. 乳腺癌中肾上腺髓质素的表达及其与癌组织分化、转移间的关系[J]. 中国肿瘤生物治疗杂志, 2009, 16(1):80-83.
5. Hong YG, Hay DL, Quirion R, et al. The pharmacology of adrenomedullin 2/intermedin[J]. Br J Pharmacol, 2012, 166(1):110-120.
6. Kuwasako K, Kitamura K, Nagata S, et al. Shared and separate functions of the RAMP-based adrenomedullin receptors[J]. Peptides, 2011, 32(7):1540-1550.
7. Edge SB, Byrd DR, Comptom CC, et al. AJCC cancer staging manual[M]. 7th ed. New York:Springer, 2009:211-214.
8. Hanazaki K, Kajikawa S, Shimozawa N, et al. Survival and recurrence after hepatic resection of 386 consecutive patients with hepatocellular carcinoma[J]. J Am Coll Surg, 2000, 191(4):381-388.
9. Bruix J, Sherman M. Management of hepatocellular carcinoma[J]. Hepatology, 2005, 42(5):1208-1236.
10. Yang JD, Lewis RR. Hepatocellular carcinoma:a global view[J]. Nature Rev Gastroenterol Hepatol, 2010, 7(8):448-458.
11. Ascha MS, Hanouneh IA, Lopez R, et al. The incidence and risk factors of hepatocellular carcinoma in patients with nonalcoholic steatohepatitis[J]. Hepatology, 2010, 51(6):1972-1978.
12. Miller MJ, Martínez A, Unsworth EJ, et al. Adrenomedullin expression in human tumor cell lines. Its potential role as an autocrine growth factor[J]. J Biol Chem, 1996, 271(38):23345-23351.
13. Hata K, Takebayashi Y, Akiba S, et al. Expression of the adrenomedullin gene in epithelialov-varian cancer[J]. Mol Hum Reprod, 2000, 6(10):867-872.
14. Albertin G, Carraro G, Petrelli L, et al. Endothelin-1 and adrenomedullin enhance the growth of human adrenocortical carcinoma-derived SW-13 cell line by stimulating proliferation and inhibiting apoptosis[J]. Int J Mol Med, 2005, 15(3):469-474.
15. Rocchi P, Boudouresque F, Zamora AJ, et al. Expression of adrenomedullin and peptide amidation activity in human prostate cancer and in human prostate cancer cell lines[J]. Cancer Res, 2001, 61(3):1196-1206.
16. Quafik L, Sauze S, Boudouresque F, et al. Neutralization of adrenomedullin inhibits the growth of human glioblastoma cell lines in vivo and suppresses tumor xenograft growth in vivo[J]. Am J Pathol, 2002, 160(4):1279-1292.
17. 温雪萍, 李艳, 王忠慧, 等. 肾上腺髓质素在人喉癌组织中的表达[J]. 蚌埠医学院学报, 2009, 34(6):473-476.

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Expression and Role of Adrenomedullin in Patients with Liver Cancer

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