The impact of Liuhedan on interleukin-1β and tumor necrosis factor-alpha during infective wound healing in rabbits_West China Medical Journal

Authors：

LÜ Changyao , WU Jing ,  HE Hongbo

Department of Integrated Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

HE Hongbo, Email: 18980601615@163.com

Keywords：

Liuhedan; Wound healing; Interleukin-1β; Tumor necrosis factor-α

DOI：

10.7507/1002-0179.201703100

Video：

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Objective To explore the mechanism of Liuhedan in promoting wound healing through applying Liuhedan to the infective wounds of New Zealand white rabbits. Methods A total of forty New Zealand white rabbit models of infective wounds were established after anesthesia. Five circular infective incisions were generated on the back of each rabbit, with a diameter of 2 cm. Five wounds of each rabbit were assigned respectively to the control group, model group, traditional Chinese medicine (TCM) group (Oleum Lithospermum), Western medicine group (calcium alginat), and treatment group (Liuhedan). Wound dressings were performed every day since postoperative day 1. Ten rabbits were selected randomly to be euthanized on postoperative day 3, 7, 14 and 21, respectively. Each specimen was divided into two parts. One was used for detecting interleukin-1β (IL-1β) by enzyme-linked immunosorbent assay, and the other was used for detecting tumor necrosis factor-α (TNF-α) by immunocytochemistry. Results On postoperative day 3 and 7, groups with the expression of IL-1β from low to high were respectively the control group, the treatment group, the Western medicine group, the TCM group, and the model group [postoperative day 3: (680.81±185.53), (1 028.67±205.57), (1 278.67±251.15), (1 449.86±230.74), (1 544.62±371.77) pg/mL; postoperative day 7: (1 024.43±239.94), (1 333.57±257.31), (1 635.14±222.40), (1 784.71±323.85), (1 953.29±324.78) pg/mL], and all the differences among the groups were significant (P<0.05); On postoperative day 14, groups with the expression of IL-1β from low to high were respectively the treatment group, the control group, the Western medicine group, the TCM group, and the model group [(908.71±108.61), (978.57±161.75), (1 120.43±265.39), (1 129.71±298.06), (1 191.14±234.92) pg/mL], and all the differences among groups were significant (P<0.05) except the difference between the Western medicine group and the TCM group (P>0.05); On postoperative day 21, the expression of IL-1β in the control group, the model group, the TCM group, and the Western medicine group was (487.19±121.80), (496.35±102.15), (500.31±139.34), (499.08±120.67) pg/mL, respectively, with no significant differences among the groups (P>0.05), which were all higher than that in the treatment group [(398.62±102.93) pg/mL] with significant difference (P<0.05). The expression of TNF-α in the model group was significantly greater than those in the other groups. The expression of TNF-α in the treatment group and Western medicine group was significantly lower compared with the model group. The expression of TNF-α in the TCM group was stronger compared with those in the treatment group and the Western medicine group. Conclusion Liuhedan can specifically suppress the expressions of IL-1β and TNF-α in the treatment of infective wounds, decrease the release of inflammatory factor and promote the healing.

Citation： LÜ Changyao, WU Jing, HE Hongbo. The impact of Liuhedan on interleukin-1β and tumor necrosis factor-alpha during infective wound healing in rabbits. West China Medical Journal, 2017, 32(10): 1544-1550. doi: 10.7507/1002-0179.201703100 Copy

1.	熊艳艳, 张刘娜, 陈本会. 外用六合丹湿敷治疗急性嵌顿痔水肿的疗效观察. 华西医学, 2016, 31(5): 955-957.
2.	李辉香, 陈本会. 中药六合丹外敷对混合痔外剥内扎术后水肿的效果评价. 华西医学, 2015, 30(7): 1280-1281.
3.	李黎, 邱生林, 岳晓燕. 六合丹外敷治疗流行性腮腺炎患儿腮腺及睾丸肿大的疗效观察及护理. 2016, 38(1): 113-116.
4.	叶静, 黎贵湘. 六合丹外敷治疗早期哺乳期乳腺炎的疗效观察. 现代中西医结合杂志, 2013, 22(2): 185-186.
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6.	Liu D, Huang Y, Chen B, et al. Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma. Cancer, 2011, 117(16): 3763-3773.
7.	李芬, 李巍, 舒勤. TGF-β 与烧伤创面愈合. 创伤外科杂志, 2016, 18(12): 756-761.
8.	Arend WR. The balance between IL-1 and IL-1Ra in disease. Cytokine Growth Factor Rev, 2002, 13(4/5): 323-340.
9.	石磊. 白介素 1 与幽门螺旋杆菌研究进展. 河北医学, 2010, 16(10): 1252-1254.
10.	Kapadi AS, Lee J, Torre-Amione G, et al. Tumor necrosis factor-alpha gene and protein expression in adult feline myocardium after endotoxin administration. J Clin Invest, 1995, 96(2): 1042-1052.
11.	秦建中, 秦俭, 王光. TNF-α 对表皮细胞增殖的影响. 临床皮肤科杂志, 1995(1): 3-4.
12.	Yew TL, Hung YT, Li HY, et al. Enhancement of wound healing by human multipotent stromal cell conditioned medium: the paracrine factors and p38 MAPK activation. Cell Transplant, 2011, 20(5): 693-706.
13.	吴洋, 周树夏, 苗林. 枪弹伤口软组织中 IL-6、IL-8、TNF-α 变化的免疫组化研究. 第二军医大学学报, 2001, 22(12): 1117-1118.
14.	彭小航, 黄伟, 竹林, 等. 微米六合丹外敷对急性胰腺炎大鼠氧化应激及炎症反应水平的影响. 四川大学学报: 医学版, 2013, 44(6): 978-981.
15.	彭小航, 赵先林, 刘怡玲, 等. 不同六合丹外敷对急性胰腺炎大鼠血浆 TXB2、6-keto-PGF1α 的影响. 山东中医药大学学报, 2013, 37(3): 251-256.

1. 熊艳艳, 张刘娜, 陈本会. 外用六合丹湿敷治疗急性嵌顿痔水肿的疗效观察. 华西医学, 2016, 31(5): 955-957.
2. 李辉香, 陈本会. 中药六合丹外敷对混合痔外剥内扎术后水肿的效果评价. 华西医学, 2015, 30(7): 1280-1281.
3. 李黎, 邱生林, 岳晓燕. 六合丹外敷治疗流行性腮腺炎患儿腮腺及睾丸肿大的疗效观察及护理. 2016, 38(1): 113-116.
4. 叶静, 黎贵湘. 六合丹外敷治疗早期哺乳期乳腺炎的疗效观察. 现代中西医结合杂志, 2013, 22(2): 185-186.
5. 中华医学会消化病学分会, 房静远, 刘文忠, 等. 中国慢性胃炎共识意见(2012 年, 上海). 中华消化杂志, 2013, 33(1): 5-16.
6. Liu D, Huang Y, Chen B, et al. Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma. Cancer, 2011, 117(16): 3763-3773.
7. 李芬, 李巍, 舒勤. TGF-β 与烧伤创面愈合. 创伤外科杂志, 2016, 18(12): 756-761.
8. Arend WR. The balance between IL-1 and IL-1Ra in disease. Cytokine Growth Factor Rev, 2002, 13(4/5): 323-340.
9. 石磊. 白介素 1 与幽门螺旋杆菌研究进展. 河北医学, 2010, 16(10): 1252-1254.
10. Kapadi AS, Lee J, Torre-Amione G, et al. Tumor necrosis factor-alpha gene and protein expression in adult feline myocardium after endotoxin administration. J Clin Invest, 1995, 96(2): 1042-1052.
11. 秦建中, 秦俭, 王光. TNF-α 对表皮细胞增殖的影响. 临床皮肤科杂志, 1995(1): 3-4.
12. Yew TL, Hung YT, Li HY, et al. Enhancement of wound healing by human multipotent stromal cell conditioned medium: the paracrine factors and p38 MAPK activation. Cell Transplant, 2011, 20(5): 693-706.
13. 吴洋, 周树夏, 苗林. 枪弹伤口软组织中 IL-6、IL-8、TNF-α 变化的免疫组化研究. 第二军医大学学报, 2001, 22(12): 1117-1118.
14. 彭小航, 黄伟, 竹林, 等. 微米六合丹外敷对急性胰腺炎大鼠氧化应激及炎症反应水平的影响. 四川大学学报: 医学版, 2013, 44(6): 978-981.
15. 彭小航, 赵先林, 刘怡玲, 等. 不同六合丹外敷对急性胰腺炎大鼠血浆 TXB2、6-keto-PGF1α 的影响. 山东中医药大学学报, 2013, 37(3): 251-256.

West China Medical Journal

The impact of Liuhedan on interleukin-1β and tumor necrosis factor-alpha during infective wound healing in rabbits

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