Analysis of drug resistance of staphylococci in Whire Union Bacterial Resistance Surveillance Network across Sichuan from 2015 to 2018_West China Medical Journal

Authors：

WU Xianli ^1,2 , LI Jing ¹ , AO Maocheng ¹ , PANG Zaiyuan ² ,  XIE Yi ¹

1. Department of Experimental Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Department of Clinical Laboratory, Center Hospital of Panzhihua, Panzhihua, Sichuan 617000, P. R. China;

Corresponding author：

XIE Yi, Email: xie_yi_77@163.com

Keywords：

Staphylococci; Staphylococcus aureus; Coagulase-negative staphylococci; Methicillin-resistant coagulase-negative staphylococci; Methicillin-resistant Staphylococcus aureus

DOI：

10.7507/1002-0179.201907074

Video：

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Objective To analyze the characteristics of distribution and drug resistance of clinical isolated staphylococci in the Whire Union Bacterial Resistance Surveillance Network across Sichuan from 2015 to 2018, so as to provide reference for clinical rational drug use and management of drug-resistant bacteria in Sichuan.Methods A total of 18 023 strains of staphylococci were isolated from 9 hospitals of Whire Union Bacterial Resistance Surveillance Network for four years (2015-2018). Drug susceptibility test was carried out by disk diffusion method or automated instrument method. The data were statistically analyzed by WHONET 5.6 according to CLSI 2016 standard.Results The 18 023 strains of staphylococci included 10 865 (60.28%) Staphylococcus aureus and 7 158 (39.72%) coagulase negative staphylococci. No strains resistant to vancomycin and linezolid were found. The detection rates of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci were 25.10% (2 727/ 10 865) and 75.60% (5 411/7 158), respectively. The sensitivity of methicillin-resistant staphylococci to most antibiotics was significantly lower than that of methicillin-sensitive strains (P<0.05). The susceptibility rate of staphylococci to some antibiotics was significantly different from 2015 to 2018(P<0.05). The susceptibility rates of Staphylococcus aureus from different samples to rifampicin, moxifloxacin, ciprofloxacin, levofloxacin, oxacillin and erythromycin were significantly different (P<0.05). The susceptibility rates of Staphylococcus aureus from different departments in different samples of sulfamethoxazole, rifampicin, moxifloxacin, ciprofloxacin, levofloxacin, oxacillin, gentamicin, tetracycline, clindamycin and erythromycin were significantly different (P<0.05).Conclusions The susceptibility of strains isolated from different periods, different specimens and departments to the same antimicrobial agents varies greatly. For the infection of staphylococci, we should use drugs under the guidance of drug susceptibility according to the source of samples, which can avoid the abuse of beta-lactam drugs. Strengthening the monitoring and control of drug-resistant bacteria can prevent or reduce the spread of drug-resistant bacteria.

Citation： WU Xianli, LI Jing, AO Maocheng, PANG Zaiyuan, XIE Yi. Analysis of drug resistance of staphylococci in Whire Union Bacterial Resistance Surveillance Network across Sichuan from 2015 to 2018. West China Medical Journal, 2019, 34(8): 900-906. doi: 10.7507/1002-0179.201907074 Copy

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4.	郭瑞林, 李凯旋, 苏冰, 等. 新生儿呼吸道分离凝固酶阴性葡萄球菌耐药性分析. 检验医学与临床, 2015, 12(3): 322-323, 326.
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1. 倪语星, 尚红. 临床微生物学检验. 5版. 北京: 人民卫生出版社, 2012: 80-81.
2. Clinical and Laboratory Standards Institute. M100S: performance standards for antimicrobial susceptibility testing. 26th Edition, 2016.(2016-01)[2019-07-05]. http://ljzx.cqrmhospital.com/upfiles/201601/20160112155335884.pdf.
3. 胡洪华, 杨永长, 肖代雯, 等. 凝固酶阴性葡萄球菌生物被膜形成及耐药性分析. 中国感染控制杂志, 2015, 14(11): 721-725.
4. 郭瑞林, 李凯旋, 苏冰, 等. 新生儿呼吸道分离凝固酶阴性葡萄球菌耐药性分析. 检验医学与临床, 2015, 12(3): 322-323, 326.
5. 李艳, 杨崇勤. 改进皮肤与瓶盖消毒方法对血培养凝固酶阴性葡萄球菌检出率的影响. 中国感染控制杂志, 2016, 15(5): 324-326.
6. 李明友, 林茂锐, 黄基伟, 等. 血培养和血清降钙素原检测对血流感染者早期诊治的临床价值. 国际检验医学杂志, 2015, 36(10): 1381-1382.
7. 黄有平, 李珺. 血培养检出病原菌分布及双份血培养法的价值分析. 国际检验医学杂志, 2015, 36(20): 2994-2996.
8. 陈海滨, 单文治, 周光, 等. 骨科病房凝固酶阴性葡萄球菌耐药性分析. 中国药业, 2009, 18(9): 59-60.
9. 赵珉, 戴宏, 沈继录, 等. 132例腹膜透析相关性腹膜炎病原菌及其耐药性分析. 中国感染与化疗杂志, 2015, 15(3): 236-243.
10. 方小敏, 李小冰, 郭兆旺. 2014-2015年我院凝固酶阴性葡萄球菌的分布及耐药性分析. 临床合理用药杂志, 2016, 9(10B): 7-8.
11. 阳苹, 夏云. 葡萄球菌感染的临床分布及耐药性分析. 重庆医学, 2012, 41(30): 3167-3168, 3170.
12. 朱德妹, 胡付品, 汪复, 等. 2009年中国CHINET葡萄球菌属细菌耐药性监测. 中国感染与化疗杂志, 2010, 10(6): 414-420.
13. 刘青, 樊冰. 儿童呼吸道感染患者金黄色葡萄球菌的分离与耐药性分析. 现代检验医学杂志, 2016, 31(3): 150-152, 155.
14. 胡付品, 郭燕, 朱德妹, 等. 2016年中国CHINET细菌耐药性监测. 中国感染与化疗杂志, 2017, 17(5): 481-491.
15. 石秀梅, 朱光发, 王爱萍. 509株金黄色葡萄球菌感染的临床分布和耐药性分析. 临床肺科杂志, 2016, 21(8): 1381-1384.
16. Sun W, Chen H, Liu Y, et al. Prevalence and characterization of heterogeneous vancomycin-intermediate Staphylococcus aureus isolates from 14 gitics in China. Antimicrob Agents Chemother, 2009, 53(9): 3642-3649.
17. 孙闻嘉. 我国异质性万古霉素中介的金黄色葡萄球菌（hVISA）的发生率、分子流行病学及病例对照研究. 北京: 中国协和医科大学, 2008.
18. 王敬华, 周惠琴, 杨欢, 等. 利奈唑胺对h-VRS体外抗菌活性研究. 中国抗生素杂志, 2009, 34(9): 570-574.
19. 孙敏, 徐永成, 苗祖豪. 金黄色葡萄球菌的分布特征及耐药性分析. 国际检验医学杂志, 2016, 37(16): 2310-2313.
20. 姚明, 关立锋, 吴银生, 等. 甲氧西林耐药金黄色葡萄球菌基因分型及耐药现状研究. 中华烧伤杂志, 2014, 30(5): 428-432.

West China Medical Journal

Analysis of drug resistance of staphylococci in Whire Union Bacterial Resistance Surveillance Network across Sichuan from 2015 to 2018

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