Expression of CD34 and polyclone carcinoembryonic antigen detected by puncture biopsy of positive and negative alpha fetoprotein in human hepatocellular carcinoma tissue and its significance of pathological diagnosis_West China Medical Journal

Authors：

LI Wei , WANG Hongli , LI Hong , CHEN Su , YU Meiying , ZHOU Qiuyuan , XU Yanhua ,  QU Yangang

Department of Pathology, the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei 445000, P. R. China;

Corresponding author：

QU Yangang, Email: 1324322271@qq.com

Keywords：

Hepatocellular carcinoma; Alpha fetoprotein; CD34; Polyclone carcinoembryonic antigen; Immunohistochemistry; Pathological diagnosis

DOI：

10.7507/1002-0179.201606041

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore the expression of CD34 and polyclone carcinoembryonic antigen (pCEA) of positive and negative alpha fetoprotein (AFP) detected by puncture biopsy in human hepatocellular carcinoma (HCC) and the significance of pathological diagnosis. Methods Fifty-four HCC tissue specimens from 2013 to 2015 were collected from tumor biopsy samples which confirmed by pathology in the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture. The tissue samples were divided into positive AFP group (n=33) and negative AFP group (n=21) according to the detection results of serology and immunohistochemistry analysis of AFP. Expressions of CD34 and pCEA in the fifty-four HCC specimens were detected by immunohistochemistry. Results The positvie expression rate of pCEA in the positive AFP group was 69.7%, which was significantly higher than that in the negative AFP group (38.1%) (P<0. 05). However, the difference in positive expression rate of CD34 between the positive and negative AFP groups (90.91% and 85.71%, respectively) was not significant (P>0.05). Conclusion The associated detection of AFP, pCEA and CD34 in HCC tissues might contribute to the pathological and differential diagnosis of human hepatocellular carcinoma in puncture biopsies.

Citation： LI Wei, WANG Hongli, LI Hong, CHEN Su, YU Meiying, ZHOU Qiuyuan, XU Yanhua, QU Yangang. Expression of CD34 and polyclone carcinoembryonic antigen detected by puncture biopsy of positive and negative alpha fetoprotein in human hepatocellular carcinoma tissue and its significance of pathological diagnosis. West China Medical Journal, 2017, 32(8): 1195-1198. doi: 10.7507/1002-0179.201606041 Copy

1.	El-serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology, 1994, 132(7): 25571-2576.
2.	林黎娟, 金政, 王研, 等. 超声引导下肝癌穿刺组织中 DEK 表达的临床意义. 辽东学院学报: 自然科学版, 2011, 18(1): 50-52, 58.
3.	Ahmad M, Riley TR. Can one predict when ultrasound will be useful with percutaneous liver biopsy? Am J Gastroenterol, 2001, 96(2): 547-549.
4.	欧阳茂, 李锡寿, 李会斌, 等. 肝穿刺活检经验总结——附 949 例分析. 上海医学, 1999, 22(3): 189.
5.	丛文铭, 谭璐, 张树辉, 等. 肝癌免疫组化诊断谱的研究和应用. 中华肿瘤杂志, 2002, 24(6): 553-556.
6.	闫伟伟, 黄昂, 李永纲, 等. CD34 和 CD117 在人肝癌组织中的表达及其临床意义. 中华肝脏病杂志, 2011, 19(8): 588-593.
7.	左婷婷, 郑荣寿, 曾红梅, 等. 中国肝癌发病状况与趋势分析. 中华肿瘤杂志, 2015, 37(9): 691-696.
8.	Srivatanakul P, Sriplung H, Deerasamee S. Epidemiology of liver cancer: an overview. Asian Pac J Cancer Prev, 2004, 5(2): 118-125.
9.	But DY, Lai CL, Yuen MF. Natural history of hepatitis-related hepatocellular carcinoma. World J Gastroenterol, 2008, 14(11): 1652-1656.
10.	陈建国, 陆建华, 朱源荣, 等. 乙型肝炎病毒感染与肝癌发生的 31 年随访研究. 中华流行病学杂志, 2010, 31(7): 721-726.
11.	Lau SK, Prakash S, Geller SA, et al. Comparative immunohistochemical profile of hepatocellular carcinoma, cholangiocarcinoma, and metastatic adenocarcinoma. Hum Pathol, 2002, 33(12): 1175-1181.
12.	范公忍, 汪毅, 胡大荣. TSGF、AFP、SF 联合检测对原发性肝癌诊断意义的评价. 上海免疫学杂志, 2000, 20(5): 317-317.
13.	丛文铭, 朱世能. 肝胆肿瘤诊断外科病理学. 上海: 上海科技教育出版社, 2002: 132-164.
14.	Soresi M, Magliarisi C, Campagna P, et al. Usefulness of alpha-fetoprotein in the diagnosis of hepatocellular carcinoma. Anticancer Res, 2003, 23(2C): 1747-1753.
15.	Zimmerman RL, Logani S, Baloch Z. Evaluation of the CD34 and CD10 immunostains using a two-color staining protocol in liver fine-needle aspiration biopsies. Diagn Cytopathol, 2005, 32(2): 88-91.
16.	Murakata LA, Ishak KG, Nzeako UC. Clear cell carcinoma of the liver: a comparative immunohistochemical study with renal clear cell carcinoma. Mod Pathol, 2000, 13(8): 874-881.
17.	Ohmori S, Shiraki K, Sugimoto K, et al. High expression of CD34-positive sinusoidal endothelial cells is a risk factor for hepatocellular carcinoma in patients with HCV-associated chronic liver diseases. Hum Pathol, 2001, 32(12): 1363-1370.

1. El-serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology, 1994, 132(7): 25571-2576.
2. 林黎娟, 金政, 王研, 等. 超声引导下肝癌穿刺组织中 DEK 表达的临床意义. 辽东学院学报: 自然科学版, 2011, 18(1): 50-52, 58.
3. Ahmad M, Riley TR. Can one predict when ultrasound will be useful with percutaneous liver biopsy? Am J Gastroenterol, 2001, 96(2): 547-549.
4. 欧阳茂, 李锡寿, 李会斌, 等. 肝穿刺活检经验总结——附 949 例分析. 上海医学, 1999, 22(3): 189.
5. 丛文铭, 谭璐, 张树辉, 等. 肝癌免疫组化诊断谱的研究和应用. 中华肿瘤杂志, 2002, 24(6): 553-556.
6. 闫伟伟, 黄昂, 李永纲, 等. CD34 和 CD117 在人肝癌组织中的表达及其临床意义. 中华肝脏病杂志, 2011, 19(8): 588-593.
7. 左婷婷, 郑荣寿, 曾红梅, 等. 中国肝癌发病状况与趋势分析. 中华肿瘤杂志, 2015, 37(9): 691-696.
8. Srivatanakul P, Sriplung H, Deerasamee S. Epidemiology of liver cancer: an overview. Asian Pac J Cancer Prev, 2004, 5(2): 118-125.
9. But DY, Lai CL, Yuen MF. Natural history of hepatitis-related hepatocellular carcinoma. World J Gastroenterol, 2008, 14(11): 1652-1656.
10. 陈建国, 陆建华, 朱源荣, 等. 乙型肝炎病毒感染与肝癌发生的 31 年随访研究. 中华流行病学杂志, 2010, 31(7): 721-726.
11. Lau SK, Prakash S, Geller SA, et al. Comparative immunohistochemical profile of hepatocellular carcinoma, cholangiocarcinoma, and metastatic adenocarcinoma. Hum Pathol, 2002, 33(12): 1175-1181.
12. 范公忍, 汪毅, 胡大荣. TSGF、AFP、SF 联合检测对原发性肝癌诊断意义的评价. 上海免疫学杂志, 2000, 20(5): 317-317.
13. 丛文铭, 朱世能. 肝胆肿瘤诊断外科病理学. 上海: 上海科技教育出版社, 2002: 132-164.
14. Soresi M, Magliarisi C, Campagna P, et al. Usefulness of alpha-fetoprotein in the diagnosis of hepatocellular carcinoma. Anticancer Res, 2003, 23(2C): 1747-1753.
15. Zimmerman RL, Logani S, Baloch Z. Evaluation of the CD34 and CD10 immunostains using a two-color staining protocol in liver fine-needle aspiration biopsies. Diagn Cytopathol, 2005, 32(2): 88-91.
16. Murakata LA, Ishak KG, Nzeako UC. Clear cell carcinoma of the liver: a comparative immunohistochemical study with renal clear cell carcinoma. Mod Pathol, 2000, 13(8): 874-881.
17. Ohmori S, Shiraki K, Sugimoto K, et al. High expression of CD34-positive sinusoidal endothelial cells is a risk factor for hepatocellular carcinoma in patients with HCV-associated chronic liver diseases. Hum Pathol, 2001, 32(12): 1363-1370.

Previous Article
Association between Caveolin-1 protein expression and gastric cancer as well as its clinical pathologic features: a Meta-analysis
Next Article
Clinical features and risk factors of diabetic foot

West China Medical Journal

Expression of CD34 and polyclone carcinoembryonic antigen detected by puncture biopsy of positive and negative alpha fetoprotein in human hepatocellular carcinoma tissue and its significance of pathological diagnosis

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content