Expression of Galectin-3 and Galectin-1 in pancreatic cancer_West China Medical Journal

Authors：

 ZHANG Miao ^1,1 , ZHOU Guoxiong ^2,2

1. Department of Gastroenterology, Binhai People’s Hospital, Binhai, Jiangsu 224500, P. R. China;
2. Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P. R. China;

Corresponding author：

ZHANG Miao, Email: 36977339@qq.com

Keywords：

Galectin-3; Galectin-1; Pancreatic cancer

DOI：

10.7507/1002-0179.201708009

Video：

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Objective To observe the expression of Galectin-3 and Galectin-1 in pancreatic cancer and explore the relationship between the expression and pathological grading. Methods Forty specimens of pancreatic carcinoma tissue and thirty-one specimens of normal pancreas tissue were selected, which were confirmed by surgical resection and pathology from 2002 to 2009. The expression of Galectin-3 mRNA and Galectin-1 mRNA in pancreatic cancer cell lines SW1990, PANC-1 and ASPC -1 was detected by means of reverse transcriptase-polymerase chain reaction; the expression of Galectin-3 protein and Galectin-1 protein in SW1990, PANC-1 and ASPC-1 was detected by means of immunocytochemistry; the expression of Galectin-3 protein and Galectin-1 protein in pancreatic cancer and normal pancreatic tissue was detected by means of immunohistochemistry. Results In SW1990, PANC-1 and ASPC-1, Galectin-3 mRNA signal and protein were detected, but no Galectin-1 mRNA signal or protein was detected. There was no expression of Galectin-3 protein or Galectin-1 protein in the 31 specimens of normal pancrease tissue, while there were Galectin-3 protein and Galectin-1 protein expressed in the 40 specimens of pancreatic cancer tissue. In the 40 specimens of pancreatic cancer tissue, the expression of Galectin-3 protein was observed in pancreatic cancer cells, but not in fibroblasts or matrix cells around the cancer mass; while the expression of Galectin-1 protein was observed in fibroblasts and matrix cells around the cancer mass, but not observed in pancreatic cancer cells. There was no significant association between the expression of Galectin-3 protein in pancreatic cancer and pathological grading (P>0.05); while the expression of Galectin-1 protein in pancreatic cancer was related to the pathological grading, and the expression of Galectin-1 protein was significant higher in poorly differentiated tumors than that in well/moderately differentiated tumors (P<0.05). Conclusions Galectin-3 or Galectin-1 is not expressed in normal pancreases; Galectin-3 is expressed in pancreatic cancer cells; Galectin-1 is expressed in fibroblasts and matrix cells around the cancer mass. The expression of Galectin-1 is related with the differentiation of pancreatic cancer.

Citation： ZHANG Miao, ZHOU Guoxiong. Expression of Galectin-3 and Galectin-1 in pancreatic cancer. West China Medical Journal, 2017, 32(10): 1509-1514. doi: 10.7507/1002-0179.201708009 Copy

1.	Wang L, Yang GH, Lu XH, et al. Pancreatic cancer mortality in China (1991-2000). World J Gastroenterol, 2003, 9(8): 1819-1823.
2.	Woo HJ, Joo HG, Song SW, et al. Immunohistochemical detection of galectin-3 in canine gastric carcinomas. J Comp Pathol, 2001, 124(2/3): 216-218.
3.	张楠, 丁彦青, 梁莉. Galectin-3 在大肠癌组织中的表达及其临床意义. 南方医科大学学报, 2006, 26(12): 1685-1689.
4.	Soslow RA, Dannenberg AJ, Rush D, et al. COX-2 is expressed in human pulmonary, colonic, and mammary tumors. Cancer, 2000, 89(12): 2637-2645.
5.	Gong HC, Honjo Y, Nangia-Makker P, et al. The NH<sub>2</sub> terminus of galectin-3 governs cellular compartmentalization and functions in cancer cells. Cancer Res, 1999, 59(24): 6239-6245.
6.	杨志明, 伍晓汀, 何涛. Galectin-3 与肿瘤转移. 中国普外基础与临床杂志, 2005, 12(3): 310-313.
7.	Kawachi K, Matsushita Y, Yonezawa S, et al. Galectin-3 expression in various thyroid neoplasms and its possible role in metastasis formation. Hum Pathol, 2000, 31(4): 428-433.
8.	Idikio H. Galectin-3 expression in human breast carcinoma: correlation with cancer histologic grade. Int J Oncol, 1998, 12(6): 1287-1290.
9.	Danguy A, Camby I, Kiss R. Galectins and cancer. Biochim Biophys Acta, 2002, 1572(2/3): 285-293.
10.	van den Brûle FA, Waltregny D, Castronovo V. Increased expression of galectin-1 in carcinoma-associated stroma predicts poor outcome in prostate carcinoma patients. J Pathol, 2001, 193(1): 80-87.
11.	Allen HJ, Sucato D, Woynarowska B, et al. Role of galaptin in ovarian carcinoma adhesion to extracellular matrix <italic>in vitro</italic>. J cell Biochem, 1990, 43(1):43-57.
12.	Jung EJ, Moon HG, Park ST, et al. Galectin-1 expression in human breast cancer tissues. Eur J Cancer Suppl, 2006m 4(2): 146.
13.	童华生, 赖晓嵘, 张亚历, 等. 大肠癌细胞外基质 galectin-1 梯度表达的研究. 现代肿瘤医学, 2009, 17(2): 288-291.
14.	Berberat PO, Friess H, Wang L, et al. Comparative analysis of galectins in primary tumors and tumor metastasis in human pancreatic cancer. J Histochem Cytochem, 2001, 49(4): 539-549.
15.	Shen J, Person MD, Zhu J, et al. Protein expression profiles in pancreatic adenocarcinoma compared with normal pancreatic tissue and tissue affected by pancreatitis as detected by two-dimensional gel electrophoresis and mass spectrometry. Cancer Res, 2004, 64(24): 9018-9026.

1. Wang L, Yang GH, Lu XH, et al. Pancreatic cancer mortality in China (1991-2000). World J Gastroenterol, 2003, 9(8): 1819-1823.
2. Woo HJ, Joo HG, Song SW, et al. Immunohistochemical detection of galectin-3 in canine gastric carcinomas. J Comp Pathol, 2001, 124(2/3): 216-218.
3. 张楠, 丁彦青, 梁莉. Galectin-3 在大肠癌组织中的表达及其临床意义. 南方医科大学学报, 2006, 26(12): 1685-1689.
4. Soslow RA, Dannenberg AJ, Rush D, et al. COX-2 is expressed in human pulmonary, colonic, and mammary tumors. Cancer, 2000, 89(12): 2637-2645.
5. Gong HC, Honjo Y, Nangia-Makker P, et al. The NH<sub>2</sub> terminus of galectin-3 governs cellular compartmentalization and functions in cancer cells. Cancer Res, 1999, 59(24): 6239-6245.
6. 杨志明, 伍晓汀, 何涛. Galectin-3 与肿瘤转移. 中国普外基础与临床杂志, 2005, 12(3): 310-313.
7. Kawachi K, Matsushita Y, Yonezawa S, et al. Galectin-3 expression in various thyroid neoplasms and its possible role in metastasis formation. Hum Pathol, 2000, 31(4): 428-433.
8. Idikio H. Galectin-3 expression in human breast carcinoma: correlation with cancer histologic grade. Int J Oncol, 1998, 12(6): 1287-1290.
9. Danguy A, Camby I, Kiss R. Galectins and cancer. Biochim Biophys Acta, 2002, 1572(2/3): 285-293.
10. van den Brûle FA, Waltregny D, Castronovo V. Increased expression of galectin-1 in carcinoma-associated stroma predicts poor outcome in prostate carcinoma patients. J Pathol, 2001, 193(1): 80-87.
11. Allen HJ, Sucato D, Woynarowska B, et al. Role of galaptin in ovarian carcinoma adhesion to extracellular matrix <italic>in vitro</italic>. J cell Biochem, 1990, 43(1):43-57.
12. Jung EJ, Moon HG, Park ST, et al. Galectin-1 expression in human breast cancer tissues. Eur J Cancer Suppl, 2006m 4(2): 146.
13. 童华生, 赖晓嵘, 张亚历, 等. 大肠癌细胞外基质 galectin-1 梯度表达的研究. 现代肿瘤医学, 2009, 17(2): 288-291.
14. Berberat PO, Friess H, Wang L, et al. Comparative analysis of galectins in primary tumors and tumor metastasis in human pancreatic cancer. J Histochem Cytochem, 2001, 49(4): 539-549.
15. Shen J, Person MD, Zhu J, et al. Protein expression profiles in pancreatic adenocarcinoma compared with normal pancreatic tissue and tissue affected by pancreatitis as detected by two-dimensional gel electrophoresis and mass spectrometry. Cancer Res, 2004, 64(24): 9018-9026.

West China Medical Journal

Expression of Galectin-3 and Galectin-1 in pancreatic cancer

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