Effects of different exercise patterns on serum short-chain fatty acids in type 2 diabetic mice_West China Medical Journal

Authors：

MA Cui ¹ , HUANG Yanfeng ¹ , ZHAI Lu ² , LIU Yuhua ³ , ZHANG Qiu ¹ , HUANG Shan ¹ , ZHONG Xin ¹ ,  DAI Xia ¹

1. Department of Nursing, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P. R. China;
2. Stem Cell Transplantation Division, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P. R. China;
3. Pulmonary and Critical Care Medicine Ward Ⅱ, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P. R. China;

Corresponding author：

DAI Xia, Email: 2655947220@qq.com

Keywords：

Diabetes; Aerobic exercise; Resistance exercise; Short-chain fatty acids

DOI：

10.7507/1002-0179.201910088

Video：

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Objective To explore the effects of different exercise methods on serum short-chain fatty acids (SCFA) in type 2 diabetic mice, determine the best exercise method to improve SCFAs in type 2 diabetic mice, and provide a theoretical basis for the preventive intervention for patients with early diabetes.Methods According to different exercise methods, 48 8-week-old male db/db type 2 diabetic mice were randomly divided into four groups, including aerobic exercise group, resistance exercise group, combined resistance- aerobic exercise (referred to as combined exercise) group, and the control group; with 10 mice in each group and another 2 as the substitutes. The mice were fed in the same manner in each group. The control group did not perform exercise intervention, the aerobic exercise group performed weightless running exercise, the resistance exercise group performed tail weight-bearing ladder exercise, and the combined exercise group alternated aerobic exercise and resistance exercise. Blood glucose and body weight were measured before and 8 weeks after the intervention. The content of serum SCFAs in mice was determined by gas chromatography-mass spectrometry.Results A total of 40 mice completed the experiment successfully. Before the exercise intervention, there was no significant difference in blood glucose or weight among the groups (P>0.05). After 8 weeks of exercise intervention, the blood glucose and weight in each exercise group were significantly lower than those in the control group (P<0.05), and the blood glucose and weight in the combined exercise group were significantly lower than those in the aerobic exercise group and the resistance exercise group (P<0.05). The contents of SCFA were higher in the aerobic exercise group, resistance exercise group, and combined exercise group than those in the control group (P<0.05); the contents of acetic acid and butyric acid in the combined exercise group were better than those in the aerobic exercise group (P<0.05), and the contents of propanoic acid and valeric acid in the combined exercise group were better than those in the resistance exercise group (P<0.05).Conclusions Different exercise methods can improve the SCFA content in serum of type 2 diabetic mice. Compared with aerobic exercise and resistance exercise, combined exercise has the best effect in improving SCFA.

Citation： MA Cui, HUANG Yanfeng, ZHAI Lu, LIU Yuhua, ZHANG Qiu, HUANG Shan, ZHONG Xin, DAI Xia. Effects of different exercise patterns on serum short-chain fatty acids in type 2 diabetic mice. West China Medical Journal, 2020, 35(11): 1370-1375. doi: 10.7507/1002-0179.201910088 Copy

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19.	Allen JM, Mailing LJ, Niemiro GM, et al. Exercise alters gut microbiota composition and function in lean and obese humans. Med Sci Sports Exerc, 2018, 50(4): 747-757.
20.	陆丽荣, 戴霞, 韦薇, 等. 联合抗阻-有氧运动对糖尿病前期人群糖及脂代谢指标的影响. 护理研究(上旬版), 2016, 30(4): 1230-1233.
21.	陆丽荣. 联合抗阻-有氧运动对糖尿病前期人群代谢控制影响的研究. 南宁: 广西医科大学, 2016.
22.	Kutikhin AG, Sinitsky MY, Yuzhalin AE, et al. Shear stress: an essential driver of endothelial progenitor cells. J Mol Cell Cardiol, 2018, 118: 46-69.
23.	Su SH, Wu CH, Chiu YL, et al. Dysregulation of vascular endothelial growth factor receptor-2 by multiple miRNAs in endothelial colony-forming cells of coronary artery disease. J Vas Res, 2017, 54(1): 22-32.
24.	刘玉花. Caveolin-1 对 2 型糖尿病小鼠内皮祖细胞功能的影响研究. 南宁: 广西医科大学, 2019.
25.	刘玉花, 翟露, 黄燕凤, 等. 运动对 2 型糖尿病小鼠内皮祖细胞功能的影响. 现代预防医学, 2019, 46(13): 2424-2430, 2444.

1. Noble D, Mathur R, Dent T, et al. Risk models and scores for type 2 diabetes: systematic review. BMJ, 2011, 343: d7163.
2. Qin J, Li Y, Cai Z, et al. A metagenome-wide association study of gut microbiota in type 2 diabetes. Nature, 2012, 490(7418): 55-60.
3. Upadhyaya S, Banerjee G. Type 2 diabetes and gut microbiome: at the intersection of known and unknown. Gut microbes, 2015, 6(2): 85-92.
4. American Diabetes Association. Standards of medical care in diabetes--2012. Diabetes care, 2012, 35(Suppl 1): S11-S63.
5. Morrison F, Shubina M, Turchin A. Lifestyle counseling in routine care and long-term glucose, blood pressure, and cholesterol control in patients with diabetes. Diabetes care, 2012, 35(2): 334-341.
6. Li Y, Xu S, Zhang X, et al. Skeletal intramyocellular lipid metabolism and insulin resistance. Biophys Reports, 2015, 1: 90-98.
7. 杨晓庆, 李琳琳, 王烨. 小鼠肠道菌群代谢产物与糖尿病的相关性研究. 中国微生态学杂志, 2011, 23(2): 134-136, 140.
8. 胡娟, 吴洪斌, 郑刚, 等. 可溶性膳食纤维对高脂膳食大鼠血清短链脂肪酸的影响. 食品科学, 2011, 32(17): 321-325.
9. Zar Kalai F, Han J, Ksouri R, et al. Oral administration of Nitraria retusa ethanolic extract enhances hepatic lipid metabolism in db/db mice model ‘BKS. Cg-Dock7(m)+/+ Lepr(db/)J’ through the modulation of lipogenesis-lipolysis balance. Food Chem Toxicol, 2014, 72: 247-256.
10. Speakman J, Hambly C, Mitchell S, et al. Animal models of obesity. Obes Rev, 2007, 8(Suppl 1): 55-61.
11. 陆丽荣, 戴霞, 陈青云, 等. 联合抗阻—有氧运动对糖尿病前期人群代谢指标的影响. 广西医科大学学报, 2016, 33(1): 57-59.
12. 赵文飘. 联合运动对增强2型糖尿病小鼠内皮袓细胞功能相关Caveolin-1/PI3K/Akt蛋白表达的影响研究. 南宁: 广西医科大学, 2018.
13. 孟晴, 陈伟, 张明, 等. 有氧联合抗阻运动对 2 型糖尿病患者的效果. 中国康复理论与实践, 2018, 24(12): 1465-1470.
14. Ratajczak W, Rył A, Mizerski A, et al. Immunomodulatory potential of gut microbiome-derived short-chain fatty acids (SCFAs). Acta Biochim Pol, 2019, 66(1): 1-12.
15. Canfora EE, Jocken JW, Blaak EE. Short-chain fatty acids in control of body weight and insulin sensitivity. Nat Rev Endocrinol, 2015, 11(10): 577-591.
16. Al-Lahham SH, Roelofsen H, Priebe M, et al. Regulation of adipokine production in human adipose tissue by propionic acid. Eur J Clin Invest, 2010, 40(5): 401-407.
17. Rakitin A, Eglit T, Kõks S, et al. Comparison of the metabolic syndrome risk in valproate-treated patients with epilepsy and the general population in Estonia. PLoS One, 2014, 9(7): e103856.
18. 潘虹, 王俏梅. 高脂膳食所致大鼠高血糖及其与肠道菌群, 代谢产物的相关性实验研究. 药物分析杂志, 2019, 39(2): 280-285.
19. Allen JM, Mailing LJ, Niemiro GM, et al. Exercise alters gut microbiota composition and function in lean and obese humans. Med Sci Sports Exerc, 2018, 50(4): 747-757.
20. 陆丽荣, 戴霞, 韦薇, 等. 联合抗阻-有氧运动对糖尿病前期人群糖及脂代谢指标的影响. 护理研究(上旬版), 2016, 30(4): 1230-1233.
21. 陆丽荣. 联合抗阻-有氧运动对糖尿病前期人群代谢控制影响的研究. 南宁: 广西医科大学, 2016.
22. Kutikhin AG, Sinitsky MY, Yuzhalin AE, et al. Shear stress: an essential driver of endothelial progenitor cells. J Mol Cell Cardiol, 2018, 118: 46-69.
23. Su SH, Wu CH, Chiu YL, et al. Dysregulation of vascular endothelial growth factor receptor-2 by multiple miRNAs in endothelial colony-forming cells of coronary artery disease. J Vas Res, 2017, 54(1): 22-32.
24. 刘玉花. Caveolin-1 对 2 型糖尿病小鼠内皮祖细胞功能的影响研究. 南宁: 广西医科大学, 2019.
25. 刘玉花, 翟露, 黄燕凤, 等. 运动对 2 型糖尿病小鼠内皮祖细胞功能的影响. 现代预防医学, 2019, 46(13): 2424-2430, 2444.

West China Medical Journal

Effects of different exercise patterns on serum short-chain fatty acids in type 2 diabetic mice

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